Art. -Nr.: HY-117737A

RRD-251

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RRD-251 is an inhibitor of retinoblastoma tumor suppressor protein (Rb)-Raf-1 interaction, with potent anti-proliferative, anti-angiogenic and anti-tumor activities.

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Reinheit: 99.87%
CAS. Nr.: 72214-67-6
Formel: C₈H₉Cl₃N₂S
Molecular Weight:271.59

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

RRD-251 Chemical Structure

CAS. Nr.: 72214-67-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

RRD-251 is an inhibitor of retinoblastoma tumor suppressor protein (Rb)-Raf-1 interaction, with potent anti-proliferative, anti-angiogenic and anti-tumor activities^[1].

IC₅₀ & Target

Rb-Raf-1 interaction, apoptosis^[1]

Alle Raf Isoform-spezifische Produkte anzeigen :

In Vitro

RRD-251 (10-50 μM; 24 hours) inhibits melanoma growth in-vitro^[1].
RRD-251 (50 μM; 2 hours) inhibits Rb-Raf-1 interaction and Rb phosphorylation in non-small cell lung cancer cells^[1].
RRD-251 induces apoptosis (50 μM; 18 hours) and cell cycle arrest (20-50 μM; 4 hours)^[1].
RRD-251 alters the expression of cell cycle and apoptosis regulatory protein^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

SK-MEL-28 cells , SK-MEL-5 cells, SK-MEL-2 cells
Concentration:

10 μM, 20 μM, 50 μM
Incubation Time:

24 hours
Result:

Inhibited melanoma growth in-vitro.

Western Blot Analysis^[1]

Cell Line:

SK-MEL-28 cells , SK-MEL-5 cells, SK-MEL-2 cells
Concentration:

50 μM
Incubation Time:

2 hours
Result:

Showed the depletion of phosphorylated-Rb in RRD-251 treated cells.

Apoptosis Analysis^[1]

Cell Line:

SK-MEL-28 cells , SK-MEL-5 cells, SK-MEL-2 cells
Concentration:

50 μM
Incubation Time:

18 hours
Result:

Induced apoptosis.

Cell Cycle Analysis^[1]

Cell Line:

SK-MEL-28 cells , SK-MEL-5 cells
Concentration:

20 μM, 50 μM
Incubation Time:

4 hours
Result:

Resulted in a dose dependent inhibition of cell cycle progression in SK-MEL-28 and SK-MEL-5 cells, respectively.

In Vivo

RRD-251 (50 mg/kg; i.p.; q.o.d; for 14 days) has anti-cancer activities in vivo on melanomas^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

8-wk-old female athymic nude mice, with SK-ME-28 xenograft^[1]
Dosage:

50 mg/kg
Administration:

Intraperitoneal administration, qod, for 14 days
Result:

Inhibits the growth of SK-ME-28 xenograft in nude mice.

Chemical Information

CAS. Nr. 72214-67-6
Appearance Solid
Molecular Weight 271.59
Formel C₈H₉Cl₃N₂S
Color White to off-white
SMILES

NC(SCC1=CC=C(Cl)C=C1Cl)=N.[H]Cl
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (460.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6820 mL

18.4101 mL

36.8202 mL

5 mM

0.7364 mL

3.6820 mL

7.3640 mL

10 mM

0.3682 mL

1.8410 mL

3.6820 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Sandeep Singh, et al. Rb-Raf-1 interaction disruptor RRD-251 induces apoptosis in metastatic melanoma cells and synergizes with dacarbazine. Mol Cancer Ther. 2010 Dec; 9(12): 3330–3341. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6820 mL	18.4101 mL	36.8202 mL	92.0505 mL
	5 mM	0.7364 mL	3.6820 mL	7.3640 mL	18.4101 mL
	10 mM	0.3682 mL	1.8410 mL	3.6820 mL	9.2051 mL
	15 mM	0.2455 mL	1.2273 mL	2.4547 mL	6.1367 mL
	20 mM	0.1841 mL	0.9205 mL	1.8410 mL	4.6025 mL
	25 mM	0.1473 mL	0.7364 mL	1.4728 mL	3.6820 mL
	30 mM	0.1227 mL	0.6137 mL	1.2273 mL	3.0684 mL
	40 mM	0.0921 mL	0.4603 mL	0.9205 mL	2.3013 mL
	50 mM	0.0736 mL	0.3682 mL	0.7364 mL	1.8410 mL
	60 mM	0.0614 mL	0.3068 mL	0.6137 mL	1.5342 mL
	80 mM	0.0460 mL	0.2301 mL	0.4603 mL	1.1506 mL
	100 mM	0.0368 mL	0.1841 mL	0.3682 mL	0.9205 mL

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RRD-251

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Anti-Cancer Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Glutamine Metabolism Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Obesity Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Membrane Receptor-targeted Compound Library

Cell Death Library

Serine/Threonine Kinase Inhibitor Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Alle Raf Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

RRD-251

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Anti-Cancer Compound Library

Oxygen Sensing Compound Library

Ferroptosis Compound Library

Glutamine Metabolism Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Obesity Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Membrane Receptor-targeted Compound Library

Cell Death Library

Serine/Threonine Kinase Inhibitor Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Alle Raf Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

RRD-251 Related Classifications

HY-117737A Related Classifications