Tyrphostin 23
Based on 6 publication(s) in Google Scholar
Tyrphostin 23 (Tyrphostin A23) is an EGFR inhibitor with an IC50 and Kiof 35 and 11 μM, respectively.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 98.19%
- CAS No.: 118409-57-7
- Formule: C10H6N2O2
- Masse moléculaire:186.17
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Tyrphostin 23
More- Nat Commun. 2025 May 27;16(1):4887. [Abstract]
- Nat Commun. 2024 Apr 23;15(1):3437. [Abstract]
- Proc Natl Acad Sci U S A. 2025 Mar 25;122(12):e2420534122. [Abstract]
- PLoS Pathog. 2023 Feb 3;19(2):e1011126. [Abstract]
- J Virol. 2022 Feb 23;96(4):e0191921. [Abstract]
- Pflugers Arch. 2024 Dec;476(12):1913-1928. [Abstract]
Voir tous les produits spécifiques à Isoform EGFR
More
Activité biologique
Description
IC50 & Target
[1]|
EGFR 35 μM (IC50, Cell Assay) |
EGFR 11 μM (Ki) |
In Vitro
10 μM tyrphostin 23 causes significant inhibition of the cell proliferation caused by 1 nM and 1μM ghrelin[2]. Tyrphostin 23 (T23) is a well-known inhibitor of protein tyrosine kinases and has been considered as potential anti-cancer drug. T23 is reported to acutely stimulate the glycolytic flux in primary cultured astrocytes. Tyrphostin 23 stimulates glycolytic flux in cultured astrocytes. Mitochondrial metabolism of brain astrocytes is accelerated by Tyrphostin 23 exposure[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 118409-57-7
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Appearance Solid
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Masse moléculaire 186.17
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Formule C10H6N2O2
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Color Light yellow to green yellow
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SMILES
N#C/C(C#N)=C/C1=CC=C(O)C(O)=C1
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Synonyms
Tyrphostin A23; RG-50810; AG 18
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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Nat Commun
Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly. [Abstract]2025 May 27;16(1):4887. PMID: 40425567 -
Nat Commun
2024 Apr 23;15(1):3437. PMID: 38653755 -
Proc Natl Acad Sci U S A
Wdr5-mediated H3K4 methylation facilitates HSPC development via maintenance of genomic stability in zebrafish. [Abstract]2025 Mar 25;122(12):e2420534122. PMID: 40112113 -
PLoS Pathog
Picornavirus infection enhances aspartate by the SLC38A8 transporter to promote viral replication. [Abstract]2023 Feb 3;19(2):e1011126. PMID: 36735752 -
J Virol
African Swine Fever Virus Regulates Host Energy and Amino Acid Metabolism To Promote Viral Replication. [Abstract]2022 Feb 23;96(4):e0191921. PMID: 34908441 -
Pflugers Arch
Unlocking the potential: unveiling tyrphostins with Michael-reactive cyanoacrylate motif as promising inhibitors of human 5-lipoxygenase. [Abstract]2024 Dec;476(12):1913-1928. PMID: 39347835
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (537.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.43 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (537 KB)
- English - EN (537 KB)
- Français - FR (537 KB)
- Deutsch - DE (537 KB)
- Norwegian - NO (537 KB)
- Español - ES (537 KB)
- Swedish - SV (537 KB)
- Italian - IT (537 KB)
- Korean - KR (537 KB)
- Portuguese - PT (537 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Gazit A, et al. Tyrphostins I: synthesis and biological activity of protein tyrosine kinase inhibitors. J Med Chem. 1989 Oct;32(10):2344-52. [Content Brief]
[2]. Nanzer AM, et al. Ghrelin exerts a proliferative effect on a rat pituitary somatotroph cell line via the mitogen-activated protein kinase pathway. Eur J Endocrinol. 2004 Aug;151(2):233-40. [Content Brief]
[3]. Hohnholt MC, et al. The tricarboxylic acid cycle activity in cultured primary astrocytes is strongly accelerated by the protein tyrosine kinase inhibitor tyrphostin 23. Neurochem Int. 2017 Jan;102:13-21. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.3714 mL | 26.8572 mL | 53.7143 mL | 134.2859 mL |
| 5 mM | 1.0743 mL | 5.3714 mL | 10.7429 mL | 26.8572 mL | |
| 10 mM | 0.5371 mL | 2.6857 mL | 5.3714 mL | 13.4286 mL | |
| 15 mM | 0.3581 mL | 1.7905 mL | 3.5810 mL | 8.9524 mL | |
| 20 mM | 0.2686 mL | 1.3429 mL | 2.6857 mL | 6.7143 mL | |
| 25 mM | 0.2149 mL | 1.0743 mL | 2.1486 mL | 5.3714 mL | |
| 30 mM | 0.1790 mL | 0.8952 mL | 1.7905 mL | 4.4762 mL | |
| 40 mM | 0.1343 mL | 0.6714 mL | 1.3429 mL | 3.3571 mL | |
| 50 mM | 0.1074 mL | 0.5371 mL | 1.0743 mL | 2.6857 mL | |
| 60 mM | 0.0895 mL | 0.4476 mL | 0.8952 mL | 2.2381 mL | |
| 80 mM | 0.0671 mL | 0.3357 mL | 0.6714 mL | 1.6786 mL | |
| 100 mM | 0.0537 mL | 0.2686 mL | 0.5371 mL | 1.3429 mL |
Tyrphostin 23 Related Classifications
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