Ercalcidiol
Based on 2 publication(s) in Google Scholar
Ercalcidiol is a metabolite of vitamin D2, is regarded as an indicator of vitamin D nutritional status.
For research use only. We do not sell to patients.
- Purity: 99.75%
- CAS No.: 21343-40-8
- Formula: C28H44O2
- Molecular Weight:412.65
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Storage:
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Ercalcidiol
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
Differentiation between Ercalcidiol (25(OH)D2) and 25(OH)D3 is important for monitoring vitamin D therapy, as vitamin D2 is the predominant prescription form. The half-life of Ercalcidiol is shorter than that of 25(OH)D3 and it binds less well to the vitamin D binding protein, making it less potent and, therefore, required to be administered at much higher doses than vitamin D3. Some currently used assays have a diminished capacity to detect Ercalcidiol, which can lead to dangerous overdosing when attempting to monitor therapy with vitamin D2[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 21343-40-8
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Appearance Solid
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Molecular Weight 412.65
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Formula C28H44O2
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Color White to off-white
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SMILES
C=C1CC[C@H](O)C/C1=C/C=C2[C@]3([C@@](C)([C@H](CC3)[C@@H](/C=C/[C@@H](C(C)(O)C)C)C)CCC/2)[H]
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Synonyms
25-Hydroxy Vitamin D2
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (2)
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Journal Impact Factor
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Most Recent
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Sci Rep
Quantitative NMR (qNMR) spectroscopy based investigation of the absolute content, stability and isomerization of 25-hydroxyvitamin D2/D3 and 24(R),25-dihydroxyvitamin D2 in solution phase. [Abstract]2022 Feb 22;12(1):3014. PMID: 35194108 -
Virol Sin
Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo. [Abstract]2024 Oct;39(5):802-811. PMID: 39168248
Solvent & Solubility
DMSO : 100 mg/mL (242.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.06 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.06 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * The compound is unstable in solutions, freshly prepared is recommended.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (585 KB)
- English - EN (585 KB)
- Français - FR (585 KB)
- Deutsch - DE (585 KB)
- Norwegian - NO (585 KB)
- Español - ES (585 KB)
- Swedish - SV (585 KB)
- Italian - IT (585 KB)
- Korean - KR (585 KB)
- Portuguese - PT (585 KB)
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Handling Instructions (2659 KB)
References
[1]. Li L, et al. Performance evaluation of two immunoassays for 25-hydroxyvitamin D. J Clin Biochem Nutr. 2016 May;58(3):186-92. [Content Brief]
[2]. Newman MS, et al. A liquid chromatography/tandem mass spectrometry method for determination of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 in dried blood spots: a potential adjunct to diabetes and cardiometabolic risk screening. J Diabetes Sci Technol [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4234 mL | 12.1168 mL | 24.2336 mL | 60.5840 mL |
| 5 mM | 0.4847 mL | 2.4234 mL | 4.8467 mL | 12.1168 mL | |
| 10 mM | 0.2423 mL | 1.2117 mL | 2.4234 mL | 6.0584 mL | |
| 15 mM | 0.1616 mL | 0.8078 mL | 1.6156 mL | 4.0389 mL | |
| 20 mM | 0.1212 mL | 0.6058 mL | 1.2117 mL | 3.0292 mL | |
| 25 mM | 0.0969 mL | 0.4847 mL | 0.9693 mL | 2.4234 mL | |
| 30 mM | 0.0808 mL | 0.4039 mL | 0.8078 mL | 2.0195 mL | |
| 40 mM | 0.0606 mL | 0.3029 mL | 0.6058 mL | 1.5146 mL | |
| 50 mM | 0.0485 mL | 0.2423 mL | 0.4847 mL | 1.2117 mL | |
| 60 mM | 0.0404 mL | 0.2019 mL | 0.4039 mL | 1.0097 mL | |
| 80 mM | 0.0303 mL | 0.1515 mL | 0.3029 mL | 0.7573 mL | |
| 100 mM | 0.0242 mL | 0.1212 mL | 0.2423 mL | 0.6058 mL |
Ercalcidiol Related Classifications
HY-32349 Related Classifications
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