CHAPS
Based on 4 publication(s) in Google Scholar
CHAPS is a cholic acid-derived, sulfobetaine-type zwitterionic detergent and micelle-forming agent. CHAPS exhibits properties of weak cationic or nonionic surfactants in different solution systems, undergoes micellization, and forms small, loose hydrophilic aggregates that are temperature-dependent. CHAPS stabilizes mononucleosomes under different ionic strengths, reduces nucleosome sequence specificity, promotes sliding of histone cores along DNA, solubilizes Tamm-Horsfall protein to reduce its interference with urinary exosome isolation, and maintains vesicle structure and the activity of related proteins at the same time. CHAPS is used to recover native folded fusion proteins, enhance the binding capacity of GST fusion proteins, and restore GST enzyme activity. However, CHAPS cannot refold proteins denatured by urea, guanidine hydrochloride or heat, nor can it construct the structure of intrinsically disordered proteins. CHAPS is commonly used in research on the separation and purification of membrane proteins.
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- Pureza: 99.91%
- No. CAS: 75621-03-3
- Fòrmula: C32H58N2O7S
- Peso molecular:614.88
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CHAPS
More
Actividad biológica
CHAPS forms low aggregation number (5.4-6.7) micelles with cmc values dependent on solvent and temperature (U-shaped cmc vs temperature curve with minimum at ~295 K) and ΔHm0 transitioning from endothermic to exothermic with increasing temperature in aqueous solutions, NaCl solutions, and buffer solutions[1].
CHAPS (16-4.8 mM; 1-120 h) stabilizes reconstituted mononucleosomes (assembled with human histones H2A, H2B, H3, H4 on 601 sequence DNA) at 0.4 nM concentration at 0°C without altering their structure, with 16 μM preventing dissociation for 1 hour, 1.6 mM maintaining over 50% intact nucleosomes for 120 hours, and 4.8 mM maintaining over 70% intact nucleosomes for 120 hours[2].
CHAPS (1% (w/v); overnight at 4°C) treatment of pooled urine-derived exosomal pellets (P18, P200) preserves vesicle morphology, exosomal marker distribution, and nephrilysin activity while removing interfering proteins like THP and albumin[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 75621-03-3
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Appearance Solid
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Peso molecular 614.88
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Fòrmula C32H58N2O7S
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Color White to off-white
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SMILES
O[C@@H]1CC[C@]2(C)[C@@]3([H])C[C@H](O)[C@]4(C)[C@@H]([C@H](C)CCC(NCCC[N+](C)(C)CCCS([O-])(=O)=O)=O)CC[C@@]4([H])[C@]3([H])[C@H](O)C[C@@]([H])2C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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J Colloid Interface Sci
Efficient biofunctionalization of MoS2 nanosheets with peptides as intracellular fluorescent biosensor for sensitive detection of caspase-3 activity. [Abstract]2019 May 1:543:96-105. PMID: 30782521 -
Brain Behav Immun
2026 Feb 24:135:106516. PMID: 41747779 -
Biochem Pharmacol
2025 Apr 12:116941. PMID: 40228633 -
Biochem Pharmacol
2024 Jun 26:226:116392. PMID: 38942091
Solvente y solubilidad
DMSO : 116.67 mg/mL (189.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 66.67 mg/mL (108.43 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 10 mg/mL (16.26 mM); Clear solution; Need ultrasonic
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Kroflič A, et al. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012;28(28):10363-10371. [Content Brief]
[2]. Menshikova I, et al. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2(2):129-137. [Content Brief]
[3]. Musante L, et al. Biochemical and physical characterisation of urinary nanovesicles following CHAPS treatment. PLoS One. 2012;7(7):e37279. [Content Brief]
[4]. Tao H, et al. Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS. Biotechniques. 2010;48(1):61-64. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.6263 mL | 8.1317 mL | 16.2633 mL | 40.6583 mL |
| 5 mM | 0.3253 mL | 1.6263 mL | 3.2527 mL | 8.1317 mL | |
| 10 mM | 0.1626 mL | 0.8132 mL | 1.6263 mL | 4.0658 mL | |
| 15 mM | 0.1084 mL | 0.5421 mL | 1.0842 mL | 2.7106 mL | |
| 20 mM | 0.0813 mL | 0.4066 mL | 0.8132 mL | 2.0329 mL | |
| 25 mM | 0.0651 mL | 0.3253 mL | 0.6505 mL | 1.6263 mL | |
| 30 mM | 0.0542 mL | 0.2711 mL | 0.5421 mL | 1.3553 mL | |
| 40 mM | 0.0407 mL | 0.2033 mL | 0.4066 mL | 1.0165 mL | |
| 50 mM | 0.0325 mL | 0.1626 mL | 0.3253 mL | 0.8132 mL | |
| 60 mM | 0.0271 mL | 0.1355 mL | 0.2711 mL | 0.6776 mL | |
| 80 mM | 0.0203 mL | 0.1016 mL | 0.2033 mL | 0.5082 mL | |
| 100 mM | 0.0163 mL | 0.0813 mL | 0.1626 mL | 0.4066 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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