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Cat. No.: HY-P2893

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Creatinase, Actinobacteria

(Synonyms: Creatine amidohydrolase)

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Creatinase, Actinobacteria (Creatine amidohydrolase) is a hydrolase that catalyzes the hydrolysis of creatine into sarcosine and urea, and plays an important role in the enzymatic determination of creatinine concentration.

For research use only. We do not sell to patients.

CAS No.: 37340-58-2

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Creatinase, Actinobacteria Chemical Structure

CAS No.: 37340-58-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Endogenous Metabolite Isoforms :

In Vitro

Product Information
This product can hydrolyze creatine and is used in the development and preparation of enzymatic creatinine reagents.
Molecular weight: 48 kDa (SDS-PAGE)
Isoelectric point: 4.6
Inhibitors: Ag ⁺, Cu ²⁺, Hg ²⁺
Optimum: pH 7.5
Optimum temperature: 45-50°C
pH stability 4.5-10.0 (25°C, 16 h)

Instructions
Can be dissolved in water (1 mg/mL), or according to specific experimental references (such as using 50 mM PBS, pH 7.5).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EC Number

3.5.3.3

Enzyme Activity

≥10 U/mg soild

Unit Definition

One unit is defined as the amount of enzyme that can react creatine to produce 1 μmol sarcosine per minute at 37°C and pH 5.5.

Chemical Information

CAS No. 37340-58-2
Appearance Solid
Color White to off-white
SMILES

[Creatinase, Actinobacteria]
Synonyms

Creatine amidohydrolase
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (Need ultrasonic)

View the Complete Stock Solution Preparation Table

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (264 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. J Schumann, et al. Stabilization of creatinase from Pseudomonas putida by random mutagenesis. Protein Sci. 1993 Oct;2(10):1612-20. [Content Brief]

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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Biological Activity
- Description
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References

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Creatinase, Actinobacteria

All Endogenous Metabolite Isoforms