TMRE
Based on 77 publication(s) in Google Scholar
Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms.
For research use only. We do not sell to patients.
- Purity: 98.49%
- CAS No.: 115532-52-0
- Formula: C26H27ClN2O7
- Molecular Weight:514.95
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)
Publications Citing Use of MedChemExpress (MCE) TMRE
More- Sci Bull. 2025 May 16:S2095-9273(25)00531-6. [Abstract]
- Nat Aging. 2024 May 9. [Abstract]
- Autophagy. 2023 Aug;19(8):2240-2256. [Abstract]
- Autophagy. 2021 Dec;17(12):4341-4362. [Abstract]
- Adv Sci (Weinh). 2026 Apr;13(19):e16090. [Abstract]
- Adv Sci (Weinh). 2025 Oct 27:e12895. [Abstract]
- Adv Sci (Weinh). 2024 Mar;11(10):e2303388. [Abstract]
- Mol Ther. 2024 Jun 5;32(6):1760-1778. [Abstract]
- Redox Biol. 2025 Sep 30:87:103881. [Abstract]
- Brain. 2024 Jul 25:awae241. [Abstract]
- J Control Release. 2025 Sep 30;388(Pt 1):114283. [Abstract]
- EBioMedicine. 2025 Aug 15:119:105879. [Abstract]
- Nat Cardiovasc Res. 2022 Jul;1(7):649-664. [Abstract]
- Cancer Lett. 2021 Dec 1;522:171-183. [Abstract]
- Int J Biol Sci. 2021 Jun 26;17(11):2703-2717. [Abstract]
- Cell Death Dis. 2025 Dec 7. [Abstract]
- Cell Death Dis. 2025 Mar 15;16(1):177. [Abstract]
- Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
- Biomater Res. 2023 Jul 1;27(1):63. [Abstract]
- Phytomedicine. 2026 Jan 20:153:157860. [Abstract]
- Phytomedicine. 2022 May:99:153964. [Abstract]
- Free Radic Biol Med. 2026 Mar 18:250:1-15. [Abstract]
- J Transl Med. 2025 Apr 24;23(1):471. [Abstract]
- Stem Cell Res Ther. 2025 Jul 31;16(1):416. [Abstract]
- Stem Cell Res Ther. 2025 Feb 7;16(1):55. [Abstract]
- Stem Cell Res Ther. 2024 Dec 18;15(1):471. [Abstract]
- Environ Pollut. 2021 Apr 1;274:116541. [Abstract]
- Curr Res Food Sci. 2024 Sep 21:9:100864. [Abstract]
- J Agric Food Chem. 2025 Oct 22. [Abstract]
- EMBO Rep. 2025 Jul 28. [Abstract]
- Ecotoxicol Environ Saf. 2025 Jan 8:289:117693. [Abstract]
- Ecotoxicol Environ Saf. 2021 Sep 15;221:112425. [Abstract]
- Food Biosci. 2024 Dec.
- Eur J Med Chem. 2024 Dec 15:280:116990. [Abstract]
- Mol Cell Proteomics. 2023 Jun;22(6):100551. [Abstract]
- J Ethnopharmacol. 2025 Dec 17:359:121067. [Abstract]
- Chem Biol Interact. 2026 Jan 5:423:111811. [Abstract]
- Radiother Oncol. 2024 Jan:190:110028. [Abstract]
- Commun Biol. 2024 Aug 16;7(1):1003. [Abstract]
- Commun Biol. 2022 Jun 30;5(1):643. [Abstract]
- Mech Ageing Dev. 2022 Jul:205:111688. [Abstract]
- CNS Neurosci Ther. 2023 Oct;29(10):2787-2799. [Abstract]
- Int J Mol Sci. 2022 May 11;23(10):5367. [Abstract]
- Pharmaceuticals (Basel). 2025 Mar 6;18(3):374. [Abstract]
- Int Immunopharmacol. 2025 Jan 24:148:114126. [Abstract]
- Invest Ophthalmol Vis Sci. 2024 Jul 1;65(8):1. [Abstract]
- Bioorg Chem. 2022 Oct:127:106019. [Abstract]
- Neuropharmacology. 2024 Jul 22:110090. [Abstract]
- Cancers (Basel). 2024 Aug 1;16(15):2739. [Abstract]
- Cancer Sci. 2025 Jan;116(1):152-163. [Abstract]
- Exp Neurol. 2025 Oct 6:395:115493. [Abstract]
- J Cell Mol Med. 2025 Oct;29(20):e70857. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2024 Jun;1870(5):167220. [Abstract]
- FASEB J. 2021 Feb;35(2):e21316. [Abstract]
- FASEB J. 2020 Apr;34(4):5740-5753. [Abstract]
- Process Biochem. 2024 Nov.
