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Cat. No.: HY-135124

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DC44SMe

DC44SMe, a phosphate proagent of cytotoxic DNA alkylator DC44, can be used in the synthesis of antibody-drug conjugate (ADC). DC44SMe exhibits IC₅₀s of 2.0 nM, 2.8 nM, and 1.9 nM for Ramos, Namalwa, and HL60/s cancer cells, respectively. DC44SMe can be used for the targeted treatment of cancer.

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CAS No.: 1354787-76-0
Formule: C₃₈H₃₇ClN₅O₇PS₂
Masse moléculaire:806.29

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (267 KB)

DC44SMe Chemical Structure

CAS No.: 1354787-76-0

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Activité biologique
- Description
- Cellular Effect
- In Vitro
Chemical Information
Protocole (1)
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
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Activité biologique

Description

Voir tous les produits spécifiques à Isoform ADC Payloads :

Cellular Effect

Cell Line	Type	Value	Description	References
COLO 205	IC₅₀	>3000 pM Compound: 39, DC44SMe	Cytotoxicity against human COLO205 cells assessed as cell survival after 72 hrs by clonogenic assay Cytotoxicity against human COLO205 cells assessed as cell survival after 72 hrs by clonogenic assay	[PMID: 22148292]
HL-60	IC₅₀	1900 pM Compound: 39, DC44SMe	Cytotoxicity against human HL60 cells assessed as cell survival after 72 hrs by clonogenic assay Cytotoxicity against human HL60 cells assessed as cell survival after 72 hrs by clonogenic assay	[PMID: 22148292]
NAMALVA	IC₅₀	25 pM Compound: 39, DC44SMe	Cytotoxicity against human NAMALWA cells assessed as cell survival after 72 hrs in presence of bovine alkaline phosphatase by clonogenic assay Cytotoxicity against human NAMALWA cells assessed as cell survival after 72 hrs in presence of bovine alkaline phosphatase by clonogenic assay	[PMID: 22148292]
NAMALVA	IC₅₀	2800 pM Compound: 39, DC44SMe	Cytotoxicity against human NAMALWA cells assessed as cell survival after 72 hrs by clonogenic assay Cytotoxicity against human NAMALWA cells assessed as cell survival after 72 hrs by clonogenic assay	[PMID: 22148292]
Ramos	IC₅₀	2000 pM Compound: 39, DC44SMe	Cytotoxicity against human Ramos cells assessed as cell survival after 72 hrs by clonogenic assay Cytotoxicity against human Ramos cells assessed as cell survival after 72 hrs by clonogenic assay	[PMID: 22148292]
Ramos	IC₅₀	90 pM Compound: 39, DC44SMe	Cytotoxicity against human Ramos cells assessed as cell survival after 72 hrs in presence of bovine alkaline phosphatase by clonogenic assay Cytotoxicity against human Ramos cells assessed as cell survival after 72 hrs in presence of bovine alkaline phosphatase by clonogenic assay	[PMID: 22148292]

In Vitro

The phosphate prodrug may be a suitable choice for ADCs of effector molecules that do not possess sufficient water solubility or stability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 1354787-76-0
Masse moléculaire 806.29
Formule C₃₈H₃₇ClN₅O₇PS₂
SMILES

O=C(C(N1)=CC2=C1C=CC(NC(CCC(SSC)(C)C)=O)=C2)NC3=CC4=C(NC(C(N5C[C@@H](CCl)C6=C5C=C(OP(O)(O)=O)C7=CC=CC=C67)=O)=C4)C=C3
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocole

All Cell Biology

Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (267 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Zhao RY, et al. Synthesis and biological evaluation of antibody conjugates of phosphate prodrugs of cytotoxic DNA alkylators for the targeted treatment of cancer. J Med Chem. 2012 Jan 26;55(2):766-82. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Keywords

MOQ

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100 mg

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