Dulcoside A
Based on 1 Customer Validation
Dulcoside A is a glycoside that can be isolated from Stevia rebaudiana that elicits sweet and bitter taste through activation of TAS1R. Dulcoside A also exhibits anti-inflammatory activity.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 64432-06-0
- Formula: C38H60O17
- Molecular Weight:788.87
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Dulcoside A produces sweetness by activating sweet taste receptors hTAS1R2/hTAS1R3 with a sweet taste threshold of 32.9 μM. Dulcoside A also activates bitter taste receptors hTAS2R4 and hTAS2R14, thereby producing bitter taste with a sweet taste threshold of 49 μM. Dulcoside A activates the hTAS1R2/hTAS1R3, hTAS2R4 and hTAS2R14 with the threshold concentrations of 38.8 μM, 200 μM and 50 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 64432-06-0
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Appearance Solid
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Molecular Weight 788.87
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Formula C38H60O17
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Color White to off-white
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SMILES
C[C@]12[C@@]3([H])[C@@](C4)(CC[C@]1([H])[C@@](C)(CCC2)C(O[C@@H]5O[C@@H]([C@@H](O)[C@H](O)[C@H]5O)CO)=O)C[C@@](C4=C)(O[C@@]6([H])[C@@H]([C@H]([C@H](O)[C@@H](CO)O6)O)O[C@@]7([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O7)O)O)CC3
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (126.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.17 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.17 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Hellfritsch C, et al., Human psychometric and taste receptor responses to steviol glycosides. J Agric Food Chem. 2012 Jul 11;60(27):6782-93. [Content Brief]
[2]. Yasukawa K, et al., Inhibitory effect of stevioside on tumor promotion by 12-O-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin. Biol Pharm Bull. 2002 Nov;25(11):1488-90. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2676 mL | 6.3382 mL | 12.6764 mL | 31.6909 mL |
| 5 mM | 0.2535 mL | 1.2676 mL | 2.5353 mL | 6.3382 mL | |
| 10 mM | 0.1268 mL | 0.6338 mL | 1.2676 mL | 3.1691 mL | |
| 15 mM | 0.0845 mL | 0.4225 mL | 0.8451 mL | 2.1127 mL | |
| 20 mM | 0.0634 mL | 0.3169 mL | 0.6338 mL | 1.5845 mL | |
| 25 mM | 0.0507 mL | 0.2535 mL | 0.5071 mL | 1.2676 mL | |
| 30 mM | 0.0423 mL | 0.2113 mL | 0.4225 mL | 1.0564 mL | |
| 40 mM | 0.0317 mL | 0.1585 mL | 0.3169 mL | 0.7923 mL | |
| 50 mM | 0.0254 mL | 0.1268 mL | 0.2535 mL | 0.6338 mL | |
| 60 mM | 0.0211 mL | 0.1056 mL | 0.2113 mL | 0.5282 mL | |
| 80 mM | 0.0158 mL | 0.0792 mL | 0.1585 mL | 0.3961 mL | |
| 100 mM | 0.0127 mL | 0.0634 mL | 0.1268 mL | 0.3169 mL |
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