Calmidazolium chloride
Based on 5 publication(s) in Google Scholar
Calmidazolium chloride (R 24571) is a calmodulin antagonist, antagonizing CaM-dependent phosphodiesterase and calmodulin-induced activation of erythrocyte Ca2+-transporting ATPase with IC50s of 0.15 and 0.35 μM, respectively. Also in anti-cancer research. Calmidazolium binds to calmodulin with a Kd of 3 nM.
For research use only. We do not sell to patients.
- Purity: 99.94%
- CAS No.: 57265-65-3
- Formula: C31H23Cl7N2O
- Molecular Weight:687.70
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Calmidazolium chloride
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Customer Validation & Images
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Cell Migration/Invasion Assay
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WB
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Cell Migration/Invasion Assay
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WB
Biological Activity
Kd: 3 nM (Calmodulin)[3]
Calmidazolium chloride is widely used as a calmodulin (CaM) antagonist, but is also known to induce apoptosis in certain cancer cell lines. Calmidazolium chloride (3, 5, 7, 10 μM, 30 minutes-24 hours) inhibits growth of mouse F9 ECCs[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Mouse F9 ECCs
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Concentration:3, 5, 7, 10 μM
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Incubation Time:30 minutes-24 hours
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Result:The IC50s of Calmidazolium chloride treated F9 ECCs and E14 ESCs are 8.18 μM, and 12.69 μM[2].
Chemical Information
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CAS No. 57265-65-3
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Appearance Solid
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Molecular Weight 687.70
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Formula C31H23Cl7N2O
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Color White to off-white
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SMILES
ClC1=CC=C(COC(C2=CC=C(Cl)C=C2Cl)CN3C=C[N+](C(C4=CC=C(Cl)C=C4)C5=CC=C(Cl)C=C5)=C3)C(Cl)=C1.[Cl-]
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Synonyms
R 24571
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Mol Cell
The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells. [Abstract]2025 Oct 29:S1097-2765(25)00822-6. PMID: 41167191 -
Int J Biol Macromol
Genome-wide identification and expression analysis of neuropeptide G protein-coupled receptors in Urechis unicinctus (Annelida, Echiura). [Abstract]2025 Apr 26:143548. PMID: 40294673 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Cell Mol Life Sci
Hypoxia drives cervical cancer progression via OCT4/ORAI3-dependent glycolysis and Ca2+ signaling. [Abstract]2025 Nov 6;82(1):390. PMID: 41196424
Calmidazolium chloride purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Nov 6;82(1):390. [Abstract]
More importantly, the application of Calmidazolium chloride (R24571), the inhibitor of the calcium signaling pathway, could also reverse the OCT4-promoted cell motility of cervical cancer cells.
Calmidazolium chloride purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Nov 6;82(1):390. [Abstract]
OCT4-promoted ORAI3 expression and EMT process, as well as the OCT4-activated signaling pathway, were all recovered by Calmidazolium chloride (R24571) at the protein level.
Calmidazolium chloride purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Nov 6;82(1):390. [Abstract]
More importantly, the application of Calmidazolium chloride (R24571), the inhibitor of the calcium signaling pathway, could also reverse the OCT4-promoted cell motility of cervical cancer cells.
Calmidazolium chloride purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2025 Nov 6;82(1):390. [Abstract]
OCT4-promoted ORAI3 expression and EMT process, as well as the OCT4-activated signaling pathway, were all recovered by Calmidazolium chloride (R24571) at the protein level.
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bioRxiv
Structural Characterization of Calcium-Dependent Calmodulin-Calmidazolium Binding using Capillary Vibrating Sharp-Edge Spray-based Native Mass Spectrometry and In-Droplet Hydrogen Deuterium Exchange Mass Spectrometry. [Abstract]2026 Jun 17:2026.05.15.725515. PMID: 42239371
Solvent & Solubility
DMSO : 100 mg/mL (145.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Gietzen K, et al. Comparison of the calmodulin antagonists compound 48/80 and calmidazolium. Biochem J. 1983 Dec 15;216(3):611-6. [Content Brief]
[2]. Lee J, et al. Calmidazolium chloride inhibits growth of murine embryonal carcinoma cells, a model of cancer stem-like cells. Toxicol In Vitro. 2016 Sep;35:86-92. [Content Brief]
[3]. Budu A, et al. Calmidazolium evokes high calcium fluctuations in Plasmodium falciparum. Cell Signal. 2016 Mar;28(3):125-135. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4541 mL | 7.2706 mL | 14.5412 mL | 36.3531 mL |
| 5 mM | 0.2908 mL | 1.4541 mL | 2.9082 mL | 7.2706 mL | |
| 10 mM | 0.1454 mL | 0.7271 mL | 1.4541 mL | 3.6353 mL | |
| 15 mM | 0.0969 mL | 0.4847 mL | 0.9694 mL | 2.4235 mL | |
| 20 mM | 0.0727 mL | 0.3635 mL | 0.7271 mL | 1.8177 mL | |
| 25 mM | 0.0582 mL | 0.2908 mL | 0.5816 mL | 1.4541 mL | |
| 30 mM | 0.0485 mL | 0.2424 mL | 0.4847 mL | 1.2118 mL | |
| 40 mM | 0.0364 mL | 0.1818 mL | 0.3635 mL | 0.9088 mL | |
| 50 mM | 0.0291 mL | 0.1454 mL | 0.2908 mL | 0.7271 mL | |
| 60 mM | 0.0242 mL | 0.1212 mL | 0.2424 mL | 0.6059 mL | |
| 80 mM | 0.0182 mL | 0.0909 mL | 0.1818 mL | 0.4544 mL | |
| 100 mM | 0.0145 mL | 0.0727 mL | 0.1454 mL | 0.3635 mL |
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