FWG-33B
FWG-33B is an activator of TRMT112:METTL5 complex with an IC50 value of 0.47 μM. FWG-33B can be used for the research of breast carcinoma, hepatocellular carcinoma, nasopharyngeal carcinoma.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Formel: C24H25ClN2O3
- Molecular Weight:424.92
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
FWG-33B (5 μM; 4 hours) engages ~40% of total TRMT112 and >90% of METTL5-complexed TRMT112 in HCT116TRMT112-FLAG:HA-METTL5 cells[1].
FWG-33B (0.01-50 μM; 3 hours pretreatment) stereoselectively engages TRMT112 in HCT116TRMT112-FLAG:HA-METTL5 cells with an IC50 of 1.0 μM[1].
FWG-33B (40 μM; 30 minutes) enhances the methyltransferase activity of WT-TRMT112:METTL5 (but not TRMT112C100A:METTL5) by ~2-fold with 12-nt and 60-nt rRNA substrates[1].
FWG-33B (40 μM; 30 minutes) increases the catalytic efficiency of WT-TRMT112:METTL5 by reducing the Km for rRNA substrate from 10 μM to 4.7 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 424.92
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Formel C24H25ClN2O3
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SMILES
CC(NC(C=C1)=CC=C1O[C@H]2[C@H]3[C@H](CN(C(C=C)=O)C3)[C@@H](C4=CC(Cl)=CC=C4)C2)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
FWG-33B Related Classifications
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