IL-4 Protein, Human (HEK293)

IL-4 Protein, Human (HEK293) is a potent lymphoid cell growth factor, which stimulates the growth and survivability of B-cells and T-cells. IL-4 Protein, Human (HEK293) is a recombinant human interleukin-4 (rhIL-4) expressed in HEK293 cells.

Interleukin-4 (IL-4) is a cytokine that plays an important role in regulating inflammation and immune responses. It induces the differentiation of naïve helper T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13. IL-4, originally designated as B-cell growth factor-1 (BSFl), is described in the supernatant of activated EL-4 thymoma cells as a factor that can co-stimulate B-cells activated with submitogenic concentrations of anti-IgM^[1].

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is ≤0.247 ng/mL, corresponding to a specific activity is ≥4.049×10⁶ units/mg.

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  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.1732 ng/mL, corresponding to a specific activity is 5.774×10⁶ U/mg

Human

HEK293

Tag Free

P05112-1 (H25-S153)

3565  [NCBI]

Full Length of Isoform-1 Mature Protein

HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

Approximately 19-25 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/pc-3-cell.html  [Content Brief]

  [2]. Le HV, et al. Isolation and characterization of multiple variants of recombinant human interleukin 4 expressed in mammalian cells. J Biol Chem. 1988 Aug 5;263(22):10817-23.  [Content Brief]

  [3]. Melih Ö Celik, et al. IL-4 induces M2 macrophages to produce sustained analgesia via opioids. JCI Insight. 2020 Feb 27;5(4):e133093.  [Content Brief]