Cat. No.: HY-N9921

Antcin A

Name: Antcin A
Brand: MedChemExpress
SKU: HY-N9921
Price: 280.00 USD
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Antcin A is an orally active modulator of p53 and glucocorticoid receptor (GR), with an IC₅₀ of 8.55 μM against human GR. Through transcriptional activation of p53, Antcin A induces miR-200c and inhibits ZEB1, regulates epithelial-mesenchymal transition markers, and suppresses cancer cell migration/invasion. Antcin A activates GR, inhibits Na⁺/K⁺-ATPase and NLRP3 inflammasome assembly, pyroptosis (pyroptosis) and pro-inflammatory cytokine release, reduces hepatic lipid deposition, improves liver function, and reverses metabolic changes induced by the SARS-CoV-2 spike protein. Antcin A can be used in research related to breast cancer, head and neck cancer, non-alcoholic fatty liver disease, and coronavirus disease 2019 (COVID-19).

For research use only. We do not sell to patients.

Purity : 99.71%
CAS No.: 163597-24-8
Formula: C₂₉H₄₂O₄
Molecular Weight:454.64

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Antcin A Chemical Structure

CAS No.: 163597-24-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

^[4]

NLRP3

All NOD-like Receptor (NLR) Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
KB	IC₅₀	179.6 μM	Cytotoxicity against human head and neck cancer KB cells, quantified as reduction of 50% cell survival. Cytotoxicity against human head and neck cancer KB cells, quantified as reduction of 50% cell survival.	31883608
Neutrophil	IC₅₀	8.55 μM	Inhibition of fMLP-induced superoxide generation in cultured human peripheral neutrophils. Inhibition of fMLP-induced superoxide generation in cultured human peripheral neutrophils.	31883608

In Vitro

Anti-cancerInnate ImmunityOxidative StressPyroptosisInflammasomeCytokines & ChemokinesApoptosisOthers

Anti-cancer

Antcin A (87.6-188.1 μM) selectively reduces cell survival in human TSCCa, GNM, and KB head and neck cancer cell lines with IC₅₀ of 188.1 μM, 87.6 μM, and 179.6 μM, respectively^[2].
Antcin A (0.1-10 μM; 1 h) induces glucocorticoid receptor nuclear translocation in human lung cancer A549 cells, with a minimal effective concentration of 10 μM for 1 h incubation^[3].

Innate Immunity

Oxidative Stress

Antcin A inhibits fMLP-induced superoxide generation in human peripheral neutrophils with an IC₅₀ of 8.55 μM, exhibiting greater potency than ibuprofen^[2].

Pyroptosis

Inflammasome

Cytokines & Chemokines

Antcin A (5-15 mg/L; 6 h pre-incubation, 12 h LPS/Nigericin treatment) dose-dependently inhibits pyroptosis and NLRP3 inflammasome activation in mouse liver Kupffer cells, reducing inflammatory factor secretion, membrane permeability, and cleavage of GSDMD-FL^[4].

Apoptosis

Antcin A (15 mg/L; 6-48 h) is non-toxic to mouse liver Kupffer cells, with no effect on cell viability or apoptosis over a 48-hour incubation period^[4].

Others

Antcin A induces glucocorticoid receptor activation in human lung A549 cells, exerting an anti-inflammatory effect comparable to synthetic glucocorticoids via high-affinity binding to GR^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[4]

Cell Line:

Kupffer cells
Concentration:

15 mg/L
Incubation Time:

6, 12, 24, 48 h
Result:

Showed no effect on cell apoptosis over a 48-hour incubation period.

In Vivo

Lipid MetabolismCytokines & ChemokinesInflammasomePyroptosisOthers

Lipid Metabolism

Cytokines & Chemokines

Inflammasome

Pyroptosis

Antcin A (5-10 μg/mL; p.o.; once daily for 28 days) dose-dependently alleviates hepatic lipid accumulation, inflammation, and liver damage in high-fat diet-induced NAFLD mice by inhibiting NLRP3 inflammasome activation and pyroptosis^[4].

Others

Antcin A (10 mg/kg) does not cause observable physiological changes or mortality in mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J wild-type mice^[4]
Dosage:

5, 10 μg/mL
Administration:

p.o.; once daily for 28 days
Result:

Reduced hepatic lipid accumulation in a dose-dependent manner, with the high-dose group showing a more pronounced effect.
Attenuated liver tissue inflammation, reduced bubble-like structural changes, and lowered serum levels of ALT and AST.
Decreased the levels of inflammatory factors IL-1β, IL-18, and TNF-α in both peripheral blood and liver tissue.
Inhibited activation of the NLRP3 inflammasome, downregulated protein expression of NLRP3 and Caspase-1, and suppressed cleavage of GSDMD-FL to GSDMD-NT in liver tissue, with the high-dose group showing greater inhibition.

Related Protocols :

Chemical Information

CAS No. 163597-24-8
Appearance Solid
Molecular Weight 454.64
Formula C₂₉H₄₂O₄
Color Off-white to light yellow
SMILES

C[C@@]12C3=C(CC[C@@]1([H])[C@@H](C(CC2)=O)C)[C@@]4([H])[C@](CC3=O)([C@@](CC4)([H])[C@H](C)CCC(C(C)C(O)=O)=C)C
Structure Classification
- Steroids
Initial Source
- Microorganism
Antrodia cinnamomea
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Senthil Kumar K J, et al. Antcin‑A Modulates Epithelial‑to‑Mesenchymal Transition and Inhibits Migratory and Invasive Potentials of Human Breast Cancer Cells via p53‑Mediated miR‑200c Activation. Planta Med. 2019, 85, 09/10.

[2]. Senthil Kumar KJ, et al. A mechanistic and empirical review of antcins, a new class of phytosterols of formosan fungi origin. Journal of food and drug analysis. 2020 Jan;28(1):38-59.

[3]. Chen YC, et al. Antcin A, a steroid-like compound from Antrodia camphorata, exerts anti-inflammatory effect via mimicking glucocorticoids. Acta pharmacologica Sinica. 2011 Jul;32(7):904-11.

[4]. Ruan S, et al. Antcin A alleviates pyroptosis and inflammatory response in Kupffercells of non-alcoholic fatty liver disease by targeting NLRP3. Int Immunopharmacol. 2021 Nov;100:108126. [Content Brief]

[5]. Dakpa G, et al. Antcin A, a phytosterol regulates SARS-CoV-2 spike protein-mediated metabolic alteration in THP-1 cells explored by the H-NMR-based metabolomics approach. Phytotherapy research : PTR. 2023 Mar;37(3):885-902.

[6]. I-Hwa Cherng, et al. Three New Triterpenoids from Antrodia cinnamomea. J. Nat. Prod. 1 March 1995; 58 (3): 365–371.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Antcin A

All NOD-like Receptor (NLR) Isoforms