CDC25B-IN-1

Name: CDC25B-IN-1
Brand: MedChemExpress
SKU: HY-126246
Rating: 4.5 (1 reviews)

Cat. No.: HY-126246 Purity: 98.09%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

CDC25B-IN-1 (compound 4a) is a potent inhibitor of cell division cycle 25B (CDC25B) phosphatase, with a K_i of 8.5 μM. CDC25B-IN-1 potently inhibits cell proliferation and colony formation, causes an increase of the G2/M phase.

For research use only. We do not sell to patients.

CDC25B-IN-1 Chemical Structure

CAS No. : 2374831-10-2

Size	Price	Stock	Quantity
Oil + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 308	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 308	In-stock	Estimated Time of Arrival: December 31
Oil
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 440	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 750	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1100	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1450	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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References
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Description

IC₅₀ & Target

Ki: 8.5 μM (CDC25B phosphatase)^[1]

Molecular Weight

321.37

Formula

C₂₀H₁₉NO₃

CAS No.

2374831-10-2

Appearance

Oil

Color

Light brown to brown

SMILES

COC1=CC2=CC=CC=C2C(NC3=CC=CC(C(OCC)=O)=C3)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (311.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (155.58 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1117 mL	15.5584 mL	31.1168 mL
5 mM	0.6223 mL	3.1117 mL	6.2234 mL
10 mM	0.3112 mL	1.5558 mL	3.1117 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.09%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (251 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Cerchia C, et al. Discovery of Novel Naphthylphenylketone and Naphthylphenylamine Derivatives as Cell Division Cycle 25B (CDC25B) Phosphatase Inhibitors: Design, Synthesis, Inhibition Mechanism, and in Vitro Efficacy against Melanoma Cell Lines. J Med Che [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.1117 mL	15.5584 mL	31.1168 mL	77.7920 mL
	5 mM	0.6223 mL	3.1117 mL	6.2234 mL	15.5584 mL
	10 mM	0.3112 mL	1.5558 mL	3.1117 mL	7.7792 mL
	15 mM	0.2074 mL	1.0372 mL	2.0745 mL	5.1861 mL
	20 mM	0.1556 mL	0.7779 mL	1.5558 mL	3.8896 mL
	25 mM	0.1245 mL	0.6223 mL	1.2447 mL	3.1117 mL
	30 mM	0.1037 mL	0.5186 mL	1.0372 mL	2.5931 mL
	40 mM	0.0778 mL	0.3890 mL	0.7779 mL	1.9448 mL
	50 mM	0.0622 mL	0.3112 mL	0.6223 mL	1.5558 mL
	60 mM	0.0519 mL	0.2593 mL	0.5186 mL	1.2965 mL
	80 mM	0.0389 mL	0.1945 mL	0.3890 mL	0.9724 mL
	100 mM	0.0311 mL	0.1556 mL	0.3112 mL	0.7779 mL

CDC25B-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CDC25B-IN-12374831-10-2PhosphataseInhibitorinhibitorinhibit

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CDC25B-IN-1

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Complete Stock Solution Preparation Table

CDC25B-IN-1 Related Classifications

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