Cat. No.: HY-N0659

Jujuboside A

4 Cited Publications

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Based on 4 publication(s) in Google Scholar

Jujuboside A is a glycoside extracted from Semen Ziziphi Spinosae, a Chinese herbal medicine used to treat insomnia and anxiety.

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Pureté: 99.88%
CAS No.: 55466-04-1
Formule: C₅₈H₉₄O₂₆
Masse moléculaire:1207.35

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Jujuboside A Chemical Structure

CAS No.: 55466-04-1

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvant et solubilité
- In Vitro
- In Vivo
Protocole
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Jujuboside A is a glycoside extracted from Semen Ziziphi Spinosae, a Chinese herbal medicine used to treat insomnia and anxiety.

IC₅₀ & Target

GABA Receptor, mTOR, PI3K, Akt^[1]^[2]

In Vitro

Jujuboside A at the low dose of 41 μM (about 0.05 g/L) induces significant increase of GABA(A) receptor α1, α5, β2 subunit mRNAs in both 24 and 72h treatments. Jujuboside A at the high dose of 82 μM (about 0.1 g/L) significantly increases GABA(A) receptor α1, α5 subunit mRNA levels and decreases β2 subunit mRNA level at 24h treatment, and decreases GABA(A) receptor subunit α1, β2 mRNAs expression at 72h treatment^[1]. Jujuboside A pretreatment could reverse the reduction of cell viability and better the injury of H9C2 cells induced by ISO. Jujuboside A could accelerate the phosphorylation of PI3K, Akt, and mTOR. Jujuboside A could significantly decrease the ratio of microtubule-associated protein LC3-II/I in H9C2 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

During daytime (9:00-15:00), jujubosides significantly increases the total sleep and rapid eye movement (REM) sleep without significant influence on non-REM (NREM) sleep. During nighttime (21:00-3:00), jujubosides significantly increases the total sleep and NREM sleep especially the light sleep while shows no significant effect on REM sleep and slow wave sleep (SWS)^[3]. Intracerebroventricular treatment with Jujuboside A significantly mitigates learning and memory impairment in mice induced by Aβ1–42 as measured by the Y-maze, active avoidance and Morris water maze. Intracerebroventricular treatment with Jujuboside A reduces the level of Aβ1–42 in hippocampus, significantly inhibits the activities of acetylcholinesterase (AChE) and NO, and decreases the amount of the increased malondialdehyde (MDA) in the hippocampus and cerebral cortex of mice treated with intracerebroventricular injection of Aβ1–42^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 55466-04-1
Appearance Solid
Masse moléculaire 1207.35
Formule C₅₈H₉₄O₂₆
Color White to off-white
SMILES

C[C@]([C@]1([H])CC2)(CC[C@]3([H])[C@@]1(CC[C@H](O[C@@](OC[C@H](O)[C@@H]4O[C@@](O[C@H](CO[C@@H]([C@@H]([C@@H](O)[C@@H]5O)O)O[C@@H]5CO)[C@@H](O)[C@@H]6O)([H])[C@@H]6O[C@@](OC[C@@H](O)[C@@H]7O)([H])[C@@H]7O)([H])[C@@H]4O[C@@](O[C@@H](C)[C@H](O)[C@H]8O)([H])[C@@H]8O)C3(C)C)C)[C@@]9(CO%10)[C@@]2([H])[C@]([C@]%11(O)C)([H])[C@@]%10(O[C@@H](/C=C(C)/C)C%11)C9
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Plants
- Rhamnaceae
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

All WBCell Proliferation/Viability AssayApoptosis AnalysisIFFlow Cytometry

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Journal Impact Factor

Cancer Biol Ther

Jujuboside A induces bladder cancer cell apoptosis by inhibiting ATP1A2-mediated mitochondrial energy metabolism regulation. [Abstract]

2026 Dec 31;27(1):2615418. PMID: 41533486

Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]

T24 cells were treated with different concentrations of Jujuboside A (JuA) (12.5, 25, and 50 μmol/L) for 48 h. Changes in relative ATP1A2 protein levels were subsequently assessed via western blotting.

Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]

After treating T24 cells with different concentrations of Jujuboside A (JuA) (6.125, 12.5, 25, 50, and 100 μmol/L) for 48 h, changes in cell viability were analyzed via CCK8 assay.

Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]

After treatment with Jujuboside A (JuA) (50 μmol/L) and overexpression plasmid transfection, changes in the cell apoptosis ratio were assessed via flow cytometry with FITC/PI double staining.

Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]

After treating with 50 μmol/L of Jujuboside A (JuA) for 48 h, transfecting with the ATP1A2 overexpression plasmid, and culturing for another 48 h, changes in the mitochondrial membrane potential of T24 cells were assessed via a JC-1 mitochondrial membrane potential fluorescence probe assay.

Jujuboside A purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2026 Dec 31;27(1):2615418. [Abstract]

After treatment with Jujuboside A (JuA) (50 μmol/L) and overexpression plasmid transfection, changes in the intracellular ROS levels in T24 cells were assessed via flow cytometry.
Biochim Biophys Acta Mol Basis Dis

JuA alleviates liver ischemia-reperfusion injury by activating AKT/NRF2/HO-1 pathways. [Abstract]

2024 Sep 3:167496. PMID: 39237046
Med Oncol

FAT4 activation inhibits epithelial-mesenchymal transition (EMT) by promoting autophagy in H2228/Cer cells. [Abstract]

2022 Dec 28;40(1):64. PMID: 36576661
Research Square Preprint

2021 Nov.

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (82.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (41.41 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

0.8283 mL

4.1413 mL

8.2826 mL

5 mM

0.1657 mL

0.8283 mL

1.6565 mL

10 mM

0.0828 mL

0.4141 mL

0.8283 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL (41.41 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Protocole

Cell Assay^[1]

After 7 days in culture in vitro, cells are exposed continuously for the next 24 or 72 h to the culture medium with either Jujuboside A or diazepam, or neither of them. Vehicle is added for the control group; 10 μM diazepam is added for the diazepam group; Jujuboside A 82 μM (about 0.1 g/L) and 41 μM (about 0.05 g/L) are added for the JuA-H (high dose Jujuboside A) and the JuA-L (low dose Jujuboside A) groups respectively. Total RNA is isolated from cells for further analysis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

Mice: Cognitive impairment of mice is induced by ICV injection of Aβ1-42. Then mice are given intracerebroventricular (ICV) injection of Jujuboside A (0.02 and 0.2 mg/kg) for five consecutive days. Y-maze, active avoidance and Morris water maze tests are performed on mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (286 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. You ZL, et al. Effects on the expression of GABAA receptor subunits by jujuboside A treatment in rathippocampal neurons. J Ethnopharmacol. 2010 Mar 24;128(2):419-23. [Content Brief]

[2]. Han D, et al. Jujuboside A Protects H9C2 Cells from Isoproterenol-Induced Injury via Activating PI3K/Akt/mTOR Signaling Pathway. Evid Based Complement Alternat Med. 2016;2016:9593716. [Content Brief]

[3]. Cao JX, et al. Hypnotic effect of jujubosides from Semen Ziziphi Spinosae. J Ethnopharmacol. 2010 Jul 6;130(1):163-6. [Content Brief]

[4]. Liu Z, et al. Jujuboside A, a neuroprotective agent from semen Ziziphi Spinosae ameliorates behavioral disorders of the dementia mouse model induced by Aβ 1-42. Eur J Pharmacol. 2014 Sep 5;738:206-13. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	0.8283 mL	4.1413 mL	8.2826 mL	20.7065 mL
	5 mM	0.1657 mL	0.8283 mL	1.6565 mL	4.1413 mL
	10 mM	0.0828 mL	0.4141 mL	0.8283 mL	2.0707 mL
	15 mM	0.0552 mL	0.2761 mL	0.5522 mL	1.3804 mL
	20 mM	0.0414 mL	0.2071 mL	0.4141 mL	1.0353 mL
	25 mM	0.0331 mL	0.1657 mL	0.3313 mL	0.8283 mL
	30 mM	0.0276 mL	0.1380 mL	0.2761 mL	0.6902 mL
	40 mM	0.0207 mL	0.1035 mL	0.2071 mL	0.5177 mL
DMSO	50 mM	0.0166 mL	0.0828 mL	0.1657 mL	0.4141 mL
	60 mM	0.0138 mL	0.0690 mL	0.1380 mL	0.3451 mL
	80 mM	0.0104 mL	0.0518 mL	0.1035 mL	0.2588 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Jujuboside A

4 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Jujuboside A

Activité biologique

Chemical Information

Publications (4)

Solvant et solubilité

Protocole

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Jujuboside A Related Classifications

HY-N0659 Related Classifications