Pozelimab
Based on 1 Customer Validation
Pozelimab (REGN3918) is a fully human IgG4 anti-C5 monoclonal antibody. Pozelimab binds to C5 and C5 variants with high affinity and blocks complement-mediated hemolysis. Pozelimab can be used for the research of complement-mediated diseases.
For research use only. We do not sell to patients.
- Purity: 99.42%
- CAS No.: 2096328-94-6
- Molecular Weight:144.78 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Human IgG4 kappa
Human
Complement C5
Pozelimab binds with human C5 and cynomolgus monkey C5 with Kd values of 262 pM and 8.55 nM, respectively at 37˚C, pH7.4[1]. Pozelimab blocks classic pathway (CP)-mediated hemolysis in normal human and cynomolgus monkey serum with IC50 values of 2.8 and 20 nM, respectively at 37˚C, pH7.4[1]. Pozelimab blocks alternative pathway (AP)-mediated hemolysis in normal human and cynomolgus monkey serum with IC50 values of 26 and 8.9 nM, respectively at 37˚C, pH7.4[1]. Pozelimab binds to human C5, cynomolgus monkey C5, human R885C C5 and human C5b,6 with Kd values of 0.189, 2.73, 0.422, 0.465 and 0.137 nM respectively at 25˚C, pH7.4[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Cynomolgus monkey[1]
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Dosage:15 mg/kg
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Administration:Intravenous injection; 15 mg/kg, once
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Result:Showed a a greater exposure to monkeys compared with H4H12161P and H4H12170P.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG4 kappa
ELISA, FACS, Functional assay
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Flow cytometric analysis of 1X106 HepG2 cells with Pozelimab (HY-P99786, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa Isotype Control (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS No. 2096328-94-6
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Appearance Liquid
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Molecular Weight 144.78 kDa
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Color Colorless to light yellow
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SMILES
[Pozelimab]
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Synonyms
REGN3918
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (259 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)