- Process Biochem. 2024 Oct.
- Sci Rep. 2026 Jan 17;16(1):5631. [Abstract]
- J Biol Chem. 2024 Jul 9:107543. [Abstract]
- Biocatal Agric Biotechnol. 2023 Jul.
- Heliyon. 2024 Feb 1;10(3):e25458. [Abstract]
- Food Chem Toxicol. 2024 Nov:193:115024. [Abstract]
- J Biosci Bioeng. 2025 Mar 24:S1389-1723(25)00052-0. [Abstract]
- Peptides. 2024 Jul 11:171271. [Abstract]
- Tissue Cell. 2025 Sep 15:98:103142. [Abstract]
- J Steroid Biochem Mol Biol. 2024 Jul 23:106589. [Abstract]
- Toxicol Res (Camb). 2022 Mar 28;11(2):311-324. [Abstract]
- bioRxiv. 2026 May 13.
- SSRN. 2026 Mar 16.
- bioRxiv. 2026 Jan 28:2026.01.26.701775. [Abstract]
- bioRxiv. 2025 April 18.
- SSRN. 2025 Feb 7.
- Autophagy Rep. 2025 Mar 4;4(1):2471121. [Abstract]
- bioRxiv. 2025 January 22.
- SRRN. 2024 Jul 15.
- Research Square Print. 2023 Mar 16.
- Research Square Preprint. 2021 Jun.
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IF
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IF
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IF
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1.Preparation of TMRE working solution
1.1Preparation of the stock solution
Dissolve TMRE in DMSO to obtain 5 mM of stock solution.
1.2 Preparation of TMRE working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-20 μM of working solution.
Note: Please adjust the concentration of TMRE working solution according to the actual situation.
2. Cell staining (suspended cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL.
2.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
3.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.
Note: If detection by flow cytometry, cells need to be resuspended before staining.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
576
550
Chemical Information
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CAS No. 115532-52-0
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Appearance Solid
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Molecular Weight 514.95
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Formula C26H27ClN2O7
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Color Brown to black
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SMILES
O=C(C1=CC=CC=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)OCC.O=Cl(=O)([O-])=O
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Synonyms
Tetramethylrhodamine ethyl ester perchlorate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)
Publications (77)
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Journal Impact Factor
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Most Recent
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Sci Bull
2025 May 16:S2095-9273(25)00531-6. PMID: 40473539 -
Nat Aging
Converting cell death into senescence by PARP1 inhibition improves recovery from acute oxidative injury. [Abstract]2024 May 9. PMID: 38724734 -
Autophagy
Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin. [Abstract]2023 Aug;19(8):2240-2256. PMID: 36779633 -
Autophagy
FOXG1 promotes aging inner ear hair cell survival through activation of the autophagy pathway. [Abstract]2021 Dec;17(12):4341-4362. PMID: 34006186 -
Adv Sci (Weinh)
Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline Metabolism. [Abstract]2026 Apr;13(19):e16090. PMID: 41618855 -
Adv Sci (Weinh)
