Kemptide
Based on 1 publication(s) in Google Scholar
Kemptide is a synthetic heptapeptide that acts as a specific substrate for cAMP-dependent protein kinase (PKA).
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- Reinheit: 99.56%
- CAS. Nr.: 65189-71-1
- Formel: C32H61N13O9
- Molecular Weight:771.91
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Speicherung:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Kemptide
More
Biologische Aktivität
Kemptide is a synthetic construct of a substrate for cAMP-dependent protein kinase (PK). Different types of intact cells catalyze the phosphorylation of Kemptide in the presence of extracellular ATP and cAMP with Km values of 3-4 uM for Kemptide[1]. Kemptide is a synthetic peptide substrate derived from pyruvate kinase[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 65189-71-1
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Appearance Solid
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Molecular Weight 771.91
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Formel C32H61N13O9
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Color White to off-white
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Sequence
Leu-Arg-Arg-Ala-Ser-Leu-Gly
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Sequence Shortening
LRRASLG
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
Lösungsmittel & Löslichkeit
H2O : ≥ 100 mg/mL (129.55 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (129.55 mM); Clear solution; Need ultrasonic
Protokoll
PKA activity is measured in pools of 10 oocytes at various stages of maturation using the SignaTECT cAMP-Dependent Protein Kinase (PKA) Assay System. Measurements of basal PKA activity are performed in the absence of exogenous cAMP, whereas measurements of total (cAMP-stimulated) PKA activity are performed in reaction buffer containing 0.025 mM cAMP[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Kübler D, et al. Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. J Biol Chem. 1989 Aug 25;264(24):14549-55. [Content Brief]
[2]. Duncan FE, et al. Transducin-like enhancer of split-6 (TLE6) is a substrate of protein kinase A activity during mouse oocyte maturation. Biol Reprod. 2014 Mar 20;90(3):63. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.2955 mL | 6.4774 mL | 12.9549 mL | 32.3872 mL |
| 5 mM | 0.2591 mL | 1.2955 mL | 2.5910 mL | 6.4774 mL | |
| 10 mM | 0.1295 mL | 0.6477 mL | 1.2955 mL | 3.2387 mL | |
| 15 mM | 0.0864 mL | 0.4318 mL | 0.8637 mL | 2.1591 mL | |
| 20 mM | 0.0648 mL | 0.3239 mL | 0.6477 mL | 1.6194 mL | |
| 25 mM | 0.0518 mL | 0.2591 mL | 0.5182 mL | 1.2955 mL | |
| 30 mM | 0.0432 mL | 0.2159 mL | 0.4318 mL | 1.0796 mL | |
| 40 mM | 0.0324 mL | 0.1619 mL | 0.3239 mL | 0.8097 mL | |
| 50 mM | 0.0259 mL | 0.1295 mL | 0.2591 mL | 0.6477 mL | |
| 60 mM | 0.0216 mL | 0.1080 mL | 0.2159 mL | 0.5398 mL | |
| 80 mM | 0.0162 mL | 0.0810 mL | 0.1619 mL | 0.4048 mL | |
| 100 mM | 0.0130 mL | 0.0648 mL | 0.1295 mL | 0.3239 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Kemptide Related Classifications
HY-P0248 Related Classifications
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