Art. -Nr.: HY-D1300

LysoTracker Red

(Synonyms: LysoTracker Red DND-99)

18 Cited Publications

Kundenbewertung

Based on 18 publication(s) in Google Scholar

LysoTracker Red is a Red fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 577/590 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and gather on spherical organelles. It is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes.

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Reinheit: 95.0%
CAS. Nr.: 231946-72-8
Formel: C₂₀H₂₄BF₂N₅O
Molecular Weight:399.25

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

LysoTracker Red Chemical Structure

CAS. Nr.: 231946-72-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (18)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Preparation of LysoTracker Red working solution
1. Working solution preparation:
1.1 Prepare LysoTracker Red stock solution with DMSO.
1.2 Dilute storage solution to the working solution using a medium or appropriate buffer (such as PBS) . The recommended working solution is 50-100 nM;
Note: Please adjust the concentration of the LysoTracker Red working solution according to the actual situation, and use it now.
2. Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of LysoTracker Red working solution, and then incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

590

Excitation (Ex)

577

Chemical Information

CAS. Nr. 231946-72-8
Appearance Solid
Molecular Weight 399.25
Formel C₂₀H₂₄BF₂N₅O
Color Brown to dark brown
SMILES

O=C(CCC1=CC=C2C=C3C=CC(C4=CC=CN4)=[N]3[B+3]([F-])([N-]21)[F-])NCCN(C)C
Synonyms

LysoTracker Red DND-99
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (18)

All Cell Imaging/StainingIF

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Journal Impact Factor

Nat Biotechnol

Engineering bispecific exosome activators of T cells to target immune checkpoint inhibitor-resistant metastatic melanoma. [Abstract]

2026 Jan 5. PMID: 41491256

LysoTracker Red purchased from MedChemExpress. Usage Cited in: Nat Biotechnol. 2026 Jan 5. [Abstract]

Representative immunostaining images of BEAT treated tumor cells. Lysosomes were stained with LysoTracker Red (30 min), BEAT were stained with DiD (gray), and nuclear were stained with Hoechst (blue).
J Control Release

Anti-CLEC7A nanobody in situ engineering promotes amyloid-β oligomers clearance by CAR-microglia to alleviate Alzheimer's disease pathology in mice. [Abstract]

2026 Apr 10:392:114710. PMID: 41702507
J Immunother Cancer

Reinforcing cancer immunotherapy with engineered porous hollow mycobacterium tuberculosis loaded with tumor neoantigens. [Abstract]

2025 Feb 6;13(2):e010150. PMID: 39915006

LysoTracker Red purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2025 Feb 6;13(2):e010150. [Abstract]

Neoantigens labeled with FITC were co-cultured with BMDCs for 5 hours. Lysosomes were stained with LysoTracker Red (1:500; 30 min; 4℃) dye. Confocal microscopy was used to observe co-localization of the two fluorescence with cell nuclei (DAPI fluorescence).
Mater Today Bio

A bufalin and CRISPR/Cas9 ribonucleoprotein-loaded calcium lactate nanomedicine for pyroptosis/apoptosis and synergistic cancer immunotherapy. [Abstract]

2025 Nov 7:35:102527. PMID: 41322154

LysoTracker Red purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Nov 7:35:102527. [Abstract]

Endosomal escape of CLBRP in CT26 cells was observed via CLSM at 4 and 12 h posttreatment. The colocalization of EGFP-labeled CLBRP with LysoTracker Red (20 min) -stained endosomes was analyzed.
ACS Appl Mater Interfaces

Tumor-Targeted PLGA Nanospheres Enhance Therapeutic Effect of Lenvatinib in Hepatocellular Carcinoma via Photothermal and Photodynamic Therapy. [Abstract]

2025 Aug 6;17(31):44922-44938. PMID: 40689931

LysoTracker Red purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Aug 6;17(31):44922-44938. [Abstract]

CLSM images depicted the process of lysosomal escape of nanoparticles within the cells. Red represented LysoTracker Red (6-12 h), green represented the IR780-labeled nanoparticles, and yellow regions indicated the colocalization of nanoparticles with lysosomes.
Aging Cell

Aging-induced short-chain acyl-CoA dehydrogenase promotes age-related hepatic steatosis by suppressing lipophagy. [Abstract]

2024 Jun 19:e14256. PMID: 38898632

LysoTracker Red purchased from MedChemExpress. Usage Cited in: Aging Cell. 2024 Jun 19:e14256. [Abstract]

Representative immunofluorescence images showing the colocalization of lysosomes labeled with LysoTracker Red and lipid droplets labeled with the green lipid dye BODIPY (scale bar: 50 μm).
Eur J Pharm Biopharm

Enhanced photodynamic therapy of curcumin using biodegradable PLGA coated mesoporous silica nanoparticles. [Abstract]

2024 Sep 18:114503. PMID: 39303950
PLoS Pathog

Manganese is a potent inducer of lysosomal activity that inhibits de novo HBV infection. [Abstract]

2025 Jan 2;21(1):e1012800. PMID: 39746094
iScience

Intergenerational epigenetic inheritance mediated by MYS-2/MOF in the pathogenesis of Alzheimer's disease. [Abstract]

2024 Jul 25;27(8):110588. PMID: 39220410
Biochem J

Opposite effects of spermidine and GC7 in cell culture are dictated by distinct molecular targets. [Abstract]

2025 Dec 17;482(24):1973-1992. PMID: 41408852
J Med Virol

Pexophagy Triggered by Enterovirus 71 Modulates the Production of Pro-Inflammatory Cytokines Through Cholesterol Accumulation in Lysosomes. [Abstract]

2025 Sep;97(9):e70578. PMID: 40856297
ACS Chem Biol

A Novel Lysosome Targeting Chimera for Targeted Protein Degradation via Split-and-Mix Strategy. [Abstract]

2024 May 17;19(5):1161-1168. PMID: 38662199
Res Sq

2026 Jun 21.
Res Sq

2026 May 19.
bioRxiv

2026 Mar 29.
Res Sq

2025 Dec 2.
Preprints

2025 Jul 8.
Heliyon

Repurposing fluphenazine as an autophagy modulator for treating liver cancer. [Abstract]

2023 Nov 22;9(12):e22605. PMID: 38107270

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 5 mg/mL (12.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5047 mL

12.5235 mL

25.0470 mL

5 mM

0.5009 mL

2.5047 mL

5.0094 mL

10 mM

0.2505 mL

1.2523 mL

2.5047 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Price OT, et al. Quantitative fluorescence of 5-FU-treated fetal rat limbs using confocal laser scanning microscopy and Lysotracker Red. Cytometry A. 2003;53(1):9-21. [Content Brief]

[2]. Pereira LC, et al. An autophagic process is activated in HepG2 cells to mediate BDE-100-induced toxicity. Toxicology. 2017;376:59-65. [Content Brief]

[3]. Nagahama M, et al. Cellular vacuolation induced by Clostridium perfringens epsilon-toxin. FEBS J. 2011;278(18):3395-3407. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5047 mL	12.5235 mL	25.0470 mL	62.6174 mL
	5 mM	0.5009 mL	2.5047 mL	5.0094 mL	12.5235 mL
	10 mM	0.2505 mL	1.2523 mL	2.5047 mL	6.2617 mL

Keywords

LysoTracker Red

18 Cited Publications

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LysoTracker Red

Publications Citing Use of MedChemExpress (MCE) LysoTracker Red

Biologische Aktivität

Chemical Information

Publications (18)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

LysoTracker Red Related Classifications

HY-D1300 Related Classifications