m-PEG2000-NHS ester
Based on 1 Customer Validation
m-PEG2000-NHS ester (mPEG2000-SC) is a reagent with both cell adhesion inhibition and peptide conjugation functions. The NHS ester group of m-PEG2000-NHS ester forms stable amide bonds with primary amine-containing molecules (e.g., the N-terminus of MMP-2-cleavable octapeptide) to generate mPEG-peptide intermediates for liposome surface modification. When m-PEG2000-NHS ester is immobilized on a cystamine-modified gold surface, it can construct an in vitro model for cell adhesion kinetic studies, and higher PEG density and thicker layers correlate with lower cell adhesion rates. m-PEG2000-NHS ester can synthesize MMP-2-responsive PEGylated lipid conjugates to achieve MMP-triggered dePEGylation in the tumor microenvironment. m-PEG2000-NHS ester can be used in studies related to colon cancer and other conditions.
For research use only. We do not sell to patients.
- Purity: 95.89%
- CAS No.: 92451-01-9
- Formula: (C2H4O)nC7H9NO5
- Molecular Weight:2000 (Average)
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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MMP-2 |
m-PEG2000-NHS ester (10 μL/h; 8 h) creates a PEG thickness gradient (0 to 16 nm) that reduces the adhesion rate of BAVEC-1 cells, with near-zero adhesion rate at the 16 nm thick gradient center when delivered into a 3% agarose gel overlay on cystamine-coated gold substrates[1].
m-PEG2000-NHS ester (1:5 molar ratio; overnight, 2 h, 48 h) successfully forms an amide bond with the N-terminus of an MMP-2-cleavable octapeptide, which is then conjugated to DOPE to create an MMP-2-responsive PEG-lipid conjugate[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 92451-01-9
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Appearance Solid
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Molecular Weight 2000 (Average)
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Formula (C2H4O)nC7H9NO5
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Color White to off-white
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SMILES
COCCOCC(ON1C(CCC1=O)=O)=O.[n]
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Synonyms
mPEG2000-SC; mPEG2000-Succinimidyl ester
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Mougin K, et al. Construction of a tethered poly(ethylene glycol) surface gradient for studies of cell adhesion kinetics. Langmuir. 2005;21(11):4809-4812. [Content Brief]
[2]. Askarizadeh A, et al. Surface-modified cationic liposomes with a matrix metalloproteinase-degradable polyethylene glycol derivative improved doxorubicin delivery in murine colon cancer. J Liposome Res. 2024;34(2):221-238. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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