ARL5B Drives Esophageal Squamous Cell Carcinoma Progression via ROCK1-SREBP1-Mediated Lipid Metabolic Reprogramming. [Abstract]2025 Oct 27:e12895. PMID: 41144804 -
Adv Sci (Weinh)
TNF-α-Induced KAT2A Impedes BMMSC Quiescence by Mediating Succinylation of the Mitophagy-Related Protein VCP. [Abstract]2024 Mar;11(10):e2303388. PMID: 38145956 -
Mol Ther
Sirt6 protects retinal ganglion cells and optic nerve from degeneration during aging and glaucoma. [Abstract]2024 Jun 5;32(6):1760-1778. PMID: 38659223 -
Redox Biol
Neutrophil extracellular traps exacerbate inflammatory arthritis by inhibiting γδ Treg cell differentiation via the AIM2 inflammasome. [Abstract]2025 Sep 30:87:103881. PMID: 41046783 -
Brain
2024 Jul 25:awae241. PMID: 39054908 -
J Control Release
Targeting polyamine metabolism induces oxidative/carbonyl stress to reinvigorate antitumor immunity in prostate cancer. [Abstract]2025 Sep 30;388(Pt 1):114283. PMID: 41038348 -
EBioMedicine
Differential regulation of FADS2 by EZH2 reveals a metabolic vulnerability in ovarian cancer treatment. [Abstract]2025 Aug 15:119:105879. PMID: 40818202 -
Nat Cardiovasc Res
2022 Jul;1(7):649-664. PMID: 36034743 -
Cancer Lett
STAT3β disrupted mitochondrial electron transport chain enhances chemosensitivity by inducing pyroptosis in esophageal squamous cell carcinoma. [Abstract]2021 Dec 1;522:171-183. PMID: 34571081 -
Int J Biol Sci
Tagitinin C induces ferroptosis through PERK-Nrf2-HO-1 signaling pathway in colorectal cancer cells. [Abstract]2021 Jun 26;17(11):2703-2717. PMID: 34345202
TMRE purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Jun 26;17(11):2703-2717. [Abstract]
When the mitochondrial membrane potential (ΔΨm) is high, TMRE gathers in the mitochondria and produces red-orange fluorescence. HCT116 cells are seeded in 96‐well plates and treated with Tagitinin C. Then cells are incubated for 30 minutes at 37 °C with TMRE (1 µM) in PBS. The excitation wavelength is 540 nm, and the emission wavelength is 595 nm.
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Cell Death Dis
FAM3A drives uncoupling of muscle lipid accumulation and insulin resistance depending on insulin receptor. [Abstract]2025 Dec 7. PMID: 41353211 -
Cell Death Dis
CKMT1 deficiency contributes to mitochondrial dysfunction and promotes intestinal epithelial cell apoptosis via reverse electron transfer-derived ROS in colitis. [Abstract]2025 Mar 15;16(1):177. PMID: 40089459 -
Cell Death Dis
Inhibition of VDAC1 oligomerization blocks cysteine deprivation-induced ferroptosis via mitochondrial ROS suppression. [Abstract]2024 Nov 9;15(11):811. PMID: 39521767 -
Biomater Res
Fe3+-binding transferrin nanovesicles encapsulating sorafenib induce ferroptosis in hepatocellular carcinoma. [Abstract]2023 Jul 1;27(1):63. PMID: 37391845 -
Phytomedicine
Anti-tumor effects of Guggulsterone in osteosarcoma: Role of SIRT3-mediated PINK1-Parkin mitophagy activation. [Abstract]2026 Jan 20:153:157860. PMID: 41650525 -
Phytomedicine
Salidroside inhibits doxorubicin-induced cardiomyopathy by modulating a ferroptosis-dependent pathway. [Abstract]2022 May:99:153964. PMID: 35180677 -
Free Radic Biol Med
DECR1 deficiency activates a lipid peroxidation-mitocytosis-mitochondrial dysfunction axis in trophoblasts to promote preeclampsia. [Abstract]2026 Mar 18:250:1-15. PMID: 41862008 -
