Cafestol palmitate
Cafestol palmitate is an orally active diterpene ester. Cafestol palmitate can be isolated from raw coffee beans. Cafestol palmitate enhances the activity of Glutathione S-transferase. Cafestol palmitate downregulates the expression of Akt and VEGFR2. Cafestol palmitate can be used in the research of inflammatory diseases and cardiovascular diseases.
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- CAS No.: 81760-46-5
- Formule: C36H58O4
- Masse moléculaire:554.84
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
IC50 & Target
[2]|
VEGFR2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| RAW264.7 | IC50 |
>10 μg/mL
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Inhibition of cyclooxygenase-2 (COX-2) activity in LPS-induced RAW 264.7 mouse macrophages assessed via measuring PGE2 levels in supernatants by enzyme immunoassay, with 2 h incubation of test samples post-COX-2 induction.
Inhibition of cyclooxygenase-2 (COX-2) activity in LPS-induced RAW 264.7 mouse macrophages assessed via measuring PGE2 levels in supernatants by enzyme immunoassay, with 2 h incubation of test samples post-COX-2 induction.
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1934578X0800300103 |
| RAW264.7 | IC50 |
>50 μg/mL
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Antiproliferative activity against RAW 264.7 mouse macrophages assessed as reduction in cell viability incubated for 48 hrs by XTT assay.
Antiproliferative activity against RAW 264.7 mouse macrophages assessed as reduction in cell viability incubated for 48 hrs by XTT assay.
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1934578X0800300103 |
| HL-60 | IC50 |
>50 μg/mL
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Antiproliferative activity against HL-60 myelomonocytic cells assessed as reduction in cell viability by MTT assay.
Antiproliferative activity against HL-60 myelomonocytic cells assessed as reduction in cell viability by MTT assay.
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1934578X0800300103 |
| HL-60 | IC50 |
>62.5 μg/mL
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Antiproliferative activity against HL-60 myelomonocytic cells assessed as reduction in cell viability with 10 min pre-incubation before PMA addition, 16 h total incubation with PMA, by XTT assay.
Antiproliferative activity against HL-60 myelomonocytic cells assessed as reduction in cell viability with 10 min pre-incubation before PMA addition, 16 h total incubation with PMA, by XTT assay.
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1934578X0800300103 |
In Vitro
Cafestol palmitate (50 μM) inhibits the proliferation and migration of human microvascular endothelial cells by down-regulating VEGFR2 and Akt expression[2].
Cafestol palmitate (2 h) weakly inhibits COX-2 activity in LPS-induced RAW 264.7 mouse macrophages with an IC50 >10 μg/mL and shows no cytotoxicity in this cell line[3].
Cafestol palmitate (48 h) does not exhibit significant cytotoxicity in RAW 264.7 mouse macrophages, with a cell proliferation IC50 >50 μg/mL[3].
Cafestol palmitate does not exhibit significant cytotoxicity in HL-60 myelomonocytic cells, with a cell proliferation IC50 >50 μg/mL[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:RAW 264.7 mouse macrophages
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Concentration:Unclear
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Incubation Time:48 h
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Result:Showed no significant cytotoxicity, with a cell proliferation IC50 value greater than 50 μg/mL.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ICR/Ha (female, 7 weeks of age)[1]
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Dosage:2.5 mg/mouse (single dose, marginal activity); 2.5 mg/mouse (4 daily doses, 41% increase); 5.0 mg/mouse (4 daily doses, 3.9x control)
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Administration:p.o.; single dose (killed 28h later); once daily for 4 days (killed 24h after last dose)
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Result:Resulted in marginal GSH S-transferase activity in small bowel mucosa.
Increased small bowel mucosa GSH S-transferase activity by 41% relative to controls.
Increased small bowel mucosa GSH S-transferase activity to 3.9 times the control level.
Chemical Information
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CAS No. 81760-46-5
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Masse moléculaire 554.84
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Formule C36H58O4
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SMILES
C[C@]12[C@@]3([H])[C@@]4(CC[C@]1([H])C5=C(OC=C5)CC2)C[C@](CC3)([H])[C@](O)(C4)COC(CCCCCCCCCCCCCCC)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
Pureté et documentation
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Fiche technique (276 KB)
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SDS (252 KB)
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- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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