Forsythiaside A
Based on 9 publication(s) in Google Scholar
Forsythiaside A is an orally active phenylethanoid glycoside isolated from the dried fruits of Forsythia suspensa. Forsythiaside A is also an inhibitor of COX-2 and has anti-inflammatory, antioxidant and neuroprotective effects. Forsythiaside A prevents neuroinflammation and apoptosis caused by Aβ25-35 damage and may be used in Alzheimer's disease (AD) research. Forsythiaside A also activates the Nrf2/HO-1 signaling pathway and inhibits OVA-induced asthma in mice. Forsythiaside A inhibits the interaction between KLRB1 and CLEC2D.
For research use only. We do not sell to patients.
- Purity: 99.79%
- CAS No.: 79916-77-1
- Formula: C29H36O15
- Molecular Weight:624.59
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Forsythiaside A
More- Phytomedicine. 2024 Dec:135:156052. [Abstract]
- CNS Neurosci Ther. 2026 Apr;32(4):e70867. [Abstract]
- Pharmaceuticals (Basel). 2026 May 29;19(6):852. [Abstract]
- Int Immunopharmacol. 2024 Mar 10:129:111650. [Abstract]
- J Nutr Biochem. 2026 May 9:157:110407. [Abstract]
- Food Sci Nutr. 2025 Sep 29;13(10):e70991. [Abstract]
- Exp Cell Res. 2026 Aug 1;461(1):115097. [Abstract]
- Chem Biol Drug Des. 2025 Mar;105(3):e70083. [Abstract]
- Kaohsiung J Med Sci. 2025 Nov 28:e70144. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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Cell Imaging/Staining
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IF
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Histological Imaging/Staining
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Neutrophil | IC50 |
>10 μg/mL
Compound: 15
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Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pre-incubated for 5 mins before fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-pnitroanilide as substrate
Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pre-incubated for 5 mins before fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-pnitroanilide as substrate
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[PMID: 28218000] |
| Neutrophil | IC50 |
1.4 μg/mL
Compound: 15
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Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation by measuring superoxide dismutase SOD-inhibitable ferricytochrome c reduction incubated for 5 mins before fMLP/CB stimulation for 3 mins
Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation by measuring superoxide dismutase SOD-inhibitable ferricytochrome c reduction incubated for 5 mins before fMLP/CB stimulation for 3 mins
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[PMID: 28218000] |
Forsythiaside A inhibits COX-2 at 30 μM, with inhibitory rate of 72%[2].
Forsythiaside A (80 μM; 30 min) increases the contents of endogenous 2-AG, via decreasing MAGL expressions in Aβ25-35 (5 μM; 2 h) treated organotypic hippoc ampal slice[2].
Forsythiaside A prevents Aβ25-35-induced neuroinflammation in a CB1R-dependent manner[2].
Forsythiaside A (2.5-10 μg/mL; 12 h) inhibits Nrf2 signaling pathway in lung epithelial cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 79916-77-1
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Appearance Solid
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Molecular Weight 624.59
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Formula C29H36O15
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Color Off-white to yellow
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SMILES
O[C@@H]1[C@@H](O)[C@@H](O)[C@H](OC[C@@H]2[C@@H](OC(/C=C/C3=CC=C(O)C(O)=C3)=O)[C@H](O)[C@@H](O)[C@H](OCCC4=CC(O)=C(O)C=C4)O2)O[C@H]1C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (9)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Forsythoside A mitigates osteoarthritis and inhibits chondrocyte senescence by promoting mitophagy and suppressing NLRP3 inflammasome via the Nrf2 pathway. [Abstract]2024 Dec:135:156052. PMID: 39383631
Forsythiaside A purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Dec:135:156052. [Abstract]
The CCK8 method was used to detect chondrocyte activity after treatment with different concentrations of Forsythiaside A (FTA) (5, 10, 20, 40, 80 μM).
Forsythiaside A purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Dec:135:156052. [Abstract]
Western blot analysis of COL2, MMP13, p21, and TNF-α protein expression with 10 or 20 μM Forsythiaside A (FTA) or mitoquinone (MitoQ) for 2 h before stimulation with TBHP (100 μM) for 24 h.
Forsythiaside A purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Dec:135:156052. [Abstract]
Representative images of Alcian blue staining with 10 or 20 μM Forsythiaside A (FTA) or mitoquinone (MitoQ) for 2 h before stimulation with TBHP (100 μM) for 24 h.
Forsythiaside A purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Dec:135:156052. [Abstract]
DNA damage in chondrocytes was detected via rH2AX immunofluorescence and observed by fluorescence microscopy with 10 or 20 μM Forsythiaside A (FTA) or mitoquinone (MitoQ) for 2 h before stimulation with TBHP (100 μM) for 24 h.
