Art. -Nr.: HY-16786

AI-10-49

Name: AI-10-49
Brand: MedChemExpress
SKU: HY-16786
Price: 85.00 USD
Availability: InStock
Rating: 5 (1 reviews)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

AI-10-49 is an inhibitor of leukemic oncoprotein CBFβ-SMHHC. AI-10-49 inhibits the binding of CBFβ-SMMHCto the RUNX1 Runt domain with IC₅₀ value of 0.26 μM. AI-10-49 can be used for the research of leukemia.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 98.49%
CAS. Nr.: 1256094-72-0
Formel: C₃₀H₂₂F₆N₆O₅
Molecular Weight:660.52

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

AI-10-49 Chemical Structure

CAS. Nr.: 1256094-72-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle RUNX Isoform-spezifische Produkte anzeigen :

RUNX1
RUNX2

Cellular Effect

Cell Line	Type	Value	Description	References
Caco-2	CC₅₀	6.58 μM Compound: AI-10-49	Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay	10.21203/rs.3.rs-23951/v1
Caco-2	IC₅₀	0.19 μM Compound: AI-10-49	Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging	10.21203/rs.3.rs-23951/v1

In Vitro

AI-10-49 inhibits the binding of CBFβ-SMMHCto theRUNX1 Runt domain with IC₅₀ value of 0.26 μM^[1].
AI-10-49 (1 μM; 3, 6, 12 hours) has selectivity for inhibiting CBFβ-SMMHC binding to RUNX1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

ME-1 cells
Concentration:

1 μM
Incubation Time:

3, 6 hours
Result:

Effectively dissociated RUNX1 from CBFβ-SMMHC.

RT-PCR^[1]

Cell Line:

ME-1 and U937 cells
Concentration:

1 μM
Incubation Time:

6, 12 hours
Result:

Increased expression of RUNX3, CSF1R, and CEBPA.

In Vivo

AI-10-49 (200 mg/kg; per day) delays leukemia progression in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mice (Cbfb^+/MYH11:Ras^+/G12D leukemic cells)^[1]
Dosage:

200 mg/kg
Administration:

per day
Result:

Reduced leukemia expansion in vivo and survived significantly long.

Chemical Information

CAS. Nr. 1256094-72-0
Appearance Solid
Molecular Weight 660.52
Formel C₃₀H₂₂F₆N₆O₅
Color Off-white to yellow
SMILES

FC(F)(F)OC1=CC=C2N=C(C3=NC=C(OCCOCCOC4=CN=C(C5=NC6=CC=C(OC(F)(F)F)C=C6N5)C=C4)C=C3)NC2=C1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (1)

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Journal Impact Factor

Cell Rep

CDK12 inhibition reveals melanoma dependence on the RUNX1/CBFβ complex for genomic stability. [Abstract]

2025 Nov 25;44(11):116495. PMID: 41176765

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (151.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5140 mL

7.5698 mL

15.1396 mL

5 mM

0.3028 mL

1.5140 mL

3.0279 mL

10 mM

0.1514 mL

0.7570 mL

1.5140 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Anuradha Illendula, et al. Chemical biology. A small-molecule inhibitor of the aberrant transcription factor CBFβ-SMMHC delays leukemia in mice. Science. 2015 Feb 13;347(6223):779-84. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.5140 mL	7.5698 mL	15.1396 mL	37.8490 mL
	5 mM	0.3028 mL	1.5140 mL	3.0279 mL	7.5698 mL
	10 mM	0.1514 mL	0.7570 mL	1.5140 mL	3.7849 mL
	15 mM	0.1009 mL	0.5047 mL	1.0093 mL	2.5233 mL
	20 mM	0.0757 mL	0.3785 mL	0.7570 mL	1.8924 mL
	25 mM	0.0606 mL	0.3028 mL	0.6056 mL	1.5140 mL
	30 mM	0.0505 mL	0.2523 mL	0.5047 mL	1.2616 mL
	40 mM	0.0378 mL	0.1892 mL	0.3785 mL	0.9462 mL
	50 mM	0.0303 mL	0.1514 mL	0.3028 mL	0.7570 mL
	60 mM	0.0252 mL	0.1262 mL	0.2523 mL	0.6308 mL
	80 mM	0.0189 mL	0.0946 mL	0.1892 mL	0.4731 mL
	100 mM	0.0151 mL	0.0757 mL	0.1514 mL	0.3785 mL

Bioz

Keywords

AI-10-49

1 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) AI-10-49

Alle RUNX Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

AI-10-49

Ro5-3335

MSA-2

Inobrodib

CE3F4

Glofitamab

Ro24-7429

Seclidemstat

AI-10-47

CE3F4 (Standard)

Epidermal Growth Factor Receptor Peptide (985-996)

[Ser25] Protein Kinase C (19-31)

Cadherin Peptide, avian

Publications Citing Use of MedChemExpress (MCE) AI-10-49

Alle RUNX Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

AI-10-49 Related Classifications

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