Cat. No.: HY-N0346A

(E)-Ethyl p-methoxycinnamate

Customer Review

Based on 1 Customer Validation

(E)-Ethyl p-methoxycinnamate is a natural product found in Kaempferia galangal with anti-inflammatory, anti-neoplastic and anti-microbial effects. (E)-Ethyl p-methoxycinnamate inhibits COX-1 and COX-2 in vitro with IC₅₀s of 1.12 and 0.83 μM, respectively.

For research use only. We do not sell to patients.

Purity: 99.26%
CAS No.: 24393-56-4
Formula: C₁₂H₁₄O₃
Molecular Weight:206.24

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

(E)-Ethyl p-methoxycinnamate Chemical Structure

CAS No.: 24393-56-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

COX-1

1.12 μM (IC₅₀)

COX-2

0.83 μM (IC₅₀)

All COX Isoforms :

All Caspase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	31.29 μM Compound: 6	Cytotoxicity against human A375 cells by MTT assay Cytotoxicity against human A375 cells by MTT assay	[PMID: 19782567]
HL-60	IC₅₀	29.64 μM Compound: 6	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 19782567]
THP-1	IC₅₀	33.52 μM Compound: 6	Cytotoxicity against human THP1 cells by MTT assay Cytotoxicity against human THP1 cells by MTT assay	[PMID: 19782567]

In Vitro

(E)-Ethyl p-methoxycinnamate exhibits moderate inhibitory activity against α-glucosidase with an IC₅₀ of 50 μM^[2].
(E)-Ethyl p-methoxycinnamate (20-40 μM, 17 h) inhibits the migration and tubular structure formation in HUVECs without significant cytotoxicity^[3].
(E)-Ethyl p-methoxycinnamate (100-200 μg/mL, 48 h) exhibits cytotoxicity in cancer cells (IC₅₀s for PC-3, HCT116, K562 and MCF-7 is 39, 42.1, 57.5, and 77.5 μg/mL, respectively), inhibits the colony formation of HCT116, decreases the mitochondrial membrane potential, and induces apoptosis through caspase-3/7/8/9 activation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[4]

Cell Line:

PC-3, HCT116, K562 and MCF-7
Concentration:

100-200 μg/mL
Incubation Time:

48 h
Result:

Reduced cell numbers.

Apoptosis Analysis^[4]

Cell Line:

HCT116
Concentration:

50-200 μg/mL
Incubation Time:

8 h
Result:

Induced chromatin condensation.

Cell Migration Assay ^[3]

Cell Line:

HUVECs
Concentration:

20-40 μM
Incubation Time:

3 h
Result:

Inhibited FBS-induced cell migration.

In Vivo

(E)-Ethyl p-methoxycinnamate (40-200 μM, sc, single dose) inhibits bFGF and heparin-induced angiogenesis, exhibits antiangiogenic activity in mouse matrigel plug models^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mouse matrigel plug models^[3]
Dosage:

40-200 μM
Administration:

sc, single dose
Result:

Inhibited angiogenesis.

Chemical Information

CAS No. 24393-56-4
Appearance <49°C Solid, >49°C Liquid,
Molecular Weight 206.24
Formula C₁₂H₁₄O₃
Color Colorless to light yellow
SMILES

O=C(OCC)/C=C/C1=CC=C(OC)C=C1
Structure Classification
- Phenylpropanoids
- Simple Phenylpropanols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (484.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8487 mL

24.2436 mL

48.4872 mL

5 mM

0.9697 mL

4.8487 mL

9.6974 mL

10 mM

0.4849 mL

2.4244 mL

4.8487 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (12.12 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (12.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Umar MI, et al. Bioactivity-guided isolation of ethyl-p-methoxycinnamate, an anti-inflammatory constituent, from Kaempferia galangal L. extracts. Molecules. 2012 Jul 23;17(7):8720-34. [Content Brief]

[2]. Adisakwattana S, et al., Structure-activity relationships of trans-cinnamic acid derivatives on alpha-glucosidase inhibition. Bioorg Med Chem Lett. 2004 Jun 7;14(11):2893-6. [Content Brief]

[3]. He ZH, et al., Antiangiogenic effects and mechanisms of trans-ethyl p-methoxycinnamate from Kaempferia galanga L. J Agric Food Chem. 2012 Nov 14;60(45):11309-17. [Content Brief]

[4]. Umar M I, et al. Cytotoxic and pro-apoptotic properties of Ethyl-P-methoxycinnamate and its hydrophilic derivative potassium-P-methoxycinnamate[J]. Chemistry Africa, 2018, 1: 87-95.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.8487 mL	24.2436 mL	48.4872 mL	121.2180 mL
	5 mM	0.9697 mL	4.8487 mL	9.6974 mL	24.2436 mL
	10 mM	0.4849 mL	2.4244 mL	4.8487 mL	12.1218 mL
	15 mM	0.3232 mL	1.6162 mL	3.2325 mL	8.0812 mL
	20 mM	0.2424 mL	1.2122 mL	2.4244 mL	6.0609 mL
	25 mM	0.1939 mL	0.9697 mL	1.9395 mL	4.8487 mL
	30 mM	0.1616 mL	0.8081 mL	1.6162 mL	4.0406 mL
	40 mM	0.1212 mL	0.6061 mL	1.2122 mL	3.0304 mL
	50 mM	0.0970 mL	0.4849 mL	0.9697 mL	2.4244 mL
	60 mM	0.0808 mL	0.4041 mL	0.8081 mL	2.0203 mL
	80 mM	0.0606 mL	0.3030 mL	0.6061 mL	1.5152 mL
	100 mM	0.0485 mL	0.2424 mL	0.4849 mL	1.2122 mL

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(E)-Ethyl p-methoxycinnamate

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All COX Isoforms

All Caspase Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(E)-Ethyl p-methoxycinnamate Related Classifications

HY-N0346A Related Classifications