Cholera autoinducer 1
Based on 1 Customer Validation
Cholera autoinducer 1 (CAI-1) is the major autoinducer in quorum sensing of V. cholerae. Cholera autoinducer 1 accumulates extracellularly as cell density increases, can be detected by V. cholerae, and signals through CqsS to regulate gene expression and inhibit biofilm formation and virulence factor production. Cholera autoinducer 1 can be used for cholera research.
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- Purity : 99.32%
- CAS No.: 1004296-82-5
- 화학식: C13H26O2
- 분자량:214.34
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보관:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
Cholera autoinducer 1 is released into the culture medium by CqsA-overexpressing E. coli at least 100-fold more than amino-CAI-1; synthetic CAI-1 is less stable than amino-CAI-1 in LB medium and potassium phosphate buffer[2].
Cholera autoinducer 1 is interconverted by E. coli MC4100 ara+ imp4213, with approximately 6% of exogenous CAI-1 converted to amino-CAI-1 and approximately 23% of amino-CAI-1 converted to CAI-1, whereas the interconversion in V. cholerae cannot be confirmed due to technical reasons[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1004296-82-5
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Appearance Oil
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분자량 214.34
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화학식 C13H26O2
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Color Colorless to light yellow
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SMILES
CCCCCCCCCC([C@@H](O)CC)=O
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Synonyms
CAI-1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
In Vitro:
DMSO : 50 mg/mL (233.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (5.83 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (5.83 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.6655 mL | 23.3274 mL | 46.6548 mL | 116.6371 mL |
| 5 mM | 0.9331 mL | 4.6655 mL | 9.3310 mL | 23.3274 mL | |
| 10 mM | 0.4665 mL | 2.3327 mL | 4.6655 mL | 11.6637 mL | |
| 15 mM | 0.3110 mL | 1.5552 mL | 3.1103 mL | 7.7758 mL | |
| 20 mM | 0.2333 mL | 1.1664 mL | 2.3327 mL | 5.8319 mL | |
| 25 mM | 0.1866 mL | 0.9331 mL | 1.8662 mL | 4.6655 mL | |
| 30 mM | 0.1555 mL | 0.7776 mL | 1.5552 mL | 3.8879 mL | |
| 40 mM | 0.1166 mL | 0.5832 mL | 1.1664 mL | 2.9159 mL | |
| 50 mM | 0.0933 mL | 0.4665 mL | 0.9331 mL | 2.3327 mL | |
| 60 mM | 0.0778 mL | 0.3888 mL | 0.7776 mL | 1.9440 mL | |
| 80 mM | 0.0583 mL | 0.2916 mL | 0.5832 mL | 1.4580 mL | |
| 100 mM | 0.0467 mL | 0.2333 mL | 0.4665 mL | 1.1664 mL |
Cholera autoinducer 1 Related Classifications
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