KR-32568
Based on 1 Customer Validation
KR-32568 is a sodium/hydrogen exchanger-1 (NHE-1) inhibitor with an IC50 of 230 nM. KR-32568 has cardioprotective effects.
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- Pureté : 99.74%
- CAS No.: 852146-73-7
- Formule: C13H12FN3O2
- Masse moléculaire:261.25
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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HY-19693
CariporideNa+/H+ Exchange Inhibitor -
HY-15991
TenapanorNHE3 Inhibitor -
HY-16022
AcetyldigitoxinCardiac Glycoside -
HY-15991A
Tenapanor hydrochlorideNHE3 Inhibitor -
HY-105064D
Zoniporide hydrochloride hydrateNHE1 Inhibitor -
HY-139313
NHE3-IN-2NHE3 Inhibitor -
HY-106150B
Eniporide hydrochlorideNa+/H+ Exchange Inhibitor -
HY-18071A
BI-9627 hydrochlorideNHE1 Inhibitor -
HY-100302
FR183998 free baseNa+/H+-Exchange Inhibitor -
HY-100325
NHE3-IN-1NHE-3 Inhibitor -
HY-19693R
Cariporide (Standard)Na+/H+ Exchange Inhibitor -
HY-100302R
FR183998 free base (Standard)Na+/H+-Exchange Inhibitor
Activité biologique
Description
IC50 & Target
IC50: 230 nM (NHE-1)[2]
In Vitro
KR-32568 (10 nM-1 μM) inhibits NHE-1-mediated platelet swelling induced by intracellular acidifi cation of rabbit platelet-rich plasma in a concentration-dependent manner, with the IC50 value of 24 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley rats (350-380 g) bearing 30 min ischemia/2.5 h reperfusion heart injury[1]
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Dosage:0.1 mg/kg and 1.0 mg/kg
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Administration:i.v.; once
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Result:Exerted potent cardioprotective effects in rats.
Chemical Information
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CAS No. 852146-73-7
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Appearance Solid
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Masse moléculaire 261.25
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Formule C13H12FN3O2
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Color White to off-white
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SMILES
FC1=CC=C(C(C2=CC=C(O2)C(NC(N)=N)=O)=C1)C
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (382.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Hui-Yul Roh, et al. Cardioprotective effects of [5-(2-methyl-5-fluorophenyl)furan-2-ylcarbonyl]guanidine (KR-32568) in an anesthetized rat model of ischemia and reperfusion heart injury. Pharmacology. 2005 Dec;75(1):37-44. [Content Brief]
[2]. Sunkyung Lee, et al. (5-Arylfuran-2-ylcarbonyl)guanidines as cardioprotectives through the inhibition of Na+/H+ exchanger isoform-1. J Med Chem. 2005 Apr 21;48(8):2882-91. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.8278 mL | 19.1388 mL | 38.2775 mL | 95.6938 mL |
| 5 mM | 0.7656 mL | 3.8278 mL | 7.6555 mL | 19.1388 mL | |
| 10 mM | 0.3828 mL | 1.9139 mL | 3.8278 mL | 9.5694 mL | |
| 15 mM | 0.2552 mL | 1.2759 mL | 2.5518 mL | 6.3796 mL | |
| 20 mM | 0.1914 mL | 0.9569 mL | 1.9139 mL | 4.7847 mL | |
| 25 mM | 0.1531 mL | 0.7656 mL | 1.5311 mL | 3.8278 mL | |
| 30 mM | 0.1276 mL | 0.6380 mL | 1.2759 mL | 3.1898 mL | |
| 40 mM | 0.0957 mL | 0.4785 mL | 0.9569 mL | 2.3923 mL | |
| 50 mM | 0.0766 mL | 0.3828 mL | 0.7656 mL | 1.9139 mL | |
| 60 mM | 0.0638 mL | 0.3190 mL | 0.6380 mL | 1.5949 mL | |
| 80 mM | 0.0478 mL | 0.2392 mL | 0.4785 mL | 1.1962 mL | |
| 100 mM | 0.0383 mL | 0.1914 mL | 0.3828 mL | 0.9569 mL |
KR-32568 Related Classifications
HY-118778 Related Classifications
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