CRT 0105446
CRT 0105446 (compound 22d) is a LIMK1 inhibitor, with an IC50 of 8 nM.
For research use only. We do not sell to patients.
- CAS No.: 1661846-05-4
- Formula: C20H18F3N3O2S
- Molecular Weight:421.44
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Sf21 | IC50 |
0.008 μM
Compound: 22d
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Inhibition of recombinant N-terminal 6His-tagged LIMK1 (unknown origin) expressed in Sf21 cells by HTRF assay using cofilin as a substrate
Inhibition of recombinant N-terminal 6His-tagged LIMK1 (unknown origin) expressed in Sf21 cells by HTRF assay using cofilin as a substrate
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[PMID: 26356364] |
| ZR-75-1 | IC50 |
2 μM
Compound: 22d
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Inhibition of LIMK1 in human ZR75-1 cells assessed as inhibition of cofilin phosphorylation after 5 hrs
Inhibition of LIMK1 in human ZR75-1 cells assessed as inhibition of cofilin phosphorylation after 5 hrs
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[PMID: 26356364] |
In Vitro
CRT 0105446 (compound 22d) inhibits the invasion of the cells through matrigel at a concentration of 3 µM, whilst not significantly affecting cell viability even at the higher compound concentration of 10 µM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1661846-05-4
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Molecular Weight 421.44
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Formula C20H18F3N3O2S
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SMILES
CC(C(NC1=NC=C(S1)C2=C(C=NC=C2)C3=CC=C(C=C3C(F)(F)F)OC)=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
CRT 0105446 Related Classifications
HY-123345 Related Classifications
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