Forsythiaside A purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2024 Dec:135:156052. [Abstract]
Safranin O-Fast Green and H&E staining of mouse knee joints treated with Forsythiaside A (FTA) (30 mg/kg, i.g.).
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CNS Neurosci Ther
Forsythoside A Alleviates Neuroinflammatory Damage via Inhibiting TLR4/NF-κB/NLRP3 Activation-Induced Astrocyte Pyroptosis in Cerebral Ischemia-Reperfusion. [Abstract]2026 Apr;32(4):e70867. PMID: 41948887 -
Pharmaceuticals (Basel)
Forsythoside A Attenuates High-Fat Diet-Induced Obesity by Regulating Thermogenesis and Browning of White Adipose Tissue Through Activation of the AMPK Signaling Pathway. [Abstract]2026 May 29;19(6):852. PMID: 42356469 -
Int Immunopharmacol
Forsythiaside A suppresses renal fibrosis and partial epithelial-mesenchymal transition by targeting THBS1 through the PI3K/AKT signaling pathway. [Abstract]2024 Mar 10:129:111650. PMID: 38342062 -
J Nutr Biochem
Forsythiaside A attenuates intervertebral disc degeneration by suppressing PI3K/AKT/NF-κB-mediated NLRP3 inflammasome activation and pyroptosis. [Abstract]2026 May 9:157:110407. PMID: 42114575 -
Food Sci Nutr
Forsythiaside A Suppresses Ferroptosis and Mitigates Type 2 Diabetes Osteoporosis Through the NRF2/GPX4 Axis. [Abstract]2025 Sep 29;13(10):e70991. PMID: 41036508 -
Exp Cell Res
Forsythiaside A attenuates sepsis-induced acute lung injury by suppressing EGR1/GLS2-mediated ferroptosis-associated lipid peroxidation. [Abstract]2026 Aug 1;461(1):115097. PMID: 42235787 -
Chem Biol Drug Des
Forsythiaside A Ameliorates Oxidative Damage Caused by Cerebral Ischemia Through the Nrf2/HO-1 Signaling Pathway. [Abstract]2025 Mar;105(3):e70083. PMID: 40035314 -
Kaohsiung J Med Sci
Forsythiaside A Alleviates Kidney Injury and Intestinal Epithelium Dysfunction in IgA Nephropathy by Inhibiting TLR4/NF-κB Signaling. [Abstract]2025 Nov 28:e70144. PMID: 41313694
Solvent & Solubility
DMSO : 100 mg/mL (160.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (160.11 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Wang Y, et al. Forsythiaside A Exhibits Anti-inflammatory Effects in LPS-Stimulated BV2 Microglia Cells Through Activation of Nrf2/HO-1 Signaling Pathway. Neurochem Res. 2016 Apr;41(4):659-65. [Content Brief]
[2]. Chen L, et al. Forsythiaside prevents β-amyloid-induced hippocampal slice injury by upregulating 2-arachidonoylglycerol via cannabinoid receptor 1-dependent NF-κB pathway. Neurochem Int. 2019 May;125:57-66. [Content Brief]
[3]. Qian J, et al. Protective effect of forsythiaside A on OVA-induced asthma in mice. Eur J Pharmacol. 2017 Oct 5;812:250-255. [Content Brief]
[4]. Yaoyao Zhu, et al. Comprehensive pan-cancer analysis of KLRB1-CLEC2D pair and identification of small molecule inhibitors to disrupt their interaction. Int Immunopharmacol. 2024 Oct 25:140:112908. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.6011 mL | 8.0053 mL | 16.0105 mL | 40.0263 mL |
| 5 mM | 0.3202 mL | 1.6011 mL | 3.2021 mL | 8.0053 mL | |
| 10 mM | 0.1601 mL | 0.8005 mL | 1.6011 mL | 4.0026 mL | |
| 15 mM | 0.1067 mL | 0.5337 mL | 1.0674 mL | 2.6684 mL | |
| 20 mM | 0.0801 mL | 0.4003 mL | 0.8005 mL | 2.0013 mL | |
| 25 mM | 0.0640 mL | 0.3202 mL | 0.6404 mL | 1.6011 mL | |
| 30 mM | 0.0534 mL | 0.2668 mL | 0.5337 mL | 1.3342 mL | |
| 40 mM | 0.0400 mL | 0.2001 mL | 0.4003 mL | 1.0007 mL | |
| 50 mM | 0.0320 mL | 0.1601 mL | 0.3202 mL | 0.8005 mL | |
| 60 mM | 0.0267 mL | 0.1334 mL | 0.2668 mL | 0.6671 mL | |
| 80 mM | 0.0200 mL | 0.1001 mL | 0.2001 mL | 0.5003 mL | |
| 100 mM | 0.0160 mL | 0.0801 mL | 0.1601 mL | 0.4003 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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