AqF026
Based on 1 Customer Validation
AqF026 is an aquaporin 1 (AQP1) agonist. AqF026 enhances the channel activity of human AQP1 in the Xenopus laevis oocyte system. AqF026 enhances the osmotic transport of water across the peritoneum in a mouse model of peritoneal dialysis. AqF026 can be used in studies related to ultrafiltration failure in peritoneal dialysis.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.05%
- CAS. Nr.: 1021869-59-9
- Formel: C20H19ClN2O5S
- Molecular Weight:434.89
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
AqF026 (0.1-100 μM) directly enhances human AQP1-mediated water transport in Xenopus laevis oocytes, with an EC50 of 3.3 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wild-type (Aqp1+/+) (sex-matched littermates, 8-12 weeks old); Aqp1-null (Aqp1-/-) (sex-matched littermates, 8-12 weeks old)[1]
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Dosage:0.75 μM; 1.5 μM; 7.5 μM; 15 μM; 30 μM
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Administration:i.v.; single injection 30 minutes prior to dialysis assay
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Result:Produced a dose-dependent increase in net ultrafiltration in wild-type mice, with a maximal 15% increase at 15 μM and an estimated EC50 of 4.2 μM.
Increased the initial ultrafiltration rate in wild-type mice by ~50% (32.2 μL/min at 15 μM.
Significantly increased intraperitoneal volume at 30, 60, and 90 minutes at 15 μM.
Enhanced sodium sieving in wild-type mice, with a greater dip in dialysate sodium concentration at 30 minutes (11.2 mEq/L) at 15 μM.
Had no effect on net ultrafiltration or initial ultrafiltration rates in Aqp1-null mice.
Did not alter the osmotic gradient, small solute transport (urea and glucose), AQP1 protein/mRNA expression, or AQP1 plasma membrane localization in wild-type mice.
Chemical Information
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CAS. Nr. 1021869-59-9
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Appearance Solid
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Molecular Weight 434.89
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Formel C20H19ClN2O5S
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Color White to off-white
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SMILES
O=C(C1=CC(S(=O)(NCC2=CC=CC=C2)=O)=C(C=C1NCC3=CC=CO3)Cl)OC
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (229.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.75 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Yool AJ, et al. AqF026 is a pharmacologic agonist of the water channel aquaporin-1. J Am Soc Nephrol. 2013 Jun;24(7):1045-52. [Content Brief]
[2]. Berliner JA, et al. Aquaporin-4 expression and modulation in a rat model of post-traumatic syringomyelia. Sci Rep. 2023;13(1):9662. Published 2023 Jun 14. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2994 mL | 11.4972 mL | 22.9943 mL | 57.4858 mL |
| 5 mM | 0.4599 mL | 2.2994 mL | 4.5989 mL | 11.4972 mL | |
| 10 mM | 0.2299 mL | 1.1497 mL | 2.2994 mL | 5.7486 mL | |
| 15 mM | 0.1533 mL | 0.7665 mL | 1.5330 mL | 3.8324 mL | |
| 20 mM | 0.1150 mL | 0.5749 mL | 1.1497 mL | 2.8743 mL | |
| 25 mM | 0.0920 mL | 0.4599 mL | 0.9198 mL | 2.2994 mL | |
| 30 mM | 0.0766 mL | 0.3832 mL | 0.7665 mL | 1.9162 mL | |
| 40 mM | 0.0575 mL | 0.2874 mL | 0.5749 mL | 1.4371 mL | |
| 50 mM | 0.0460 mL | 0.2299 mL | 0.4599 mL | 1.1497 mL | |
| 60 mM | 0.0383 mL | 0.1916 mL | 0.3832 mL | 0.9581 mL | |
| 80 mM | 0.0287 mL | 0.1437 mL | 0.2874 mL | 0.7186 mL | |
| 100 mM | 0.0230 mL | 0.1150 mL | 0.2299 mL | 0.5749 mL |
AqF026 Related Classifications
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