Art. -Nr.: HY-N8995

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Bruceoside A

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Bruceoside A, an abundant quassinoid glycoside in Fructus Bruceae, possesses anti-cancer activity.

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Reinheit: 99.27%
CAS. Nr.: 63306-30-9
Formel: C₃₂H₄₂O₁₆
Molecular Weight:682.67

Speicherung:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Bruceoside A Chemical Structure

CAS. Nr.: 63306-30-9

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Bruceoside A, an abundant quassinoid glycoside in Fructus Bruceae, possesses anti-cancer activity^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>10 μM Compound: 7	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 22506620]
BGC-823	IC₅₀	>10 μM Compound: 7	Cytotoxicity against human BGC823 cells by MTT assay Cytotoxicity against human BGC823 cells by MTT assay	[PMID: 22506620]
H9	EC₅₀	29 μM Compound: 2 (Bruceoside-A)	Inhibition of HIV-1 replication in H9 lymphocytic cells. Inhibition of HIV-1 replication in H9 lymphocytic cells.	10.1016/0960-894X(96)00096-0
H9	IC₅₀	29 μM Compound: 2 (Bruceoside-A)	Cytotoxicity against H9 lymphocytic cells. Cytotoxicity against H9 lymphocytic cells.	10.1016/0960-894X(96)00096-0
HCT-8	IC₅₀	>10 μM Compound: 7	Cytotoxicity against human HCT8 cells by MTT assay Cytotoxicity against human HCT8 cells by MTT assay	[PMID: 22506620]
HepG2	IC₅₀	>10 μM Compound: 7	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 22506620]
MCF7	IC₅₀	>50 μM Compound: 9g	Cytotoxicity against human MCF-7 cells incubated for 72 hrs by MTT assay Cytotoxicity against human MCF-7 cells incubated for 72 hrs by MTT assay	[PMID: 33289552]
MDA-MB-231	IC₅₀	>50 μM Compound: 9g	Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay	[PMID: 33289552]
SK-OV-3	IC₅₀	>10 μM Compound: 7	Cytotoxicity against human SKOV3 cells by MTT assay Cytotoxicity against human SKOV3 cells by MTT assay	[PMID: 22506620]

In Vivo

Bruceoside A could be transformed into the potent anticancer component brusatol in vivo, rather than its direct deglycosylated metabolite bruceosin^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male ICR mice weighing 25.0 ± 5.0 g^[1].
Dosage:

22.5 mg/kg.
Administration:

Intragastrically administrated.
Result:

Reached its highest levels in three hours with a half-life value of 3.99 h.

Chemical Information

CAS. Nr. 63306-30-9
Appearance Solid
Molecular Weight 682.67
Formel C₃₂H₄₂O₁₆
Color White to off-white
SMILES

O=C([C@@]1([C@H]2O)[C@@]([C@H]3OC(/C=C(C)/C)=O)([H])[C@@]([C@@](OC3=O)([H])C[C@@]4([H])[C@@H](C5=O)C)(CO1)[C@@]([C@H]2O)([H])[C@]4(C=C5O[C@@H]([C@@H]([C@H]6O)O)O[C@@H]([C@H]6O)CO)C)OC
Structure Classification
- Others
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 250 mg/mL (366.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.4648 mL

7.3242 mL

14.6484 mL

5 mM

0.2930 mL

1.4648 mL

2.9297 mL

10 mM

0.1465 mL

0.7324 mL

1.4648 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (3.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (3.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Yuan Xu, et al. Pharmacokinetic study on bruceoside A revealed the potential role of quassinoid glycosides for the anticancer properties of Fructus Bruceae. J Pharm Biomed Anal. 2019 Jun 5;170:264-272. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.4648 mL	7.3242 mL	14.6484 mL	36.6209 mL
	5 mM	0.2930 mL	1.4648 mL	2.9297 mL	7.3242 mL
	10 mM	0.1465 mL	0.7324 mL	1.4648 mL	3.6621 mL
	15 mM	0.0977 mL	0.4883 mL	0.9766 mL	2.4414 mL
	20 mM	0.0732 mL	0.3662 mL	0.7324 mL	1.8310 mL
	25 mM	0.0586 mL	0.2930 mL	0.5859 mL	1.4648 mL
	30 mM	0.0488 mL	0.2441 mL	0.4883 mL	1.2207 mL
	40 mM	0.0366 mL	0.1831 mL	0.3662 mL	0.9155 mL
	50 mM	0.0293 mL	0.1465 mL	0.2930 mL	0.7324 mL
	60 mM	0.0244 mL	0.1221 mL	0.2441 mL	0.6103 mL
	80 mM	0.0183 mL	0.0916 mL	0.1831 mL	0.4578 mL
	100 mM	0.0146 mL	0.0732 mL	0.1465 mL	0.3662 mL

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Bruceoside A

Glycoside Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Heterocyclic Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Natural Product Library

Anti-Cancer Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bruceoside A

Glycoside Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Heterocyclic Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Natural Product Library

Anti-Cancer Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bruceoside A Related Classifications

HY-N8995 Related Classifications