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Cat. No.: HY-P990569

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Biologically Validated

CM-24

Name: CM-24
Brand: MedChemExpress
SKU: HY-P990569
Price: 300.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: MK-6018)

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Based on 1 Customer Validation

CM-24 (MK-6018) is a highly selective humanized monoclonal antibody targeting CEACAM1/CD66a. CM-24 binds to the N-terminal domain of CEACAM1, blocks the CEACAM1-CEACAM1 interaction, relieves the co-inhibitory effect on CEACAM1-positive lymphocytes, and enhances the cytotoxic activity of TIL, LAK and NK cells against CEACAM1-positive tumor cells. CM-24 can be used in studies related to tumor immunology, tumor metastasis and thrombosis. For the isotype control of CM-24, refer to Human IgG1 kappa, Isotype Control (HY-P99001).

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Pureté: 96.02%
CAS No.: 1783801-93-3
Masse moléculaire:144.98 kDa

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

CM-24 Chemical Structure

CAS No.: 1783801-93-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Essai clinique
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Application
- Verified Bioactivity
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

^[1]

CEACAM1

In Vitro

CM-24 (MK-6018) (0.1-10 μg/mL; pre-treated for 30 min followed by 24 h co-culture) dose-dependently enhances the cytotoxic activity of human LAK cells against SK-MEL-28 melanoma cells^[2].
CM-24 (0.1-10 μg/mL; 30 min pre-treatment followed by 24 h co-culture) enhances the cytotoxic activity of LAK cells against T3M4, SU8686 and PANC2 pancreatic cancer cells as well as H358 and H460 lung cancer cells, and increases the secretion of Granzyme B and IFNγ^[2].
CM-24 (20 μg/mL; 1 h) specifically binds to NET structures formed by PMA (HY-18739)-stimulated primary human neutrophils, while no detectable staining is observed with the human IgG4 isotype control^[3].
CM-24 (500 μg/mL; 24-48 h) inhibits NETs (20 ng/μL)-induced migration of SK-MEL-28, A549 and AsPC-1 cells by 69.5%, 82.7% and 49.1%, respectively^[3].
CM-24 (500 μg/mL; pre-treated for 30 min followed by 2 h of incubation) significantly reduces the adhesion of CEACAM1-positive SK-MEL-28 cells to 4 ng/μL NETs^[3].
CM-24 (500 μg/mL; 30 min pre-treatment) reduces platelet aggregation induced by 5 and 10 ng/μL fresh NETs by 70% and 53%, respectively, compared with IgG4 isotype control^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Migration Assay ^[3]

Cell Line:

SK-MEL-28, A549 and AsPC-1 cells
Concentration:

CM-24: 500 μg/mL; NETs: 20 ng/μL
Incubation Time:

SK-MEL-28/A549: 24 h; AsPC-1: 48 h
Result:

Inhibited NET-induced migration by 69.5%, 82.7% and 49.1%, respectively.

Immunofluorescence^[3]

Cell Line:

NETs generated from PMA-stimulated primary human neutrophils
Concentration:

CM-24: 20 μg/mL; PMA: 100 nM
Incubation Time:

NET induction: 4 h; CM-24 staining: 1 h
Result:

Specifically bound NET structures, whereas isotype control showed no detectable staining.

In Vivo

CM-24 (MK-6018) (1-10 mg/kg; i.v.; twice weekly; for 44 consecutive days) combined with intravenously administered melanoma-reactive TIL, achieves tumor growth inhibition (TGI) rates of 84%, 87%, 90% and 93%, respectively, in the SCID-NOD mouse MEL526 melanoma lung metastasis model^[2].
Combination of CM-24 (1-10 mg/kg; i.v.; twice weekly) with melanoma-reactive TIL reduces the median number of lung lesions to 5, 6, 3, and 2, respectively, compared with 45 in the TIL plus control IgG group^[2].
CM-24 (25-100 mg/kg; intravenous infusion; once every 2 weeks; 4 doses administered over 6 weeks) causes no treatment-related toxicity, mortality or pathological changes in rhesus monkeys^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Intravenous MEL526 melanoma lung metastasis model with adoptively transferred melanoma-reactive TILs^[2]
Dosage:

1, 3, 6 and 10 mg/kg
Administration:

CM-24: i.v., twice weekly for 44 days; TILs: i.v., every 10 days
Result:

Produced TGI values of 84%, 87%, 90% and 93%, respectively; statistical significance was observed at 6 and 10 mg/kg.

Animal Model:

Intravenous MEL526 melanoma lung metastasis model^[2]
Dosage:

1, 3, 6 and 10 mg/kg
Administration:

i.v., twice weekly; combined with TILs
Result:

Reduced median lung lesion counts to 5, 6, 3 and 2, respectively, compared with 45 in the TIL plus control IgG group.

Animal Model:

TIL receptor-occupancy assessment in lung lesions^[2]
Dosage:

1, 3, 6 and 10 mg/kg
Administration:

i.v., twice weekly
Result:

Produced approximately 50% CEACAM1 receptor occupancy at 1 mg/kg and >90% occupancy at 3, 6 and 10 mg/kg.

Essai clinique

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Gene ID

634 [NCBI]

Accession

P13688

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Application

ELISA, FACS, Functional assay

Verified Bioactivity

Immobilized CEACAM1 Protein, Human can bind CM-24. The EC₅₀ for this effect is 3.38 ng/mL.
Flow cytometric analysis of 1.5X10⁶ HT-29 cells with CM-24 (HY-P990569, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Chemical Information

CAS No. 1783801-93-3
Appearance Liquid
Masse moléculaire 144.98 kDa
Color Colorless to light yellow
Synonyms

MK-6018
Livraison

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (266 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

Références

[1]. Markel G, et al. Inhibition of novel immune checkpoint CEACAM1 to enhance anti-tumor immune activity. J Clin Oncol. 2016;34(15_suppl):3044.

[2]. Ben-Moshe T, et al. Humanized antibodies against CEACAM1. US20170044270A1. United States Patent and Trademark Office. 2017-02-16.

[3]. Reuveni H, et al. Anti carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) antibodies for inhibition of neutrophil extracellular traps (NET)-mediated activities. WO2024100663A1. World Intellectual Property Organization. 2024-05-16.

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

MOQ

Minimum order quantity

100 mg

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Activité biologique
- Description
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Essai clinique
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Application
- Biological Activity
Chemical Information
Pureté et documentation
Références

1/16

Immobilized CEACAM1 Protein, Human can bind CM-24. The EC₅₀ for this effect is 3.38 ng/mL.
1/16

Flow cytometric analysis of 1.5X10⁶ HT-29 cells with CM-24 (HY-P990569, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
1/16

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CM-24