Cat. No.: HY-101892

DiBAC4(3)

18 Cited Publications

Customer Review

Based on 18 publication(s) in Google Scholar

DiBAC4(3) is a voltage-sensitive fluorescent dye (λ_ex=490 nm, λ_em=505 nm). DiBAC4(3) can be used to measure cell membrane potential.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 70363-83-6
Formula: C₂₇H₄₀N₄O₆
Molecular Weight:516.63

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

DiBAC4(3) Chemical Structure

CAS No.: 70363-83-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (18)
Solvent & Solubility
- In Vitro
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

DiBAC4(3) is a voltage-sensitive fluorescent dye (λ_ex=490 nm, λ_em=505 nm). DiBAC4(3) can be used to measure cell membrane potential.

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10-40 mM.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 10-40 μM with Hanks and 20 mM Hepes buffer (HHBS) or other buffer, such as pH 7 with 0.04% to 0.08% Poloxamer 407 (F127) (HY-D1005) and 2 mM Trypan Red (HY-D0983)).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Cells are plated in clear-bottomed black 96-well microplates.
2.2 Add 100 μL DiBAC4(3) into the cell plate.
2.3 Incubate the dye-loading plate in a cell incubator for 30 to 60 minutes.
2.4 Run the membrane potential assay by monitoring the fluorescence intensity at Ex/Em = 490/505 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

505

Excitation (Ex)

490

Chemical Information

CAS No. 70363-83-6
Appearance Solid
Molecular Weight 516.63
Formula C₂₇H₄₀N₄O₆
Color Pink to red
SMILES

O=C1N(CCCC)C(/C(C(N1CCCC)=O)=C/C=C/C(C(N2CCCC)=O)C(N(CCCC)C2=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (18)

All Cell Imaging/Staining

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Journal Impact Factor

Science

Iron-catalyzed active lipid peroxides drive ultrafast collective cell death in blooming algae. [Abstract]

2026 Jun 25;392(6805):eaed3823. PMID: 42348688
Cell Metab

Tumor-associated macrophages are shaped by intratumoral high potassium via Kir2.1. [Abstract]

2022 Nov 1;34(11):1843-1859.e11. PMID: 36103895
Bioact Mater

Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges. [Abstract]

2025 Nov 25:57:646-659. PMID: 41377894

DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Nov 25:57:646-659. [Abstract]

DiBAC4(3) (300 nM, 30 min) was used for membrane potential measurement in Caco-2 cells.
ACS Nano

Carbon Nanomaterials for Plant Priming through Mechanostimulation: Emphasizing the Role of Shape. [Abstract]

2024 Apr 23;18(16):10829-10839. PMID: 38607639
Food Res Int

Antimicrobial effect of lauroyl arginate ethyl on Listeria monocytogenes and its application in controlling microbial contamination of enoki mushrooms (Flammulina velutipes). [Abstract]

2025 Dec;221(Pt 4):117517. PMID: 41185277

DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Food Res Int. 2025 Dec;221(Pt 4):117517. [Abstract]

DiBAC4(3) at a final concentration of 2.5 μg/mL for 15 min at 25 °C protected from light. was used for membrane potential measurement in L. monocytogenes EGD-e.
ACS Appl Mater Interfaces

Supramolecular Self-Assembled Hydrogel for Antiviral Therapy through Glycyrrhizic Acid-Enhanced Zinc Absorption and Intracellular Accumulation. [Abstract]

2024 Nov 6;16(44):60027-60044. PMID: 39466722
Front Immunol

TENS alleviates CP/CPPS-related inflammation and pain by modulating Kir2.1-dependent macrophage polarization. [Abstract]

2026 Jan 12:16:1683500. PMID: 41624842
J Agric Food Chem

Efficient Production of Natural Yellow Azaphilone Pigments from Monascus purpureus Regulated by Microparticle-Triggered Stress. [Abstract]

