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Cat. No.: HY-147520

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FAK-IN-5

FAK-IN-5 (Compound 8l) is a FAK signaling inhibitor. FAK-IN-5 induces cell apoptosis and autophagy.

For research use only. We do not sell to patients.

CAS No.: 2408317-70-2
Formula: C₂₉H₂₉ClF₃N₃O₄
Molecular Weight:576.01

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

FAK-IN-5 Chemical Structure

CAS No.: 2408317-70-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

FAK-IN-5 (Compound 8l) is a FAK signaling inhibitor. FAK-IN-5 induces cell apoptosis and autophagy^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
CCRF-CEM	GI₅₀	7.9 μM Compound: 8l	Antiproliferative activity against human CEM cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay Antiproliferative activity against human CEM cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay	[PMID: 31931341]
G-361	GI₅₀	6.3 μM Compound: 8l	Antiproliferative activity against human G361 cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay Antiproliferative activity against human G361 cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay	[PMID: 31931341]
HCT-116	GI₅₀	5.3 μM Compound: 8l	Antiproliferative activity against human HCT116 cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay Antiproliferative activity against human HCT116 cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay	[PMID: 31931341]
K562	GI₅₀	6.3 μM Compound: 8l	Antiproliferative activity against human K562 cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay Antiproliferative activity against human K562 cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay	[PMID: 31931341]
MCF7	GI₅₀	5.5 μM Compound: 8l	Antiproliferative activity against human MCF7 cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition measured after 72 hrs by calcein AM dye-based fluorescence assay	[PMID: 31931341]

In Vitro

FAK-IN-5 (Compound 8l) (0-50 µM, 72 h) shows antiproliferative activity against cancer cells^[1].
FAK-IN-5 (Compound 8l) causes cell detachment^[1].
FAK-IN-5 (Compound 8l) (0-25 µM, 24 h) induces autophagy and apoptosis in HCT-116 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

K562, CEM, G361, MCF-7 and HCT-116
Concentration:

0-50 µM
Incubation Time:

72 h
Result:

Showed antiproliferative activity with IC₅₀ values of 6.3, 7.9, 6.3, 5.5 and 5.3 µM against K562, CEM, G361, MCF-7 and HCT-116 cells, respectively.

Western Blot Analysis^[1]

Cell Line:

HCT-116
Concentration:

1.25, 2.5, 5, 7, 10, 11, 17 and 25 µM
Incubation Time:

1, 3, 5, 7 and 24 h
Result:

Caused the dephosphorylation of FAK, p130Cas and paxillin in a dose-dependent manner. The dephosphorylation of FAK at Y397 was observed at a much earlier time point. Detected the fragments of activated caspase-7 as well as the dose-dependent cleavage of poly (ADP-ribose) polymerase (PARP).

Chemical Information

CAS No. 2408317-70-2
Molecular Weight 576.01
Formula C₂₉H₂₉ClF₃N₃O₄
SMILES

CC(C[C@@H](C(N[C@H](C(NC1=CC=C(C=C1)C(F)(F)F)=O)CC2=CC=CC=C2)=O)NC(C3=CC(Cl)=CC=C3O)=O)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jorda R, et al. Novel modified leucine and phenylalanine dipeptides modulate viability and attachment of cancer cells. Eur J Med Chem. 2020 Feb 15;188:112036. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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