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Art. -Nr.: HY-P991526

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Biologically Validated

M701

Kundenbewertung

Based on 1 Customer Validation

M701 is a T-cell engager bispecific humanized antibody targeting epithelial cell adhesion molecule (EpCAM) and cluster of differentiation 3 (CD3). M701 binds to EpCAM on tumor cells and CD3 on T cells, thereby linking the two cell populations to achieve targeted cytotoxicity and T cell-mediated cytotoxicity. M701 is applicable to research related to advanced epithelial solid tumors.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.05%
CAS. Nr.: 2933295-42-0
Molecular Weight:125.8 kDa

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

M701 Chemical Structure

CAS. Nr.: 2933295-42-0

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Format
- Anwendung
- Verified Bioactivity
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Isotype

Bispecific T cell engager antibody

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

CD3 & EpCAM

In Vitro

M701 (serially diluted concentrations; 1 h) binds to HCT116 colorectal carcinoma cells with a dissociation constant of 32 nM^[1].
M701 (serially diluted concentrations; 1 h) binds to human T cells with a dissociation constant of 33.7 nM^[1].
M701 (0-10 μg/mL; 30 min) dose-dependently bridges EpCAM-positive NCI-N87 gastric carcinoma cells and CD3-positive CIK cells, reaching up to 40% double-positive cells at 10 μg/mL^[1].
M701 (serially diluted concentrations; 24 h) potently redirects CIK cell lysis to EpCAM-high HCT116, NCI-N87, and KATO-III tumor cells with EC₅₀ values of 1.1, 1.8, and 8.5 ng/mL respectively, shows minimal activity against EpCAM-low SK-OV-3 and MDA-MB-231 cells, and has no activity against EpCAM-negative U87 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

EpCAM-expressing tumor cell lines (HCT116, NCI-N87, KATO-III, SK-OV-3, MDA-MB-231), U87 glioblastoma cells, cytokine-induced killer (CIK) cells
Concentration:

Serially diluted concentrations
Incubation Time:

24 h (37°C, 5% CO₂ incubator)
Result:

Potently redirected CIK cell lysis to EpCAM-high HCT116 cells with an EC₅₀ of 1.1 ng/mL.
Potently redirected CIK cell lysis to EpCAM-high NCI-N87 cells with an EC₅₀ of 1.8 ng/mL.
Potently redirected CIK cell lysis to EpCAM-high KATO-III cells with an EC₅₀ of 8.5 ng/mL.
Showed minimal cytotoxicity against EpCAM-low SK-OV-3 cells with an EC₅₀ of 58.7 ng/mL.
Showed minimal cytotoxicity against EpCAM-low MDA-MB-231 cells with an EC₅₀ of 37.8 ng/mL.
Exhibited no cytotoxicity against EpCAM-negative U87 glioblastoma cells.

In Vivo

M701 (0.1-2.5 mg/kg; i.v.; on days 0, 2, and 4) dose-dependently inhibits subcutaneous HCT116 tumor growth in NOD/SCID mice, with the 2.5 mg/kg and 0.5 mg/kg doses achieving the strongest suppression, resulting in mean day-42 tumor volumes of ≤145 mm³^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

NOD/SCID (female, 7-8 weeks old)^[1]
Dosage:

0.1 mg/kg; 0.5 mg/kg; 2.5 mg/kg
Administration:

i.v.; on days 0, 2, and 4
Result:

Recorded mean tumor volume of 828.17 mm³ at 0.1 mg/kg dose on day 42.
Recorded mean tumor volume of 145.15 mm³ at 0.5 mg/kg dose on day 42.
Recorded mean tumor volume of 112.54 mm³ at 2.5 mg/kg dose on day 42.
Showed statistically significant tumor growth inhibition at 0.5 mg/kg and 2.5 mg/kg doses compared to controls.

Gene ID

916 [NCBI] & 4072 [NCBI]

Accession

P07766 & P16422

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Format

half-IGG1/scFv-h-CH2-CH3

Anwendung

ELISA, FACS, Functional assay

Verified Bioactivity

Immobilized Human EpCAM Protein, His Tag can bind M701. The EC₅₀ for this effect is 638.8 ng/mL.
Loaded M701 on ProA biosensor, can bind CD3E-His&CD3D without tag with an affinity constant of 1.73E-08 M as determined in BLI assay.

Chemical Information

CAS. Nr. 2933295-42-0
Appearance Liquid
Molecular Weight 125.8 kDa
Color Colorless to light yellow
SMILES

N/A
Versand

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (262 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

Verweise

[1]. Liu R, et al. Development and clinical trial of M701, an Anti-EpCAM × Anti-CD3 bispecific antibody: a targeted intraperitoneal therapy for malignant ascites stemming from advanced solid tumors. Exp Hematol Oncol. 2025;14(1):136. Published 2025 Nov 22. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

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Other size

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Amount: USD 0.00

Biologische Aktivität
- Beschreibung
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Format
- Anwendung
- Biological Activity
Chemical Information
Reinheit & Dokumentation
Verweise

1/16

half-IGG1/scFv-h-CH2-CH3
1/16

Immobilized Human EpCAM Protein, His Tag can bind M701. The EC₅₀ for this effect is 638.8 ng/mL.
1/16

Loaded M701 on ProA biosensor, can bind CD3E-His&CD3D without tag with an affinity constant of 1.73E-08 M as determined in BLI assay.
1/16

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M701

Cytotoxicity LDH Assay Kit

PBS Buffer (1×)

DPBS Buffer (1×)

Antibody Inhibitor Library

Biologische Aktivität

Chemical Information

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

M701 Related Classifications

HY-P991526 Related Classifications