CMLD010509
Based on 1 Customer Validation
CMLD010509 (SDS-1-021) is a highly specific inhibitor of the oncogenic translation program supporting multiple myeloma (MM)-including key oncoproteins such as MYC, MDM2, CCND1, MAF, and MCL-1. CMLD010509 (SDS-1-021) shows an IC50 below 10 nM for most MM cell lines and induces apoptosis. CMLD010509 (SDS-1-021) is a potent and selective translation inhibitor through an eIF4E phosphorylation-independent mechanism.
For research use only. We do not sell to patients.
- Purity: 99.11%
- CAS No.: 256497-58-2
- Formula: C27H26BrNO7
- Molecular Weight:556.40
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
CMLD010509 exerts potent inhibitory activity against most multiple myeloma (MM) cell lines with IC50 values less than 10 nM[1].
CMLD010509 (100 nM, 2 h) disrupts the oncogenic translation program in NCI-H929 cells[1].
CMLD010509 (0-1000 nM, 3-24 h) strongly induces an apoptotic response in NCl-H929 and MM1S cells, as measured by caspase-3 and caspase-7 activation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:MMIS xenograft SCID mouse model[1]
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Dosage:0.7 mg/kg
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Administration:Intraperitoneal injection (i.p.) twice a week
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Result:Revealed a robust decrease of M-spike in mice at week 4, which was associated with a significantly prolonged survival.
Chemical Information
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CAS No. 256497-58-2
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Appearance Solid
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Molecular Weight 556.40
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Formula C27H26BrNO7
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Color White to off-white
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SMILES
O=C([C@H]([C@H]1C2=CC=CC=C2)[C@@H](O)[C@]3(O)[C@@]1(C4=CC=C(Br)C=C4)OC5=CC(OC)=CC(OC)=C35)NOC
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Synonyms
SDS-1-021
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 60 mg/mL (107.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (5.39 mM); Clear solution
This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7973 mL | 8.9863 mL | 17.9727 mL | 44.9317 mL |
| 5 mM | 0.3595 mL | 1.7973 mL | 3.5945 mL | 8.9863 mL | |
| 10 mM | 0.1797 mL | 0.8986 mL | 1.7973 mL | 4.4932 mL | |
| 15 mM | 0.1198 mL | 0.5991 mL | 1.1982 mL | 2.9954 mL | |
| 20 mM | 0.0899 mL | 0.4493 mL | 0.8986 mL | 2.2466 mL | |
| 25 mM | 0.0719 mL | 0.3595 mL | 0.7189 mL | 1.7973 mL | |
| 30 mM | 0.0599 mL | 0.2995 mL | 0.5991 mL | 1.4977 mL | |
| 40 mM | 0.0449 mL | 0.2247 mL | 0.4493 mL | 1.1233 mL | |
| 50 mM | 0.0359 mL | 0.1797 mL | 0.3595 mL | 0.8986 mL | |
| 60 mM | 0.0300 mL | 0.1498 mL | 0.2995 mL | 0.7489 mL | |
| 80 mM | 0.0225 mL | 0.1123 mL | 0.2247 mL | 0.5616 mL | |
| 100 mM | 0.0180 mL | 0.0899 mL | 0.1797 mL | 0.4493 mL |
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