J Transl Med
OPA1 mutations in dominant optic atrophy: domain-specific defects in mitochondrial fusion and apoptotic regulation. [Abstract]2025 Apr 24;23(1):471. PMID: 40275276 -
Stem Cell Res Ther
Inhibition of miR-615-3p enhances dentinogenesis in scaps via PVT1-mediated mitochondrial regulation. [Abstract]2025 Jul 31;16(1):416. PMID: 40745571 -
Stem Cell Res Ther
Betaine enhances SCAPs chondrogenic differentiation and promotes cartilage repair in TMJOA through WDR81. [Abstract]2025 Feb 7;16(1):55. PMID: 39920811 -
Stem Cell Res Ther
FAM96B negatively regulates FOSL1 to modulate the osteogenic differentiation and regeneration of periodontal ligament stem cells via ferroptosis. [Abstract]2024 Dec 18;15(1):471. PMID: 39696611 -
Environ Pollut
Comprehensive analysis of organophosphorus flame retardant-induced mitochondrial abnormalities: Potential role in lipid accumulation. [Abstract]2021 Apr 1;274:116541. PMID: 33529899 -
Curr Res Food Sci
Energy insufficiency induced by high purine diet: Catalysts for renal impairment in hyperuricemia nephropathy rat model. [Abstract]2024 Sep 21:9:100864. PMID: 39381132 -
J Agric Food Chem
Profiling Class-Wide Bioactivities of Flavonoids While Minimizing Compound-Specific Effects Using an Equimolar Mixture Strategy. [Abstract]2025 Oct 22. PMID: 41126446 -
EMBO Rep
The E3 ubiquitin ligase adaptor KLHL8 targets ZAR1 to regulate maternal mRNA degradation in oocytes. [Abstract]2025 Jul 28. PMID: 40721554 -
Ecotoxicol Environ Saf
BPZ inhibits early mouse embryonic development by disrupting maternal-to-zygotic transition and mitochondrial function. [Abstract]2025 Jan 8:289:117693. PMID: 39788034 -
Ecotoxicol Environ Saf
Multiplexed quantitative evaluation on mitochondrial toxicity of tris (2,3-dibromopropyl) phosphate in hepatocyte. [Abstract]2021 Sep 15;221:112425. PMID: 34146984
TMRE purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2021 Sep 15;221:112425. [Abstract]
AML-12 cells were treated with TDBPP, then TMRE (100 nM) was added to each well. Cells were stained for 30 min. The red fluorescence stained by TMRE represented mitochondria.
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Eur J Med Chem
Coumarin-Quinazolinone based photosensitizers: Mitochondria and endoplasmic reticulum targeting for enhanced phototherapy via different cell death pathways. [Abstract]2024 Dec 15:280:116990. PMID: 39442335 -
Mol Cell Proteomics
Sulconazole induces PANoptosis by triggering oxidative stress and inhibiting glycolysis to increase radiosensitivity in esophageal cancer. [Abstract]2023 Jun;22(6):100551. PMID: 37076047 -
J Ethnopharmacol
Cannabidiol attenuates the LPS/D-Galactosamine-induced acute liver injury by inhibiting parkin-mediated ubiquitination of MFN2. [Abstract]2025 Dec 17:359:121067. PMID: 41419044 -
Chem Biol Interact
Cigarette smoke exposure diminishes ovarian reserve in mice by regulating granulosa cells redox homeostasis imbalance through Wnt10b-ERβ feedback loop. [Abstract]2026 Jan 5:423:111811. PMID: 41202950 -
Radiother Oncol
Mitophagy induction improves salivary gland stem/progenitor cell function by reducing senescence after irradiation. [Abstract]2024 Jan:190:110028. PMID: 38007043 -
Commun Biol
Rhein targets macrophage SIRT2 to promote adipose tissue thermogenesis in obesity in mice. [Abstract]2024 Aug 16;7(1):1003. PMID: 39152196 -
