Dimethylamiloride
Based on 3 publication(s) in Google Scholar
Dimethylamiloride is a specific antiporter inhibitor.
For research use only. We do not sell to patients.
- Purity: 98.18%
- CAS No.: 1214-79-5
- Formula: C8H12ClN7O
- Molecular Weight:257.68
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Membrane Transporter/Ion Channel Compound Library
HY-L011
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Human Metabolite Library
HY-L123
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Membrane Protein-targeted Compound Library
HY-L149
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Membrane Receptor-targeted Compound Library
HY-L150
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Highly Selective Inhibitors Library
HY-L158
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Mass Spectrometry Human Metabolite Library
HY-L215
Publications Citing Use of MedChemExpress (MCE) Dimethylamiloride
More
Biological Activity
Antiporter[1]
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Cell Line
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Type | Value | Description | References |
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| HRPE | IC50 |
0.2 mM
Compound: 5-(N, N-dimethyl)amiloride
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TP_TRANSPORTER: inhibition of TEA uptake in Octn1-HRPE cells
TP_TRANSPORTER: inhibition of TEA uptake in Octn1-HRPE cells
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[PMID: 10825452] |
Chemical Information
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CAS No. 1214-79-5
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Appearance Solid
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Molecular Weight 257.68
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Formula C8H12ClN7O
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Color Light yellow to yellow
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SMILES
O=C(C1=NC(Cl)=C(N(C)C)N=C1N)NC(N)=N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Cell Metab
Liver-breast communication of adipocyte-oriented exosomes drives primary mammary cancer progression. [Abstract]2025 Sep 24:S1550-4131(25)00385-7. PMID: 40997802 -
J Dermatolog Treat
Engineering extracellular vesicles with macrophage membrane fusion for ameliorating imiquimod-induced psoriatic skin inflammation. [Abstract]2023 Dec;34(1):2220445. PMID: 38073229 -
Electrophoresis
Towards an Analytical Procedure Control Strategy for the Capillary Zone Electrophoresis Method for Monoclonal Antibodies: Alternatives for ε-Aminocaproic Acid and Triethylenetetramine. [Abstract]2025 Sep 23. PMID: 40988426
Solvent & Solubility
DMSO : 100 mg/mL (388.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.70 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.70 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8808 mL | 19.4039 mL | 38.8078 mL | 97.0196 mL |
| 5 mM | 0.7762 mL | 3.8808 mL | 7.7616 mL | 19.4039 mL | |
| 10 mM | 0.3881 mL | 1.9404 mL | 3.8808 mL | 9.7020 mL | |
| 15 mM | 0.2587 mL | 1.2936 mL | 2.5872 mL | 6.4680 mL | |
| 20 mM | 0.1940 mL | 0.9702 mL | 1.9404 mL | 4.8510 mL | |
| 25 mM | 0.1552 mL | 0.7762 mL | 1.5523 mL | 3.8808 mL | |
| 30 mM | 0.1294 mL | 0.6468 mL | 1.2936 mL | 3.2340 mL | |
| 40 mM | 0.0970 mL | 0.4851 mL | 0.9702 mL | 2.4255 mL | |
| 50 mM | 0.0776 mL | 0.3881 mL | 0.7762 mL | 1.9404 mL | |
| 60 mM | 0.0647 mL | 0.3234 mL | 0.6468 mL | 1.6170 mL | |
| 80 mM | 0.0485 mL | 0.2425 mL | 0.4851 mL | 1.2127 mL | |
| 100 mM | 0.0388 mL | 0.1940 mL | 0.3881 mL | 0.9702 mL |
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