NQO1 substrate
Based on 1 Customer Validation
NQO1 substrate acts as an efficient NQO1 substrate and may be a new option for the treatment of NQO1-overexpresssing drug-resistant NSCLC.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 98.02%
- CAS No.: 2304503-05-5
- Formule: C13H2F2N4O
- Masse moléculaire:268.18
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
1 μM
Compound: 20k
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Cytotoxicity against human A549 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human A549 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30508483] |
| A549/TR | IC50 |
0.6 μM
Compound: 20k
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Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30508483] |
| A549/TR | IC50 |
25.6 μM
Compound: 20k
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Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability in presence of DIC and measured after 72 hrs by MTT assay
Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability in presence of DIC and measured after 72 hrs by MTT assay
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[PMID: 30508483] |
| A549/TR | IC50 |
33.2 μM
Compound: 20k
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Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability in presence of NAC and measured after 72 hrs by MTT assay
Cytotoxicity against human A549/TR cells expressing NQO1 assessed as reduction in cell viability in presence of NAC and measured after 72 hrs by MTT assay
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[PMID: 30508483] |
| L02 | IC50 |
30.2 μM
Compound: 20k
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Cytotoxicity against NQO1 deficient human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against NQO1 deficient human L02 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30508483] |
| MCF7 | IC50 |
1.3 μM
Compound: 20k
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Cytotoxicity against human MCF7 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30508483] |
| MIA PaCa-2 | IC50 |
4.5 μM
Compound: 20k
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Cytotoxicity against human MIAPaCa2 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human MIAPaCa2 cells expressing NQO1 assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30508483] |
| NCI-H596 | IC50 |
19.1 μM
Compound: 20k
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Cytotoxicity against NQO1 deficient human NCI-H596 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against NQO1 deficient human NCI-H596 cells assessed as reduction in cell viability after 72 hrs by MTT assay
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[PMID: 30508483] |
Chemical Information
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CAS No. 2304503-05-5
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Appearance Solid
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Masse moléculaire 268.18
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Formule C13H2F2N4O
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Color Light yellow to yellow
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SMILES
N#CC1=C(C#N)N=C2C(C(C3=C2C=C(F)C(F)=C3)=O)=N1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 125 mg/mL (466.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (23.31 mM); Clear solution
This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7288 mL | 18.6442 mL | 37.2884 mL | 93.2210 mL |
| 5 mM | 0.7458 mL | 3.7288 mL | 7.4577 mL | 18.6442 mL | |
| 10 mM | 0.3729 mL | 1.8644 mL | 3.7288 mL | 9.3221 mL | |
| 15 mM | 0.2486 mL | 1.2429 mL | 2.4859 mL | 6.2147 mL | |
| 20 mM | 0.1864 mL | 0.9322 mL | 1.8644 mL | 4.6610 mL | |
| 25 mM | 0.1492 mL | 0.7458 mL | 1.4915 mL | 3.7288 mL | |
| 30 mM | 0.1243 mL | 0.6215 mL | 1.2429 mL | 3.1074 mL | |
| 40 mM | 0.0932 mL | 0.4661 mL | 0.9322 mL | 2.3305 mL | |
| 50 mM | 0.0746 mL | 0.3729 mL | 0.7458 mL | 1.8644 mL | |
| 60 mM | 0.0621 mL | 0.3107 mL | 0.6215 mL | 1.5537 mL | |
| 80 mM | 0.0466 mL | 0.2331 mL | 0.4661 mL | 1.1653 mL | |
| 100 mM | 0.0373 mL | 0.1864 mL | 0.3729 mL | 0.9322 mL |
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