Commun Biol
C. elegans monitor energy status via the AMPK pathway to trigger innate immune responses against bacterial pathogens. [Abstract]2022 Jun 30;5(1):643. PMID: 35773333 -
Mech Ageing Dev
Inhibition of Cpt1a alleviates oxidative stress-induced chondrocyte senescence via regulating mitochondrial dysfunction and activating mitophagy. [Abstract]2022 Jul:205:111688. PMID: 35728631 -
CNS Neurosci Ther
The neurotrophic activities of brain-derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling. [Abstract]2023 Oct;29(10):2787-2799. PMID: 37101380 -
Int J Mol Sci
Cannabidiol Enhances Microglial Beta-Amyloid Peptide Phagocytosis and Clearance via Vanilloid Family Type 2 Channel Activation. [Abstract]2022 May 11;23(10):5367. PMID: 35628181 -
Pharmaceuticals (Basel)
Gambogenic Acid Suppresses Malignant Progression of Non-Small Cell Lung Cancer via GCH1-Mediated Ferroptosis. [Abstract]2025 Mar 6;18(3):374. PMID: 40143150 -
Int Immunopharmacol
Anti-osteosarcoma activity of Corynoline via Src/JNK signaling-mediated cell cycle arrest and mitochondrial apoptosis. [Abstract]2025 Jan 24:148:114126. PMID: 39862636 -
Invest Ophthalmol Vis Sci
Protective Effect of Nicotinamide Riboside on Glucocorticoid-Induced Glaucoma: Mitigating Mitochondrial Damage and Extracellular Matrix Deposition. [Abstract]2024 Jul 1;65(8):1. PMID: 38949632 -
Bioorg Chem
A novel Gboxin analog induces OXPHOS inhibition and mitochondrial dysfunction-mediated apoptosis in diffuse large B-cell lymphoma. [Abstract]2022 Oct:127:106019. PMID: 35849895 -
Neuropharmacology
Neuronostatin regulates neuronal function and energetic metabolism in Alzheimer's disease in a GPR107-dependent manner. [Abstract]2024 Jul 22:110090. PMID: 39048031 -
Cancers (Basel)
Bufalin Suppresses Head and Neck Cancer Development by Modulating Immune Responses and Targeting the β-Catenin Signaling Pathway. [Abstract]2024 Aug 1;16(15):2739. PMID: 39123466 -
Cancer Sci
Noncanonical TCA cycle fosters canonical TCA cycle and mitochondrial integrity in acute myeloid leukemia. [Abstract]2025 Jan;116(1):152-163. PMID: 39479926 -
Exp Neurol
Physical exercise protects neurons from energy deficit and improves cognitive function by upregulating astrocytic Slc2a1 in Alzheimer's disease. [Abstract]2025 Oct 6:395:115493. PMID: 41061790 -
J Cell Mol Med
Ferritinophagy Is Involved in the Advanced Glycation End Products-Induced Ferroptosis of Vascular Endothelial Cells in Diabetes Mellitus. [Abstract]2025 Oct;29(20):e70857. PMID: 41139808 -
Biochim Biophys Acta Mol Basis Dis
Targeted inhibition of branched-chain amino acid metabolism drives apoptosis of glioblastoma by facilitating ubiquitin degradation of Mfn2 and oxidative stress. [Abstract]2024 Jun;1870(5):167220. PMID: 38718847 -
FASEB J
Butyrate drives the acetylation of histone H3K9 to activate steroidogenesis through PPARγ and PGC1α pathways in ovarian granulosa cells. [Abstract]2021 Feb;35(2):e21316. PMID: 33433947 -
FASEB J
HIF-1α/Actl6a/H3K9ac axis is critical for pluripotency and lineage differentiation of human induced pluripotent stem cells. [Abstract]2020 Apr;34(4):5740-5753. PMID: 32112486
TMRE purchased from MedChemExpress. Usage Cited in: FASEB J. 2020 Apr;34(4):5740-5753. [Abstract]
Cells were rinsed with D-PBS, and then incubated with TMRE (50 nM) at 37°C for 20 minutes.
