Feruloyl esterase

Combined pretreatment of tobacco stem pulp with feruloyl esterase (10 mL; 3 h) and cellulase U0 at 20 U/mL reduces the contents of lignin and hemicellulose, decreases fiber size and DP, increases the content of fine fractions and crystallinity, weakens intermolecular hydrogen bonding, maintains moderate thermal stability, and enhances the sensitivity of the pulp to mechanical refining^[1].
Feruloyl esterase hydrolyzes a variety of methyl hydroxycinnamates and natural feruloylated substrates. For model substrates, the range of K_m values is as follows: 0.02-1.21 mM for methyl ferulate, 0.021-0.47 mM for methyl p-coumarate, 0.02-0.43 mM for methyl caffeate, and 1.20 mM for methyl sinapate.^[2]
The K_m values of feruloyl esterase toward natural feruloylated oligosaccharides and polysaccharides range from 0.004 to 2.1 mM, covering substrates such as FAXX feruloylated xylotriose, Ara-F, Ara2F 1→5 and Xyl3Ara1F. Among these substrates, the K_m value of Ara-F is 0.075 mM, that of Ara2F 1→5 is 0.26-0.84 mM, that of Xyl3Ara1F is 2.1 mM, and that of FAXX is 0.004 mM^[2].
Feruloyl esterase preferentially targets feruloylated trisaccharides, wheat arabinoxylan and sugar beet pectin, and can release specific dimeric ferulic acid from wheat bran (e.g., solubilizing approximately 36% of saponifiable 5-5′‑DiFA in wheat bran). Its combined use with xylanase further increases the release amount of ferulic acid.^[2]
Feruloyl esterase (24 h after Agrobacterium infiltration) induces cell death in leaves of Nicotiana benthamiana, activates the expression of defense-related genes, and enhances plant resistance to Pseudomonas syringae pv. tomato DC3000^[3].
Feruloyl esterase physically interacts with Gossypium hirsutum dihydroflavonol 4-reductase (GhDFR) both in vitro and in vivo, and specifically binds to the NADPH-binding domain of GhDFR^[3].
Feruloyl esterase mediates the degradation of Gossypium hirsutum dihydroflavonol 4-reductase (GhDFR) both in vitro and in leaf cells of Nicotiana benthamiana, and colocalizes with GhDFR in the cytoplasm (the signal peptide-truncated VdFAE can also localize to the nucleus)^[3].
Feruloyl esterase (5 days post-inoculation) has a secretory signal peptide that enables VdFAE to be secreted by Verticillium dahliae (V. dahliae) and translocated into root epidermal cells of Gossypium hirsutum^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.