ChlaDUB1-IN-2
ChlaDUB1-IN-2 (Compound 27a) is the inhibitor for Chlamydia trachomatis deubiquitinase ChlaDUB1 with an IC50 of 0.97 μM. ChlaDUB1-IN-2 inhibits the inclusion body formation of C. trachomatis with an IIC50 of 25.6 μg/mL.
For research use only. We do not sell to patients.
- Formula: C22H16N6O2
- Molecular Weight:396.40
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
IC50: ChlaDUB1 (0.97 μM)
Chemical Information
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Molecular Weight 396.40
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Formula C22H16N6O2
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SMILES
O=C(N1N=C(C2=C1C=CC=C2)CNC(C3=NC(C#N)=NC=C3)=O)CC4=CC=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Inclusion-body expression, solubilization, refolding and purification
Inclusion-body recovery uses insoluble recombinant protein aggregates from E. coli as a starting material; the workflow is cell disruption, inclusion-body isolation/washing, denaturant or mild solubilization, refolding into soluble protein, and final chromatographic purification. The readouts are soluble protein recovery, purity by SDS-PAGE/chromatography, structural recovery by methods such as circular dichroism when used, and biological activity when an assay is available.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ChlaDUB1-IN-2 Related Classifications
HY-172220 Related Classifications
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