DAO-IN-2
Based on 1 publication(s) in Google Scholar
DAO-IN-2 is a d-amino acid oxidase (DAO) inhibitor (h-DAO IC50 = 245 nM). DAO-IN-2 is a moderate inhibitor of DAAO in vitro and in vivo. DAO-IN-2 can be used to study DAAO-related psychiatric disorders.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 39793-31-2
- Formula: C7H5NO2S
- Molecular Weight:167.19
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DAO-IN-2
MoreAll Amino Acid Oxidase Isoforms
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Biological Activity
DAO-IN-2 (Compound 8) has no off-target activities for the inhibition of P450 enzymes (IC50s > 10 μM for CYP3A4, CYP2D6 and CYP3C9) or binding to ion channels[1].
DAO-IN-2 inhibits D-amino acid oxidase (DAAO) activity against expressing either the human or rat DAAO gene CHO cells, with IC50s of 144.6, 113.9 nM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Wistar rats (adult male) model[2]
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Dosage:10, 25, 50, 100, 200 mg/kg
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Administration:i.p., once
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Result:Inhibited kidney DAAO activity both time- and dose-dependent, elevated plasma D-serine levels, did not alter circulating levels of glycine or D-alanine.
Inhibited DAAO activity in the cerebellum, increased CSF levels of D-serine.
Reduced spontaneous activity on amphetamine-induced psychomotor activity.
Chemical Information
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CAS No. 39793-31-2
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Appearance Solid
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Molecular Weight 167.19
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Formula C7H5NO2S
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SMILES
O=C(C1=CC2=C(C=CS2)N1)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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FEBS Open Bio
Establishing an assay to evaluate d-amino acid oxidase enzyme kinetics and inhibition using WST-8 redox dye. [Abstract]2026 Feb 24. PMID: 41732867
Solvent & Solubility
DMSO : 100 mg/mL (598.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (7.48 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (7.48 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Tim Sparey, et, al. The discovery of fused pyrrole carboxylic acids as novel, potent D-amino acid oxidase (DAO) inhibitors. Bioorg Med Chem Lett. 2008,18, 11. [Content Brief]
[2]. Smith SM, et al. The behavioral and neurochemical effects of a novel D-amino acid oxidase inhibitor compound 8 [4H-thieno [3,2-b]pyrrole-5-carboxylic acid] and D-serine. J Pharmacol Exp Ther. 2009 Mar;328(3):921-30. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.9812 mL | 29.9061 mL | 59.8122 mL | 149.5305 mL |
| 5 mM | 1.1962 mL | 5.9812 mL | 11.9624 mL | 29.9061 mL | |
| 10 mM | 0.5981 mL | 2.9906 mL | 5.9812 mL | 14.9530 mL | |
| 15 mM | 0.3987 mL | 1.9937 mL | 3.9875 mL | 9.9687 mL | |
| 20 mM | 0.2991 mL | 1.4953 mL | 2.9906 mL | 7.4765 mL | |
| 25 mM | 0.2392 mL | 1.1962 mL | 2.3925 mL | 5.9812 mL | |
| 30 mM | 0.1994 mL | 0.9969 mL | 1.9937 mL | 4.9843 mL | |
| 40 mM | 0.1495 mL | 0.7477 mL | 1.4953 mL | 3.7383 mL | |
| 50 mM | 0.1196 mL | 0.5981 mL | 1.1962 mL | 2.9906 mL | |
| 60 mM | 0.0997 mL | 0.4984 mL | 0.9969 mL | 2.4922 mL | |
| 80 mM | 0.0748 mL | 0.3738 mL | 0.7477 mL | 1.8691 mL | |
| 100 mM | 0.0598 mL | 0.2991 mL | 0.5981 mL | 1.4953 mL |
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