RepSox hydrochloride

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 Nov;45(11):2290-2299. [Abstract]

Customer Validation
Schemes of generating Fsp1-Cre:R26R-loxp-tdTomato mice and drug treatment to induce in situ transdifferentiation. Fsp1-Cre mice were crossed with R26Rloxp-tdTomato mice in which the expression of tdTomato is prevented by a loxP-flanked STOP cassette. The F1 mice would have the red fluorescent protein tdTomato expressed specifically in the fibroblasts. CRFVPTM were given once a week for 6 weeks. Immunofluorescence staining of the cryosections of the hearts from Fsp1-Cre:R26R-loxp-tdTomato mice treated with vehicle (b) or CRFVPTM (c) for 6 weeks. C: CHIR99021, 14 mg/kg; R: RepSox, 8.6 mg/kg; F: Forskolin, 61.6 mg/kg; V: VPA, 250 mg/kg; T: TTNPB, 1 mg/kg and M: Rolipram, 2.5 mg/kg; P: Parnate, 2.7 mg/kg, CRFTM by oral gavage and VP by intraperitoneal injection once a week for 6 weeks in mice at about 8 weeks of age.

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 Nov;45(11):2290-2299. [Abstract]

Customer Validation
Whole-heart imaging with light-sheet fluorescence microscopy after tissue clearing of the hearts from Fsp1-Cre:R26R-loxp-tdTomato mice treated with vehicle (a) or CRFVPTM (b). (C: CHIR99021, 14 mg/kg; R: RepSox, 8.6 mg/kg; F: Forskolin, 61.6 mg/kg; V: VPA, 250 mg/kg; T: TTNPB, 1 mg/kg and M: Rolipram, 2.5 mg/kg; P: Parnate, 2.7 mg/kg, CRFTM by oral gavage and VP by intraperitoneal injection once a week for 6 weeks in mice at about 8 weeks of age).

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 Nov;45(11):2290-2299. [Abstract]

Customer Validation
a Schemes of obtaining PDGFRα-DreER:R26R-rox-tdTomato mice and drug treatment to induce in situ cardiac reprogramming in PDGFRα-DreER:R26R-roxtdTomato mice. PDGFRα-DreER mice were mated with R26R-rox-tdTomato mice in which the expression of tdTomato is prevented by a roxflanked STOP cassette. The expression of tdTomato in fibroblasts was turned on by tamoxifen treatment. CRFVPTM were given once a week for 6 weeks. b Body weigh change of the mice after chemical treatment. Immunofluorescence staining of the cryosections of the hearts from PDGFRα-DreER:R26R-rox-tdTomato mice treated with vehicle (c) or CRFVPTM (d) for 6 weeks. (C: CHIR99021, 14 mg/kg; R: RepSox, 8.6 mg/kg; F: Forskolin, 61.6 mg/kg; V: VPA, 250 mg/kg; T: TTNPB, 1 mg/kg and M: Rolipram, 2.5 mg/kg; P: Parnate, 2.7 mg/kg, CRFTM by oral gavage and VP by intraperitoneal injection once a week for 6 weeks in mice at about 8 weeks of age).

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 Nov;45(11):2290-2299. [Abstract]

Customer Validation
Immunofluorescence staining of α-actinin in cryosections of hearts from Fsp1-Cre:R26R-loxp-tdTomato mice treated with 7 C cocktail (CRFVPTM) or cocktails by removing 1 compound from 7 C (7C-X) for 6 weeks in vivo with Fsp1-Cre:R26R-loxp-tdTomato mice. (C: CHIR99021, 14 mg/kg; R: RepSox, 8.6 mg/kg; F: Forskolin, 61.6 mg/kg; V: VPA, 250 mg/kg; T: TTNPB, 1 mg/kg and M: Rolipram, 2.5 mg/kg; P: Parnate, 2.7 mg/kg, CRFTM by oral gavage and VP by intraperitoneal injection once a week for 6 weeks in mice at about 8 weeks of age).

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Sci Adv. 2021 Apr 14;7(16):eabb2213. [Abstract]

Customer Validation
Western blotting of the control hESC and the RCBB (including 5 μM Repsox, 6-8 d)-induced cells.

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2020 Nov 1;7(1):52. [Abstract]

Customer Validation
Chemical induction of human lung fetal IMR-90 fibroblasts into neuron-like cells. Representative microscopy images depicting the morphological changes after Repsox (2  μmol/L, 14 d) induction. Scale bars=100 μm.

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Biomaterials. 2019 Feb:193:30-46. [Abstract]

Customer Validation
Generation of RepSox (10 μM, 6 d) induced skeletal muscle like cells (CiSMLC) from mouse embryonic fibroblasts (MEFs). Immunohistochemical detection of CiSMLC with various skeletal muscle markers: MHC (MF20) and MyoD (Upper panel), α-actinin and its overlap with phase contrast image (middle panel), and Myogenin and its overlap with phase contrast images (lower panel). Magnified images in the inset are used to depict the detailed localization and co-expression and of these markers. All scale bars: 100 μm.

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Biomaterials. 2019 Feb:193:30-46. [Abstract]

Customer Validation
Generation of RepSox (10 μM, 6 d) induced skeletal muscle like cells (CiSMLC) from mouse embryonic fibroblasts (MEFs). Semi-quantitative RT-PCR analysis of various skeletal specific markers.

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Biomaterials. 2019 Feb:193:30-46. [Abstract]

Customer Validation
Representative flow cytometry analysis of α-actinin positive cells upon 4-factor (including RepSox 10 μM) treatment for indicated times (6-15 d).

RepSox hydrochloride purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Jun 19;8(36):60159-60172. [Abstract]

Customer Validation
SH-SY5Y (A) and HEK293 cells (B) are treated with 25 μM RepSox (Rep) in absence or presence of dPA or dPPA for 72 h, and the Western blotting results show that dPA or dPPA increases the expression in the presence of RepSox. RepSox does not cause significant alteration of furin protein compared to control. The expression of furin remains significantly increased after dPA or dPPA in the presence of RepSox.