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  3. Fluorescent Dye
  4. DASPEI

DASPEI is a cationic styrenyl mitochondrial dye with large Stokes shift. DASPEI has excitation and emission wavelength at 550/573 nm, which has good light chromogenic property. DASPEI can stain mitochondria in living cells with good labeling property. And DASPEI can also be used to stain presynaptic nerve endings independently of neuronal activity.

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DASPEI Chemical Structure

DASPEI Chemical Structure

CAS No. : 3785-01-1

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DASPEI Related Antibodies

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Description

DASPEI is a cationic styrenyl mitochondrial dye with large Stokes shift. DASPEI has excitation and emission wavelength at 550/573 nm, which has good light chromogenic property. DASPEI can stain mitochondria in living cells with good labeling property. And DASPEI can also be used to stain presynaptic nerve endings independently of neuronal activity[1].

In Vitro

Preparation of DASPEI working solution
1) Preparation of the stock solution
Dissolve 1 mg of DASPEI in 0.2016 mL of DMSO to obtain 10 mM of DASPEI
Note: It is recommended to store the stock solution at -20 ℃ -80 ℃ away from light and avoid repetitive freeze-thaw cycles.
2) Preparation of DASPEI working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 0.5-1 mM of DASPEI working solution.
Note: Please adjust the concentration of DASPEI working solution according to the actual situation.


Cell staining
1) For suspension cells: Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2) Add 1 mL of DASPEI working solution, and then incubate at room temperature for 10-30 minutes.
3) Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
4) Wash twice with PBS, 5 minutes each time.
5) Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DASPEI Related Antibodies
Molecular Weight

380.27

Formula

C17H21IN2
CAS No.
Appearance

Solid

Color

Purple to purplish red

Emission (Em)

573
Excitation (Ex)

550
SMILES

CN(C)C(C=C1)=CC=C1/C=C/C2=[N+](CC)C=CC=C2.[I-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 41.67 mg/mL (109.58 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6297 mL 13.1486 mL 26.2971 mL
5 mM 0.5259 mL 2.6297 mL 5.2594 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.86%

COA (246 KB) HNMR (248 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6297 mL 13.1486 mL 26.2971 mL 65.7428 mL
5 mM 0.5259 mL 2.6297 mL 5.2594 mL 13.1486 mL
10 mM 0.2630 mL 1.3149 mL 2.6297 mL 6.5743 mL
15 mM 0.1753 mL 0.8766 mL 1.7531 mL 4.3829 mL
20 mM 0.1315 mL 0.6574 mL 1.3149 mL 3.2871 mL
25 mM 0.1052 mL 0.5259 mL 1.0519 mL 2.6297 mL
30 mM 0.0877 mL 0.4383 mL 0.8766 mL 2.1914 mL
40 mM 0.0657 mL 0.3287 mL 0.6574 mL 1.6436 mL
50 mM 0.0526 mL 0.2630 mL 0.5259 mL 1.3149 mL
60 mM 0.0438 mL 0.2191 mL 0.4383 mL 1.0957 mL
80 mM 0.0329 mL 0.1644 mL 0.3287 mL 0.8218 mL
100 mM 0.0263 mL 0.1315 mL 0.2630 mL 0.6574 mL
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DASPEI Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DASPEI3785-01-1Fluorescent DyeInhibitorinhibitorinhibit

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