Clostripain
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Clostripain (Clostridiopeptidase B) is a thiol protease isolated from Clostridium histolyticum. Clostripain exhibits proteolytic activity as well as amidase-esterase activity. The specificity of Clostripain is primarily restricted to arginine residues, but it also shows minor hydrolytic activity toward most lysine-containing substrates. Clostripain catalyzes the ammonolysis of Carbobenzoxyarginyl methyl ester to generate various dipeptides.
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- CAS No.: 9028-00-6
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Microbial Metabolite |
Clostripain exhibits proteolytic activity as well as amidase-esterase activity. Its specificity is primarily restricted to arginine residues, but it also shows minor hydrolytic activity toward most lysine-containing substrates[1].
Clostripain catalyzes the ammonolysis of Carbobenzoxyarginyl methyl ester to produce a variety of dipeptides[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.4.22.8
≥50 U/mg soild
One unit is defined as the amount of enzyme that will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of 2.5 mM DTT.
Chemical Information
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CAS No. 9028-00-6
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Appearance Solid
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Color White to off-white
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SMILES
[Clostripain]
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Synonyms
Clostridiopeptidase B
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (266 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Mitchell WM, et al. Purification and properties of clostridiopeptidase B (Clostripain). J Biol Chem. 1968 Sep 25;243(18):4683-92. [Content Brief]
[2]. Fortier G, et al. Kinetic study of nucleophile specificity in dipeptide synthesis catalyzed by clostridiopeptidase B. Arch Biochem Biophys. 1990;276(2):317-321. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)