Coelenterazine
Based on 6 publication(s) in Google Scholar
Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine has been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe and it can also be used in chemiluminescent detection of peroxynitrite (Ex/Em = 429/466 nm).
For research use only. We do not sell to patients.
- Purity: 98.34%
- CAS No.: 55779-48-1
- Formula: C26H21N3O3
- Molecular Weight:423.46
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Storage:
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Coelenterazine
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Biological Activity
HCT-8 control cells, transiently expressing Renilla luciferase (RLuc), showed low bioluminescence due to P-glycoprotein–mediated efflux transport of coelenterazine. By comparison, transiently expressing RLuc HCT-8 cells, wherein P-glycoprotein was down-regulated with shRNAi, showed high bioluminescence[3].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of free radical production[5].
1. The measurements are performed in 0.1 M potassium phosphate buffer, pH 7.0.
2. Coelenterazine (2.5 μM) is added to the buffer immediately prior
to measurements.
3. Add 2 mg/mL NADH.
4. At room temperature using a luminometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Coelenterazine (2 mg/kg) is injected intravenously.
After 5 minutes, the mice are fixed and imaged for 3 to 5 minutes.
All mice demonstrated very high Rluc activity at the primary site with the majority of mice simultaneously showing metastases to inguinal ILNs[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 55779-48-1
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Appearance Solid
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Molecular Weight 423.46
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Formula C26H21N3O3
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Color Light yellow to yellow
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SMILES
O=C1C(CC2=CC=C(O)C=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=C(O)C=C5)=CN31
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Structure Classification
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Initial Source
marine bioluminescent organisms
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (6)
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Journal Impact Factor
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Most Recent
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ACS Sens
Secretory Bioluminescent Reporter Sensor for Functional Assessment of Glucocorticoids In Vitro and In Vivo. [Abstract]2026 Jun 26. PMID: 42358115 -
Plant Cell Environ
A Rapid and Reversible Molecular "Switch" Regulating Protein Expression in Chlamydomonas reinhardtii. [Abstract]2025 Jun;48(6):3913-3924. PMID: 39838873 -
Prostate Cancer Prostatic Dis
Effects of dietary omega-3 fatty acids on orthotopic prostate cancer progression, tumor associated macrophages, angiogenesis and T-cell activation-dependence on GPR120. [Abstract]2022 Sep;25(3):539-546. PMID: 35075215 -
FASEB J
2025 Jun 15;39(11):e70624. PMID: 40444459 -
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Solvent & Solubility
Ethanol : 1 mg/mL (2.36 mM; ultrasonic and warming and heat to 60°C; DMSO can inactivate Coelenterazine's activity)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Cells are plated at a density of 5×104 cells per well into 24-well plates and grown to 80-100% confluency. Just before imaging, media are changed to a colorless solution containing (in mM): 2.7 KCl, 139 NaCl, 8.1 Na2HPO4, 0.7 H2O, 1.5 KH2PO4, 1.8 CaCl2, 1 MgCl2 and 5.5 d-glucose. Cells are preincubated for 15 min in the absence or presence of Pgp modulator, after which Coelenterazine (final concentration of 470 nM) is added directly to the cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice are anesthetized with metofane before tail vein injection of Coelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). Bioluminescence imaging is performed on the in vivo imaging system at 2, 6, 8, and 11 min after injection. After imaging, animals are killed by cervical dislocation; tumors are then harvested and weighed[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Markova SV, et al, Coelenterazine-dependent luciferases. Biochemistry (Mosc). 2015 Jun;80(6):714-32. [Content Brief]
[2]. Lucas M, et al. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: its usefulness in the assay of respiratory burst in neutrophils. Anal Biochem. 1992 Nov 1;206(2):273-7. [Content Brief]
[3]. Pichler A, et al. In vivo RNA interference-mediated ablation of MDR1 P-glycoprotein. Clin Cancer Res. 2005 Jun 15;11(12):4487-94. [Content Brief]
[4]. Volk-Draper LD, et al. Novel model for basaloid triple-negative breast cancer: behavior in vivo and response to therapy. Neoplasia. 2012 Oct;14(10):926-42. [Content Brief]
[5]. Raha S, et al. Control of oxygen free radical formation from mitochondrial complex I: roles for protein kinase A and pyruvate dehydrogenase kinase. Free Radic Biol Med. 2002 Mar 1;32(5):421-30. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 2.3615 mL | 11.8075 mL | 23.6150 mL | 59.0375 mL |
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