Art. -Nr.: HY-N7696

Physalin F

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Physalin F is a secosteroid with potent anti-inflammatory and immunomodulatory activities. Physalin F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused by Human T-lymphotropic virus type 1 (HTLV-1) infection.

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Reinheit: 99.98%
CAS. Nr.: 57423-71-9
Formel: C₂₈H₃₀O₁₀
Molecular Weight:526.53

Speicherung:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Physalin F Chemical Structure

CAS. Nr.: 57423-71-9

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>50 μM Compound: 22	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
HepG2	IC₅₀	>50 μM Compound: 22	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
HT-1080	IC₅₀	2.41 μM Compound: Physalin F	Antiproliferative activity against human HT-1080 cells assessed as reduction in cell proliferation measured after 48 hrs by CCK8 assay Antiproliferative activity against human HT-1080 cells assessed as reduction in cell proliferation measured after 48 hrs by CCK8 assay	[PMID: 33735687]
L02	IC₅₀	>50 μM Compound: 22	Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
MCF7	IC₅₀	>50 μM Compound: 22	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
MCF7	IC₅₀	0.6 μM Compound: 8	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]
NCI-H460	IC₅₀	1 μM Compound: 8	Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]
PC-3	IC₅₀	1 μM Compound: 8	Cytotoxicity against human PC-3 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human PC-3 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]
RAW264.7	IC₅₀	>50 μM Compound: 22	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
RAW264.7	IC₅₀	7.9 μM Compound: 22	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-stimulated NO production pretreated with LPS followed by compund addition by Griess reagent based microplate reader assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-stimulated NO production pretreated with LPS followed by compund addition by Griess reagent based microplate reader assay	[PMID: 35608269]
SF-268	IC₅₀	0.5 μM Compound: 8	Cytotoxicity against human SF-268 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human SF-268 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]
SGC-7901	IC₅₀	>50 μM Compound: 22	Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 35608269]
WI-38	IC₅₀	3.5 μM Compound: 8	Cytotoxicity against human WI-38 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay Cytotoxicity against human WI-38 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay	[PMID: 33586438]

In Vitro

Physalin F is a seco-steroid from Physalis angulate L.^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 57423-71-9
Appearance Solid
Molecular Weight 526.53
Formel C₂₈H₃₀O₁₀
Color White to off-white
SMILES

C[C@@]([C@@](OC1=O)([H])C2)(OC3=O)[C@@]([C@@]3(CC[C@@]4([H])[C@@]5([H])C[C@@H](O6)[C@]6(CC=C7)[C@@]4(C7=O)C)O)(O[C@@]58C9=O)[C@]9([H])[C@]2([C@@]1([H])CO8)C
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (189.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8992 mL

9.4961 mL

18.9923 mL

5 mM

0.3798 mL

1.8992 mL

3.7985 mL

10 mM

0.1899 mL

0.9496 mL

1.8992 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.11 mg/mL (2.11 mM); Clear solution

This protocol yields a clear solution of ≥ 1.11 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (1.90 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Pinto LA, et al. Physalin F, a seco-steroid from Physalis angulata L., has immunosuppressive activity in peripheral blood mononuclear cells from patients with HTLV1-associated myelopathy. Biomed Pharmacother. 2016;79:129-134. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8992 mL	9.4961 mL	18.9923 mL	47.4807 mL
	5 mM	0.3798 mL	1.8992 mL	3.7985 mL	9.4961 mL
	10 mM	0.1899 mL	0.9496 mL	1.8992 mL	4.7481 mL
	15 mM	0.1266 mL	0.6331 mL	1.2662 mL	3.1654 mL
	20 mM	0.0950 mL	0.4748 mL	0.9496 mL	2.3740 mL
	25 mM	0.0760 mL	0.3798 mL	0.7597 mL	1.8992 mL
	30 mM	0.0633 mL	0.3165 mL	0.6331 mL	1.5827 mL
	40 mM	0.0475 mL	0.2374 mL	0.4748 mL	1.1870 mL
	50 mM	0.0380 mL	0.1899 mL	0.3798 mL	0.9496 mL
	60 mM	0.0317 mL	0.1583 mL	0.3165 mL	0.7913 mL
	80 mM	0.0237 mL	0.1187 mL	0.2374 mL	0.5935 mL
	100 mM	0.0190 mL	0.0950 mL	0.1899 mL	0.4748 mL

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Physalin F

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Natural Product Library

Covalent Screening Library

Traditional Chinese Medicine Active Compound Library

Food-Sourced Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Physalin F

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Natural Product Library

Covalent Screening Library

Traditional Chinese Medicine Active Compound Library

Food-Sourced Compound Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Metabolite Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Physalin F Related Classifications

Art. -Nr.: HY-N7696