Direct Blue 1 (Synonyms: Chicago Sky Blue 6B)

Cat. No.: HY-D0939 Purity: ≥95.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Direct Blue 1 (Chicago Sky Blue 6B) is a complex dye for background autofluorescence in immunofluorescence histochemistry. Direct Blue 1 is a potent and competitive VGLUT inhibitor. Direct Blue 1 can inhibit the Aβ-binding small molecule PrP ligand. Direct Blue 1 has anti-inflammatory activity.

For research use only. We do not sell to patients.

Direct Blue 1 Chemical Structure

CAS No. : 2610-05-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Description

In Vitro

Direct Blue 1 (0.1, 1, 10 μM, 9 days) can bind α-synuclein and inhibit its aggregation, thus blocking the proliferation of α-synuclein between SH-SY5Y cells^[4].
Direct Blue 1 (5, 10, 20 μM, 48 h) inhibits RANKL-induced osteoclasts and bone resorption in vitro by inhibiting NF-κB signaling and promoting proteasome-mediated MIF degradation^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[5]

Cell Line:	BMMs
Concentration:	0.75, 1.5, 3.125, 6.25, 12.5, 25, 50, 100, 200 μM
Incubation Time:	48, 96 h
Result:	Exhibited no cytotoxic effect at concentrations of 0-50 μM.

Western Blot Analysis^[5]

Cell Line:	BMMs
Concentration:	5, 10, 20 μM
Incubation Time:	0, 1, 3, 5 days
Result:	Attenuated the protein expression of both c-Fos and NFATc1. Decreased the protein expression of MIF.

In Vivo

Direct Blue 1 (2 or 8 mg/kg, subcutaneously, for 4 weeks) alleviates inflammatory bone destruction and osteoporosis induced by titanium particles in mice^[5].
Direct Blue 1 (7.5 μg, intraventricular injection) alleviates methamphetamine-induced hyperactivity and behavioral sensitization in mice^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	OVX-induced osteoporosis mice model^[5]
Dosage:	2, 8 m/kg
Administration:	s.c.
Result:	Inhibited the elevated osteoclast formation and bone resorption.

Animal Model:	METH-induced hyperactivity in mice^[6]
Dosage:	7.5 μg
Administration:	i.c.v.
Result:	Reduced acute hyperactivity and attenuated expression of sensitization.

Molecular Weight

992.80

Formula

C₃₄H₂₄N₆Na₄O₁₆S₄

CAS No.

2610-05-1

Appearance

Solid

Color

Light blue to blue

SMILES

COC1=CC(C2=CC=C(/N=N/C3=C(O)C4=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C4C=C3)C(OC)=C2)=CC=C1/N=N/C5=C(O)C6=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C6C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (125.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 2.08 mg/mL (2.10 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0073 mL	5.0363 mL	10.0725 mL
5 mM	0.2015 mL	1.0073 mL	2.0145 mL
10 mM	0.1007 mL	0.5036 mL	1.0073 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4.17 mg/mL (4.20 mM); Clear solution

This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 4.17 mg/mL (4.20 mM); Clear solution

This protocol yields a clear solution of ≥ 4.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (41.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 5 mg/mL (5.04 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥95.0%

Select Batch:

Data Sheet (280 KB) SDS (418 KB)

COA (251 KB) HNMR (327 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.0073 mL	5.0363 mL	10.0725 mL	25.1813 mL
DMSO	5 mM	0.2015 mL	1.0073 mL	2.0145 mL	5.0363 mL
	10 mM	0.1007 mL	0.5036 mL	1.0073 mL	2.5181 mL
	15 mM	0.0672 mL	0.3358 mL	0.6715 mL	1.6788 mL
	20 mM	0.0504 mL	0.2518 mL	0.5036 mL	1.2591 mL
	25 mM	0.0403 mL	0.2015 mL	0.4029 mL	1.0073 mL
	30 mM	0.0336 mL	0.1679 mL	0.3358 mL	0.8394 mL
	40 mM	0.0252 mL	0.1259 mL	0.2518 mL	0.6295 mL
	50 mM	0.0201 mL	0.1007 mL	0.2015 mL	0.5036 mL
	60 mM	0.0168 mL	0.0839 mL	0.1679 mL	0.4197 mL
	80 mM	0.0126 mL	0.0630 mL	0.1259 mL	0.3148 mL
	100 mM	0.0101 mL	0.0504 mL	0.1007 mL	0.2518 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Direct Blue 12610-05-1Chicago Sky Blue 6BDirect Blue1Direct Blue-1Inhibitorinhibitorinhibit

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