Alrestatin
Based on 1 publication(s) in Google Scholar
Alrestatin (AY-22284) is an aldose reductase inhibitor. Alrestatin reduces fructose levels in the uterine fluid of mice. Alrestatin interferes with sperm capacitation and impairs fertilization function in mice. Alrestatin decreases basal and tyramine-induced norepinephrine release in rat pancreatic specimens in vitro. Alrestatin enhances glucose- and arginine-stimulated insulin secretion in vivo. Alrestatin can be used in studies related to diabetes and reproductive diseases.
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- Pureza: 99.21%
- No. CAS: 51411-04-2
- Fòrmula: C14H9NO4
- Peso molecular:255.23
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Publications Citing Use of MedChemExpress (MCE) Alrestatin
More
Actividad biológica
Alrestatin (1-5 mg/mL; 15 min) dose-dependently inhibits basal and tyramine-stimulated 3H-norepinephrine release from rat splenic and pancreatic tissue samples in vitro[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Alrestatin (0.15 μg/μL; intrauterine infusion) significantly reduces fructose levels in mouse uterine fluid without altering glucose or sorbitol levels[2].
Alrestatin (1.5 mmol/L; intrauterine injection) significantly reduces sperm capacitation rate and fertilization rate in mice, while co-administration of fructose or sorbitol reverses these effects[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley (male, 200-250 g, fasted, anaesthetized)[1]
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Dosage:0.75 mmol/kg
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Administration:i.v.; single bolus
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Result:Produced significantly greater mean incremental plasma insulin levels compared to vehicle.
Reduced mean incremental plasma glucose levels significantly compared to vehicle.
Did not significantly alter basal fasting plasma glucose or insulin levels relative to vehicle when administered without glucose.
Chemical Information
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No. CAS 51411-04-2
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Appearance Solid
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Peso molecular 255.23
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Fòrmula C14H9NO4
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Color White to off-white
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SMILES
O=C(O)CN(C(C1=CC=CC2=CC=CC3=C12)=O)C3=O
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Synonyms
AY-22284
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Theriogenology
Expression of aldose reductase in mouse endometrial epithelial cells and its role in sperm capacitation. [Abstract]2023 Oct 1:209:243-250. PMID: 37480702
Solvente y solubilidad
DMSO : 50 mg/mL (195.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.80 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (302 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Kobric M, et al. Effect of alrestatin sodium on glucose-stimulated insulin secretion in the fasted anaesthetized rat. Horm Metab Res. 1978;10(6):495-500. [Content Brief]
[2]. Lei Y, et al. Expression of aldose reductase in mouse endometrial epithelial cells and its role in sperm capacitation. Theriogenology. 2023;209:243-250. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9180 mL | 19.5902 mL | 39.1803 mL | 97.9509 mL |
| 5 mM | 0.7836 mL | 3.9180 mL | 7.8361 mL | 19.5902 mL | |
| 10 mM | 0.3918 mL | 1.9590 mL | 3.9180 mL | 9.7951 mL | |
| 15 mM | 0.2612 mL | 1.3060 mL | 2.6120 mL | 6.5301 mL | |
| 20 mM | 0.1959 mL | 0.9795 mL | 1.9590 mL | 4.8975 mL | |
| 25 mM | 0.1567 mL | 0.7836 mL | 1.5672 mL | 3.9180 mL | |
| 30 mM | 0.1306 mL | 0.6530 mL | 1.3060 mL | 3.2650 mL | |
| 40 mM | 0.0980 mL | 0.4898 mL | 0.9795 mL | 2.4488 mL | |
| 50 mM | 0.0784 mL | 0.3918 mL | 0.7836 mL | 1.9590 mL | |
| 60 mM | 0.0653 mL | 0.3265 mL | 0.6530 mL | 1.6325 mL | |
| 80 mM | 0.0490 mL | 0.2449 mL | 0.4898 mL | 1.2244 mL | |
| 100 mM | 0.0392 mL | 0.1959 mL | 0.3918 mL | 0.9795 mL |
Alrestatin Related Classifications
HY-B1202 Related Classifications
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