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Sci Rep
CDC25C downregulation suppresses HCC growth via mitochondrial stress-induced autophagy and apoptosis. [Abstract]2026 Jan 17;16(1):5631. PMID: 41547943 -
J Biol Chem
BL-918 activates PINK1/Parkin signaling pathway to ameliorate the progression of Parkinson's disease. [Abstract]2024 Jul 9:107543. PMID: 38992440 -
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Heliyon
Equisetin inhibits adiposity through AMPK-dependent regulation of brown adipocyte differentiation. [Abstract]2024 Feb 1;10(3):e25458. PMID: 38327434 -
Food Chem Toxicol
Methylmercury chloride inhibits meiotic maturation of mouse oocytes in vitro by disrupting the cytoskeleton. [Abstract]2024 Nov:193:115024. PMID: 39341490 -
J Biosci Bioeng
Polyvinyl alcohol, N-acetylcysteine, and methyl-β-cyclodextrin exhibit albumin functions in natural killer cell culture. [Abstract]2025 Mar 24:S1389-1723(25)00052-0. PMID: 40133146 -
Peptides
Tirzepatide shows neuroprotective effects via regulating brain glucose metabolism in APP/PS1 mice. [Abstract]2024 Jul 11:171271. PMID: 39002758 -
Tissue Cell
2025 Sep 15:98:103142. PMID: 40974710 -
J Steroid Biochem Mol Biol
Free fatty acids induce bile acids overproduction and oxidative damage of bovine hepatocytes via inhibiting FXR/SHP signaling. [Abstract]2024 Jul 23:106589. PMID: 39053701 -
Toxicol Res (Camb)
Rational deuteration of dronedarone attenuates its toxicity in human hepatic HepG2 cells. [Abstract]2022 Mar 28;11(2):311-324. PMID: 35510231 -
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bioRxiv
2026 Jan 28:2026.01.26.701775. PMID: 41659589 -
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Autophagy Rep
Loss of nuclear envelope bud formation leads to mitophagy initiation in Drosophila muscles. [Abstract]2025 Mar 4;4(1):2471121. PMID: 40395995 -
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Solvent & Solubility
DMSO : 27.78 mg/mL (53.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
The entire experiment should be performed at room temperature because temperature will directly impact mitochondrial transmembrane potential and TMRE staining. Cells should never be placed, centrifuged, incubated, or washed at 4°C or have ice-cold buffers or media added. Treat the cells with a cytotoxic stimulus. Harvest cells and resuspend at 5×105 cells/mL in culture medium containing 150 nM TMRE. Incubate for 5 min at room temperature in the dark. Add Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (5 μM final concentration) to an aliquot of untreated cells and incubate for 5 min at room temperature in the dark. Turn on the appropriate laser on the flow cytometer. Set up a histogram plot to detect TMRE using log scale[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Crowley LC, et al. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot087361. [Content Brief]
[2]. Crowley LC, et al. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec 1;2016(12):pdb.prot087361. [Content Brief]
[3]. Bantseev V, et al. Confocal laser scanning microscopy imaging of dynamic TMRE movement in the mitochondria of epithelial and superficial cortical fiber cells of bovine lenses. Mol Vis. 2005 Jul 14;11:518-23. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9419 mL | 9.7097 mL | 19.4194 mL | 48.5484 mL |
| 5 mM | 0.3884 mL | 1.9419 mL | 3.8839 mL | 9.7097 mL | |
| 10 mM | 0.1942 mL | 0.9710 mL | 1.9419 mL | 4.8548 mL | |
| 15 mM | 0.1295 mL | 0.6473 mL | 1.2946 mL | 3.2366 mL | |
| 20 mM | 0.0971 mL | 0.4855 mL | 0.9710 mL | 2.4274 mL | |
| 25 mM | 0.0777 mL | 0.3884 mL | 0.7768 mL | 1.9419 mL | |
| 30 mM | 0.0647 mL | 0.3237 mL | 0.6473 mL | 1.6183 mL | |
| 40 mM | 0.0485 mL | 0.2427 mL | 0.4855 mL | 1.2137 mL | |
| 50 mM | 0.0388 mL | 0.1942 mL | 0.3884 mL | 0.9710 mL |