Art. -Nr.: HY-100815B

(RS)-AMPA

Name: (RS)-AMPA
Brand: MedChemExpress
SKU: HY-100815B
Price: 180.00 USD
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(Synonyms: (±)-AMPA)

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(RS)-AMPA ((±)-AMPA) is a glutamate analogue and a potent and selective excitatory neurotransmitter L-glutamic acid agonist. (RS)-AMPA does not interfere with binding sites for kainic acid or NMDA receptors.

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Reinheit: 99.91%
CAS. Nr.: 77521-29-0
Formel: C₇H₁₀N₂O₄
Molecular Weight:186.17

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

(RS)-AMPA Chemical Structure

CAS. Nr.: 77521-29-0

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle iGluR Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	16 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA4 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Agonist activity at rat recombinant GluA4 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	37 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA3 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Agonist activity at rat recombinant GluA3 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	53 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA1 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Agonist activity at rat recombinant GluA1 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	67 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA2 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Agonist activity at rat recombinant GluA2 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
Oocyte	EC₅₀	1.4 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA3 receptor flop isoform expressed in Xenopus oocytes Agonist activity at rat recombinant GluA3 receptor flop isoform expressed in Xenopus oocytes	[PMID: 20096591]
Oocyte	EC₅₀	1.4 μM Compound: 1 (AMPA)	Effective concentration of compound was determined for electrophysiological properties on Ionotropic glutamate receptor AMPA 3 (GluR3) using rat cortical slice model in Xenopus laevis oocytes Effective concentration of compound was determined for electrophysiological properties on Ionotropic glutamate receptor AMPA 3 (GluR3) using rat cortical slice model in Xenopus laevis oocytes	[PMID: 12747796]
Oocyte	EC₅₀	3.31 μM Compound: (R,S)-AMPA	Agonist activity at recombinant GluA1A2 receptor flip isoform expressed in Xenopus oocytes Agonist activity at recombinant GluA1A2 receptor flip isoform expressed in Xenopus oocytes	[PMID: 20096591]
Oocyte	EC₅₀	5.01 μM Compound: (R,S)-AMPA	Agonist activity at recombinant GluA2A4 receptor flip isoform expressed in Xenopus oocytes Agonist activity at recombinant GluA2A4 receptor flip isoform expressed in Xenopus oocytes	[PMID: 20096591]
Oocyte	EC₅₀	8.7 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA1 receptor flop isoform expressed in Xenopus oocytes Agonist activity at rat recombinant GluA1 receptor flop isoform expressed in Xenopus oocytes	[PMID: 20096591]
Oocyte	EC₅₀	8.7 μM Compound: 1 (AMPA)	Effective concentration of compound was determined for electrophysiological properties on Ionotropic glutamate receptor AMPA 1 using rat cortical slice model in Xenopus laevis oocytes Effective concentration of compound was determined for electrophysiological properties on Ionotropic glutamate receptor AMPA 1 using rat cortical slice model in Xenopus laevis oocytes	[PMID: 12747796]

In Vitro

(RS)-AMPA (10-3-10-4 M) causes depolarizations of cultured rat spinal and brainstem neurones. The depolarization by (RS)-AMPA is clearly dose-dependent, although there is a great variability of effects between individual neurones. Application of (RS)-AMPA at 10-5 M produces only small depolarizations (3-7 mV), whereas at 10-4 M, the amplitudes of the depolarizations ranged from 4 to 33 mV. (RS)-AMPA also causes an increase of the discharge rate of spontaneously firing neurones or sometimes evoked a short burst of action potentials in silent cells. (RS)-AMPA exerts its depolarizing effects by activating glutamate/quisqualate receptors without affecting NMDA receptors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 77521-29-0
Appearance Solid
Molecular Weight 186.17
Formel C₇H₁₀N₂O₄
Color White to off-white
SMILES

O=C1C(CC(N)C(O)=O)=C(C)ON1
Synonyms

(±)-AMPA
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 100 mg/mL (537.14 mM; Need ultrasonic)

DMSO : 5 mg/mL (26.86 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.3714 mL

26.8572 mL

53.7143 mL

5 mM

1.0743 mL

5.3714 mL

10.7429 mL

10 mM

0.5371 mL

2.6857 mL

5.3714 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 0.5 mg/mL (2.69 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 0.5 mg/mL (2.69 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 0.5 mg/mL (2.69 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Hösli L, et al. Effects of the glutamate analogue AMPA and its interaction with antagonists on cultured rat spinal and brain stem neurones. Neurosci Lett. 1983 Mar 28;36(1):59-62. [Content Brief]

[2]. Sommer B, et al. Flip and flop: a cell-specific functional switch in glutamate-operated channels of the CNS. Science. 1990 Sep 28;249(4976):1580-5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	5.3714 mL	26.8572 mL	53.7143 mL	134.2859 mL
	5 mM	1.0743 mL	5.3714 mL	10.7429 mL	26.8572 mL
	10 mM	0.5371 mL	2.6857 mL	5.3714 mL	13.4286 mL
	15 mM	0.3581 mL	1.7905 mL	3.5810 mL	8.9524 mL
	20 mM	0.2686 mL	1.3429 mL	2.6857 mL	6.7143 mL
	25 mM	0.2149 mL	1.0743 mL	2.1486 mL	5.3714 mL
H₂O	30 mM	0.1790 mL	0.8952 mL	1.7905 mL	4.4762 mL
	40 mM	0.1343 mL	0.6714 mL	1.3429 mL	3.3571 mL
	50 mM	0.1074 mL	0.5371 mL	1.0743 mL	2.6857 mL
	60 mM	0.0895 mL	0.4476 mL	0.8952 mL	2.2381 mL
	80 mM	0.0671 mL	0.3357 mL	0.6714 mL	1.6786 mL
	100 mM	0.0537 mL	0.2686 mL	0.5371 mL	1.3429 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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(RS)-AMPA

NMDA

Kainic acid

(RS)-AMPA (Standard)

Alle iGluR Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

(RS)-AMPA

NMDA

Kainic acid

(RS)-AMPA (Standard)

Alle iGluR Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

(RS)-AMPA Related Classifications

HY-100815B Related Classifications

Bioz