Neurotensin
Based on 5 publication(s) in Google Scholar
Neurotensin, a gut tridecapeptide, acts as a potent cellular mitogen for various colorectal and pancreatic cancers which possess high-affinity neurotensin receptors (NTR).
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- Reinheit: 99.47%
- CAS. Nr.: 39379-15-2
- Formel: C78H121N21O20
- Molecular Weight:1672.92
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Speicherung:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Neurotensin
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Biologische Aktivität
Neurotensin receptors (NTR)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
4.9 x 10-10 M
Compound: Neurotensin
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Displacement of [125I]Tyr3-neurotensin from human recombinant NT1 receptor expressed in CHO cells
Displacement of [125I]Tyr3-neurotensin from human recombinant NT1 receptor expressed in CHO cells
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[PMID: 26988801] |
| CHO | IC50 |
4.9 x 10-10 M
Compound: Neurotensin
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Displacement of [125I]Tyr3-neurotensin from human recombinant NTS1 receptor expressed in CHO cells measured after 60 mins by scintillation counting method
Displacement of [125I]Tyr3-neurotensin from human recombinant NTS1 receptor expressed in CHO cells measured after 60 mins by scintillation counting method
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[PMID: 27876250] |
| CHO-K1 | EC50 |
0.31 μM
Compound: neurotensin, NT
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Agonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as increase in intracellular calcium level by FLIPR assay
Agonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as increase in intracellular calcium level by FLIPR assay
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[PMID: 18849166] |
| CHO-K1 | IC50 |
6.03 nM
Compound: neurotensin, NT
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Antagonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as inhibition of intracellular calcium elevation pretreated 30 mins before with SR-48692 by FLIPR assay
Antagonist activity at human NTS1 receptor expressed in CHOK1 cells assessed as inhibition of intracellular calcium elevation pretreated 30 mins before with SR-48692 by FLIPR assay
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[PMID: 18849166] |
| CHO-K1 | EC50 |
0.23 nM
Compound: Neurotensin, NT
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Agonist activity at NTR1 expressed in CHOK1 cells by calcium mobilization assay
Agonist activity at NTR1 expressed in CHOK1 cells by calcium mobilization assay
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[PMID: 19195889] |
| CHO-K1 | EC50 |
0.04 nM
Compound: NT
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Agonist activity at rat NTS1 stably expressed in CHOK1 cells assessed as induction of calcium release by FLIPR assay
Agonist activity at rat NTS1 stably expressed in CHOK1 cells assessed as induction of calcium release by FLIPR assay
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[PMID: 24856674] |
| CHO-K1 | IC50 |
18.5 nM
Compound: NT
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Antagonist activity at rat NTS2 stably expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay
Antagonist activity at rat NTS2 stably expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay
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[PMID: 24856674] |
| CHO-K1 | IC50 |
114 nM
Compound: NT
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Antagonist activity at rat NTS2 receptor expressed in CHO-K1 cells assessed as inhibition of SR142948a-induced calcium mobilization by FLIPR assay
Antagonist activity at rat NTS2 receptor expressed in CHO-K1 cells assessed as inhibition of SR142948a-induced calcium mobilization by FLIPR assay
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[PMID: 25157640] |
| CHO-K1 | EC50 |
>100 nM
Compound: NT
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Agonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as increase in calcium release by FLIPR assay
Agonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as increase in calcium release by FLIPR assay
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[PMID: 25499438] |
| CHO-K1 | IC50 |
42 nM
Compound: NT
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Antagonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay
Antagonist activity at rat neurotensin receptor type 2 expressed in CHOK1 cells assessed as inhibition of SR142948a-induced calcium release by FLIPR assay
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[PMID: 25499438] |
| HEK293 | EC50 |
1.6 nM
Compound: Neurotensin
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Agonist activity at human NTS1 receptor expressed in HEK293 cells assessed as inhibition of constitutive activity on MAPK-mediated luciferase activity
Agonist activity at human NTS1 receptor expressed in HEK293 cells assessed as inhibition of constitutive activity on MAPK-mediated luciferase activity
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[PMID: 21446649] |
| HEK293 | EC50 |
110 nM
Compound: Neurotensin
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Agonist activity at human NTS2 receptor expressed in HEK293 cells assessed as inhibition of constitutive activity on MAPK-mediated luciferase activity