2025 Sep 3;73(35):21947-21958. PMID: 40836558
J Oral Microbiol

2023 Dec 10.
Int J Food Microbiol

Antibacterial activity and multi-target mechanism of naringenin against Alicyclobacillus acidoterrestris and its application in apple juice. [Abstract]

2026 Oct 2:459:111897. PMID: 42296754
Food Bioproc Tech

2026 Jun 12;19(7):357.
ACS Omega

In Vitro Inhibition of Growth, Biofilm Formation, and Persisters of Staphylococcus aureus by Pinaverium Bromide. [Abstract]

2023 Mar 6;8(10):9652-9661. PMID: 36936302
ACS Infect Dis

Repurposing GSK2018682 Confers Dual Antibacterial and Antibiofilm Activity against Staphylococcus aureus. [Abstract]

2026 Feb 13;12(2):623-636. PMID: 41525229
ACS Synth Biol

Signal Peptide-Guided Delivery of a Mucin-Like Collagen Analogue for Periplasmic Barrier Reinforcement: A Platform for Enhancing Microbial Survival. [Abstract]

2025 Sep 19;14(9):3767-3783. PMID: 40924575
J Cancer Res Clin Oncol

Hyperbaric oxygen enhanced the chemotherapy of mitochondrial targeting molecule IR-780 in bladder cancer. [Abstract]

2023 Feb;149(2):683-699. PMID: 36436092

DiBAC4(3) purchased from MedChemExpress. Usage Cited in: J Cancer Res Clin Oncol. 2023 Feb;149(2):683-699. [Abstract]

DiBAC4(3) at a final concentration of 100 nM for 20 min was used for membrane potential measurement in T24 cells.
Plasma Process Polym

2026 Mar 31;23(4):e70155.
Biomed Pharmacother

Inhibitory effects and mode of antifungal action of isobavachalcone on Candida albicans growth and virulence factors. [Abstract]

2024 Aug 28:179:117352. PMID: 39208670

DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Aug 28:179:117352. [Abstract]

DiBAC4(3) at a final concentration of 1 μM for 10 min was used for membrane potential measurement in C. albicans SC5314 cells.
Research Square Preprint

2020 Nov.

DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Research Square Preprint. 2020 Nov.

DiBAC4(3) at a final concentration of 100 nM for 20 min at room temperature was used for membrane potential measurement in T24 cells.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (96.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9356 mL

9.6781 mL

19.3562 mL

5 mM

0.3871 mL

1.9356 mL

3.8712 mL

10 mM

0.1936 mL

0.9678 mL

1.9356 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Protocol

Cell Assay^[1]

Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9356 mL	9.6781 mL	19.3562 mL	48.3905 mL
	5 mM	0.3871 mL	1.9356 mL	3.8712 mL	9.6781 mL
	10 mM	0.1936 mL	0.9678 mL	1.9356 mL	4.8391 mL
	15 mM	0.1290 mL	0.6452 mL	1.2904 mL	3.2260 mL
	20 mM	0.0968 mL	0.4839 mL	0.9678 mL	2.4195 mL
	25 mM	0.0774 mL	0.3871 mL	0.7742 mL	1.9356 mL
	30 mM	0.0645 mL	0.3226 mL	0.6452 mL	1.6130 mL
	40 mM	0.0484 mL	0.2420 mL	0.4839 mL	1.2098 mL
	50 mM	0.0387 mL	0.1936 mL	0.3871 mL	0.9678 mL
	60 mM	0.0323 mL	0.1613 mL	0.3226 mL	0.8065 mL
	80 mM	0.0242 mL	0.1210 mL	0.2420 mL	0.6049 mL

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DiBAC4(3)

Publications Citing Use of MedChemExpress (MCE) DiBAC4(3)

Biological Activity

Chemical Information

Publications (18)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

DiBAC4(3) Related Classifications

HY-101892 Related Classifications