Cilinaphthalide A
Cilinaphthalide A is an arylnaphthalene lignan lactone that exhibits cytotoxic activity against bladder cancer and cervical cancer cell lines and possesses antibacterial activity against Escherichia coli. Cilinaphthalide A can be used for research on bladder cancer and cervical cancer.
For research use only. We do not sell to patients.
- CAS No.: 238423-03-5
- Formula: C22H20O7
- Molecular Weight:396.40
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| T-24 | ED50 |
1.3 μg/mL
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Cytotoxicity against human T-24 cervical carcinoma cells.
Cytotoxicity against human T-24 cervical carcinoma cells.
|
42152709 |
| Ca-Ski | ED50 |
3 μg/mL
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Cytotoxicity against human CaSki cervical carcinoma cells.
Cytotoxicity against human CaSki cervical carcinoma cells.
|
42152709 |
| HT-3 | ED50 |
0.3 μg/mL
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Cytotoxicity against human HT-3 cervical carcinoma cells.
Cytotoxicity against human HT-3 cervical carcinoma cells.
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42152709 |
| HT-3 | ED50 |
2.6 μg/mL
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Cytotoxicity against human HT-3 cervical carcinoma cells.
Cytotoxicity against human HT-3 cervical carcinoma cells.
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42152709 |
| SiHa | ED50 |
6.4 μg/mL
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Cytotoxicity against human cervical carcinoma SiHa cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
Cytotoxicity against human cervical carcinoma SiHa cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
|
10425143 |
| PLC-PRF-5 | ED50 |
12.0 μg/mL
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Cytotoxicity against human hepatoma PLC/PRF/5 cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
Cytotoxicity against human hepatoma PLC/PRF/5 cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
|
10425143 |
| NIH3T3 | ED50 |
> 8.0 μg/mL
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Cytotoxicity against inducible Ha-ras oncogene transformed NIH/3T3 212 cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
Cytotoxicity against inducible Ha-ras oncogene transformed NIH/3T3 212 cells assessed as reduction in cell viability incubated for 6 days by MTT assay.
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10425143 |
In Vitro
Cilinaphthalide A (compound 28) exhibits cytotoxic activity against the human cervical cancer cell lines T-24, CaSki, and HT-3, with ED50 values of 1.3, 3, and 0.3 μg/mL, respectively[1].
Cilinaphthalide A (6 days) exhibits low cytotoxicity against 212 cells, with an ED50 greater than 8.0 µg/mL[3].
Cilinaphthalide A (compound 4) exhibits antibacterial activity against Escherichia coli CMCC44103, Staphylococcus aureus CMCC26001, Salmonella typhimurium CMCC80087, Shigella flexneri CMCC51335, and Salmonella paratyphi B, with the lowest MIC of 12.5 μg/mL against Escherichia coli CMCC44103[2].
Cilinaphthalide A (compound 1) (6 days) exhibits significant in vitro cytotoxicity against T-24, CaSki, SiHa, HT-3, and PLC/PRF/5 cell lines, with the strongest activity against HT-3 cells (ED50 = 0.3 µg/mL)[3].
Cilinaphthalide A (5 μg/mL) shows no cytotoxicity against HCT-8 and Bel-7402 cells at concentrations up to 5 μg/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 238423-03-5
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Molecular Weight 396.40
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Formula C22H20O7
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SMILES
O=C1OCC=2C(OC)=C3C=C(OC)C(OC)=CC3=C(C=4C=CC(O)=C(OC)C4)C12
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Bacterial live/dead nucleic-acid viability staining
The LIVE/DEAD bacterial viability staining method is based on differential permeability of nucleic-acid-binding fluorescent dyes, most commonly SYTO 9 and propidium iodide (PI), which enables discrimination of bacterial populations with intact versus compromised cytoplasmic membranes. SYTO 9 penetrates both intact and damaged bacterial membranes and binds nucleic acids to produce green fluorescence, whereas propidium iodide penetrates only cells with compromised membranes and fluoresces red while also reducing SYTO 9 signal through competitive binding and fluorescence interactions. The resulting fluorescence pattern is interpreted as a proxy for membrane integrity, which is widely used as an indicator of bacterial viability in microscopy, flow cytometry, and spectroscopic platforms. However, mechanistic studies show that SYTO 9 and PI interactions involve displacement and fluorescence resonance energy transfer effects, which can influence signal interpretation depending on dye ratios a
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Cilinaphthalide A Related Classifications
HY-N21712 Related Classifications
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