eIF4A3-IN-2
Based on 1 publication(s) in Google Scholar
eIF4A3-IN-2 is a highly selective and noncompetitive eukaryotic initiation factor 4A-3 (eIF4A3) inhibitor with an IC50 of 110 nM.
For research use only. We do not sell to patients.
- Purity: 99.70%
- CAS No.: 2095677-20-4
- Formula: C25H19Br2ClN4O2
- Molecular Weight:602.70
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) eIF4A3-IN-2
More
Biological Activity
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eIF4 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | GI50 |
3.06 μM
Compound: 1a
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Growth inhibition of human HCT116 cells after 3 days by CellTiter-Glo luminescence assay
Growth inhibition of human HCT116 cells after 3 days by CellTiter-Glo luminescence assay
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[PMID: 29057054] |
eIF4A3-IN-2 (compound 2) binds to the allosteric region in eIF4A3 and inhibits in vitro ATPase, helicase, and cellular nonsense-mediated RNA decay (NMD) activities[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293T cells (transfected with the NMD reporter) luciferase assay
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Concentration:0.3, 1, 3, or 10 μM
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Incubation Time:3 or 6 h
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Result:eIF4A3-IN-2 induces an approximately 3.2-fold increase in luciferase activity, indicating that NMD is inhibited by compound 2.
Chemical Information
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CAS No. 2095677-20-4
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Appearance Solid
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Molecular Weight 602.70
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Formula C25H19Br2ClN4O2
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Color White to light yellow
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SMILES
O=C(C1=CC=C(Br)C=C1)N2CCN(C(C3=C4N(C=C(Br)C=C4)N=C3)=O)C[C@@H]2C5=CC=C(Cl)C=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Nucleic Acids Res
An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity. [Abstract]2022 Nov 28;50(21):12462-12479. PMID: 36416264
Solvent & Solubility
DMSO : 100 mg/mL (165.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (2.77 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6592 mL | 8.2960 mL | 16.5920 mL | 41.4800 mL |
| 5 mM | 0.3318 mL | 1.6592 mL | 3.3184 mL | 8.2960 mL | |
| 10 mM | 0.1659 mL | 0.8296 mL | 1.6592 mL | 4.1480 mL | |
| 15 mM | 0.1106 mL | 0.5531 mL | 1.1061 mL | 2.7653 mL | |
| 20 mM | 0.0830 mL | 0.4148 mL | 0.8296 mL | 2.0740 mL | |
| 25 mM | 0.0664 mL | 0.3318 mL | 0.6637 mL | 1.6592 mL | |
| 30 mM | 0.0553 mL | 0.2765 mL | 0.5531 mL | 1.3827 mL | |
| 40 mM | 0.0415 mL | 0.2074 mL | 0.4148 mL | 1.0370 mL | |
| 50 mM | 0.0332 mL | 0.1659 mL | 0.3318 mL | 0.8296 mL | |
| 60 mM | 0.0277 mL | 0.1383 mL | 0.2765 mL | 0.6913 mL | |
| 80 mM | 0.0207 mL | 0.1037 mL | 0.2074 mL | 0.5185 mL | |
| 100 mM | 0.0166 mL | 0.0830 mL | 0.1659 mL | 0.4148 mL |
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