Agonist activity at human NTS2 receptor expressed in HEK293 cells assessed as inhibition of constitutive activity on MAPK-mediated luciferase activity
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[PMID: 21446649] |
| HT-29 | IC50 |
0.2 nM
Compound: 1
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Binding affinity towards neurotensin receptor in membranes prepared from HT-29 cell line, relative to [111In]-labeled neurotensin peptide
Binding affinity towards neurotensin receptor in membranes prepared from HT-29 cell line, relative to [111In]-labeled neurotensin peptide
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[PMID: 12852770] |
| HT-29 | IC50 |
0.62 nM
Compound: NT
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Displacement of [125I]Tyr3-NT from human NTS1R expressed in HT29 cells
Displacement of [125I]Tyr3-NT from human NTS1R expressed in HT29 cells
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[PMID: 16854083] |
| HT-29 | EC50 |
1.98 nM
Compound: NT
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Agonist activity at NTR1 in human HT-29 cells assessed as increase in intracellular calcium concentration by FLIPR assay
Agonist activity at NTR1 in human HT-29 cells assessed as increase in intracellular calcium concentration by FLIPR assay
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[PMID: 19290594] |
| N1E-115 | EC50 |
2 nM
Compound: NT
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Agonist activity at NTR1 in mouse N1E-115 cells assessed as increase in intracellular calcium concentration
Agonist activity at NTR1 in mouse N1E-115 cells assessed as increase in intracellular calcium concentration
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[PMID: 19290594] |
| WiDr | IC50 |
0.46 nM
Compound: NT
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Displacement of [125I]Tyr3-NT from human NTS1R expressed in WiDr cells
Displacement of [125I]Tyr3-NT from human NTS1R expressed in WiDr cells
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[PMID: 16854083] |
Neurotensin induces the expression of MIP-2, MCP-1, IL-1β and TNFα in murine microglial cells and stimulates IL-8 secretion in a non-transformed colon epithelial cell line stably transfected with the NTR. The high-affinity NTR, a member of the G-protein coupled receptor (GPCR) family, is present in a majority of human pancreatic and colorectal cancers, suggesting that Neurotensin (NT) may act in an endocrine fashion to affect tumor growth. Acting through the NTR1, Neurotensin is known to stimulate various signal transduction pathways, including intracellular calcium ([Ca2+]i), the mitogen-activated protein kinases (MAPKs), ERK and JNK, and various PKC isoforms. Treatment of HCT116 cells with Neurotensin (100 nM) significantly increases HCT116 cell migration (~3-fold) compared with vehicle treatment; pretreatment with Curcumin (10 μM) blocksthe stimulatory effect of NT on HCT116 cell migration. ctivation of MEK/ERK by NT and downstream induction of AP-1 transcription factors contributes to the proliferative effects of Neurotensin[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS. Nr. 39379-15-2
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Appearance Solid
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Molecular Weight 1672.92
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Formel C78H121N21O20
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Color White to off-white
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Sequence
{Pyr}-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
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Sequence Shortening
{Pyr}-LYENKPRRPYIL
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Talanta
A robust polymetallic-coated sheathless interface with high acid and alkali resistance for coupling capillary electrophoresis with mass spectrometry. [Abstract]2025 Jan 1:282:127045. PMID: 39418980 -
ACS Pharmacol Transl Sci
Critical Residues in the Gβ Subunit Mediate Selective Recruitment of the Gαq Heterotrimer to Activated G Protein-Coupled Receptors. [Abstract]2025 Jun 6;8(7):2048-2060. PMID: 40672669 -
Exp Dermatol
2024 Jan;33(1):e14990. PMID: 38071436 -
J Am Soc Mass Spectrom
Wire Desorption Combined with Electrospray Ionization Mass Spectrometry: Direct Analysis of Small Organic and Large Biological Compounds. [Abstract]2020 Jul 5. PMID: 32559077 -
Lösungsmittel & Löslichkeit
DMSO : 50 mg/mL (29.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 33.33 mg/mL (19.92 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 20 mg/mL (11.96 mM); Clear solution; Need ultrasonic
Reinheit & Dokumentation
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Data Sheet (280 KB)
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SDS (252 KB)
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- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
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- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 0.5978 mL | 2.9888 mL | 5.9776 mL | 14.9439 mL |
| 5 mM | 0.1196 mL | 0.5978 mL | 1.1955 mL | 2.9888 mL | |
| 10 mM | 0.0598 mL | 0.2989 mL | 0.5978 mL | 1.4944 mL | |
| 15 mM | 0.0399 mL | 0.1993 mL | 0.3985 mL | 0.9963 mL | |
| DMSO | 20 mM | 0.0299 mL | 0.1494 mL | 0.2989 mL | 0.7472 mL |
| 25 mM | 0.0239 mL | 0.1196 mL | 0.2391 mL | 0.5978 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Neurotensin Related Classifications
HY-P0234 Related Classifications
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