Art. -Nr.: HY-119357

TN-16

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TN-16 is a Microtubule polymerization inhibitor. TN-16 induces G₂/M cell cycle arrest, metaphase mitotic arrest and Apoptotic cell death in cells, and blocks late Autophagic flux by inhibiting autophagosome-lysosome fusion. TN-16 suppresses tumor growth in syngeneic mouse breast cancer models. TN-16 can be used in research related to neuroblastoma, cervical cancer, breast cancer and other tumors.

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Reinheit: 99.78%
CAS. Nr.: 33016-12-5
Formel: C₁₉H₁₈N₂O₂
Molecular Weight:306.36

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

TN-16 Chemical Structure

CAS. Nr.: 33016-12-5

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

TN-16 (1 μM; 72 h) potently reduces the viability of neuroblastoma SH-SY5Y cells, with an EC₅₀ of 68 nM^[1].
TN-16 exhibits cytotoxicity against cervical cancer HeLa cells^[1].
TN-16 (200 nM-1 μM; 16 h) induces G₂/M phase cell cycle arrest in neuroblastoma SH-SY5Y cells^[1].
TN-16 (1 μM; 16 h) causes only an extremely slight change in the ratio of tubulin polymerization/depolymerization in neuroblastoma SH-SY5Y cells^[1].
TN-16 (1 μM-10 μM; 61 min) inhibits the polymerization of purified tubulin in a dose-dependent manner, and a strong inhibitory effect is observed at a concentration of 10 μM^[1].
TN-16 (1.25 µmol/L; 24-48 h) enhances apoptosis activation, which is manifested as increased cleavage levels of Caspase-3 and PARP in Atg7-knockdown HCT-116 (Bax^+/-) cells^[2].
Incubation with TN-16 (10 μM; up to 60 min) at 30°C for up to 60 min inhibits the assembly of porcine brain tubulin in vitro, as determined by turbidity measurement at 350 nm^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

human neuroblastoma SH-SY5Y cells
Concentration:

1 μM
Incubation Time:

72 h
Result:

Exhibited cytotoxic activity with an EC₅₀ of 68 nM.
Reduced cell viability to 34.7% of control.

Cell Cycle Analysis^[1]

Cell Line:

human neuroblastoma SH-SY5Y cells
Concentration:

200 nM-1 μM
Incubation Time:

16 h
Result:

Induced a distinct G₂/M cell cycle arrest at both tested concentrations.

Western Blot Analysis^[2]

Cell Line:

Atg7-knockdown human HCT-116 (Bax+/-) colon cancer cells
Concentration:

1.25 µmol/L
Incubation Time:

24, 48 h
Result:

Induced enhanced cleavage of caspase-3 and PARP in Atg7-knockdown cells relative to autophagy-proficient scrambled control cells.

In Vivo

TN-16 (1 mg/kg; i.p.; daily; for 11 consecutive days) significantly inhibits breast tumor growth in orthotopic mouse models, with its mechanism of action being the blockade of autophagic flux and induction of apoptosis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Crl:CD1-Foxn1nu (5- to 6-week-old female; orthotopic syngeneic breast cancer model via transplantation of 1×10⁶ 4T1 mouse mammary carcinoma cells into the mammary fat pad)^[2]
Dosage:

1 mg/kg
Administration:

i.p.; daily; 11 days
Result:

Reduced tumour volume to ~270 mm³ by day 11 post-treatment initiation versus ~520 mm³ in controls.
Lowered average harvested tumour weight significantly compared to controls.
Increased levels of cleaved PARP and cleaved caspase-3 in tumour tissue lysates.
Induced accumulation of p62 and increased LC3-II turnover, indicating blockade of autophagic flux and enhanced apoptosis.

Chemical Information

CAS. Nr. 33016-12-5
Appearance Solid
Molecular Weight 306.36
Formel C₁₉H₁₈N₂O₂
Color White to off-white
SMILES

O=C(NC(C/1=O)CC2=CC=CC=C2)C1=C(C)/NC3=CC=CC=C3
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (163.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2641 mL

16.3207 mL

32.6413 mL

5 mM

0.6528 mL

3.2641 mL

6.5283 mL

10 mM

0.3264 mL

1.6321 mL

3.2641 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (597 KB)
- English - EN (597 KB)
- Français - FR (597 KB)
- Deutsch - DE (597 KB)
- Norwegian - NO (597 KB)
- Español - ES (597 KB)
- Swedish - SV (597 KB)
- Italian - IT (597 KB)
- Korean - KR (597 KB)
- Portuguese - PT (597 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Foroutan A, et al. Identification of novel aza-analogs of TN-16 as disrupters of microtubule dynamics through a multicomponent reaction. Eur J Med Chem. 2023;245(Pt 1):114895. [Content Brief]

[2]. Hasanain M, et al. Microtubule disrupting agent-mediated inhibition of cancer cell growth is associated with blockade of autophagic flux and simultaneous induction of apoptosis. Cell Prolif. 2020;53(4):e12749. [Content Brief]

[3]. Arai T, et al. Inhibition of microtubule assembly in vitro by TN-16, a synthetic antitumor drug. FEBS Lett. 1983;155(2):273-276. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2641 mL	16.3207 mL	32.6413 mL	81.6033 mL
	5 mM	0.6528 mL	3.2641 mL	6.5283 mL	16.3207 mL
	10 mM	0.3264 mL	1.6321 mL	3.2641 mL	8.1603 mL
	15 mM	0.2176 mL	1.0880 mL	2.1761 mL	5.4402 mL
	20 mM	0.1632 mL	0.8160 mL	1.6321 mL	4.0802 mL
	25 mM	0.1306 mL	0.6528 mL	1.3057 mL	3.2641 mL
	30 mM	0.1088 mL	0.5440 mL	1.0880 mL	2.7201 mL
	40 mM	0.0816 mL	0.4080 mL	0.8160 mL	2.0401 mL
	50 mM	0.0653 mL	0.3264 mL	0.6528 mL	1.6321 mL
	60 mM	0.0544 mL	0.2720 mL	0.5440 mL	1.3601 mL
	80 mM	0.0408 mL	0.2040 mL	0.4080 mL	1.0200 mL
	100 mM	0.0326 mL	0.1632 mL	0.3264 mL	0.8160 mL

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TN-16

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Cytoskeleton Compound Library

Anti-Lung Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Cysteine Targeted Covalent Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

TN-16

Apoptosis Compound Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Cytoskeleton Compound Library

Anti-Lung Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Cysteine Targeted Covalent Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

TN-16 Related Classifications

HY-119357 Related Classifications