DiBAC4(3)
Based on 18 publication(s) in Google Scholar
DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm). DiBAC4(3) can be used to measure cell membrane potential.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.91%
- No. CAS: 70363-83-6
- Fòrmula: C27H40N4O6
- Peso molecular:516.63
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DiBAC4(3)
More- Science. 2026 Jun 25;392(6805):eaed3823. [Abstract]
- Cell Metab. 2022 Nov 1;34(11):1843-1859.e11. [Abstract]
- Bioact Mater. 2025 Nov 25:57:646-659. [Abstract]
- ACS Nano. 2024 Apr 23;18(16):10829-10839. [Abstract]
- Food Res Int. 2025 Dec;221(Pt 4):117517. [Abstract]
- ACS Appl Mater Interfaces. 2024 Nov 6;16(44):60027-60044. [Abstract]
- Front Immunol. 2026 Jan 12:16:1683500. [Abstract]
- J Agric Food Chem. 2025 Sep 3;73(35):21947-21958. [Abstract]
- J Oral Microbiol. 2023 Dec 10.
- Int J Food Microbiol. 2026 Oct 2:459:111897. [Abstract]
- Food Bioproc Tech. 2026 Jun 12;19(7):357.
- ACS Omega. 2023 Mar 6;8(10):9652-9661. [Abstract]
- ACS Infect Dis. 2026 Feb 13;12(2):623-636. [Abstract]
- ACS Synth Biol. 2025 Sep 19;14(9):3767-3783. [Abstract]
- J Cancer Res Clin Oncol. 2023 Feb;149(2):683-699. [Abstract]
- Plasma Process Polym. 2026 Mar 31;23(4):e70155.
- Biomed Pharmacother. 2024 Aug 28:179:117352. [Abstract]
- Research Square Preprint. 2020 Nov.
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
Actividad biológica
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 10-40 mM.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 10-40 μM with Hanks and 20 mM Hepes buffer (HHBS) or other buffer, such as pH 7 with 0.04% to 0.08% Poloxamer 407 (F127) (HY-D1005) and 2 mM Trypan Red (HY-D0983)).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Cells are plated in clear-bottomed black 96-well microplates.
2.2 Add 100 μL DiBAC4(3) into the cell plate.
2.3 Incubate the dye-loading plate in a cell incubator for 30 to 60 minutes.
2.4 Run the membrane potential assay by monitoring the fluorescence intensity at Ex/Em = 490/505 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
505
490
Chemical Information
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No. CAS 70363-83-6
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Appearance Solid
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Peso molecular 516.63
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Fòrmula C27H40N4O6
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Color Pink to red
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SMILES
O=C1N(CCCC)C(/C(C(N1CCCC)=O)=C/C=C/C(C(N2CCCC)=O)C(N(CCCC)C2=O)=O)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (18)
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Journal Impact Factor
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Most Recent
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Science
Iron-catalyzed active lipid peroxides drive ultrafast collective cell death in blooming algae. [Abstract]2026 Jun 25;392(6805):eaed3823. PMID: 42348688 -
Cell Metab
2022 Nov 1;34(11):1843-1859.e11. PMID: 36103895 -
Bioact Mater
Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges. [Abstract]2025 Nov 25:57:646-659. PMID: 41377894
DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2025 Nov 25:57:646-659. [Abstract]
DiBAC4(3) (300 nM, 30 min) was used for membrane potential measurement in Caco-2 cells.
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ACS Nano
Carbon Nanomaterials for Plant Priming through Mechanostimulation: Emphasizing the Role of Shape. [Abstract]2024 Apr 23;18(16):10829-10839. PMID: 38607639 -
Food Res Int
Antimicrobial effect of lauroyl arginate ethyl on Listeria monocytogenes and its application in controlling microbial contamination of enoki mushrooms (Flammulina velutipes). [Abstract]2025 Dec;221(Pt 4):117517. PMID: 41185277
DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Food Res Int. 2025 Dec;221(Pt 4):117517. [Abstract]
DiBAC4(3) at a final concentration of 2.5 μg/mL for 15 min at 25 °C protected from light. was used for membrane potential measurement in L. monocytogenes EGD-e.
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ACS Appl Mater Interfaces
Supramolecular Self-Assembled Hydrogel for Antiviral Therapy through Glycyrrhizic Acid-Enhanced Zinc Absorption and Intracellular Accumulation. [Abstract]2024 Nov 6;16(44):60027-60044. PMID: 39466722 -
Front Immunol
TENS alleviates CP/CPPS-related inflammation and pain by modulating Kir2.1-dependent macrophage polarization. [Abstract]2026 Jan 12:16:1683500. PMID: 41624842 -
J Agric Food Chem
Efficient Production of Natural Yellow Azaphilone Pigments from Monascus purpureus Regulated by Microparticle-Triggered Stress. [Abstract]2025 Sep 3;73(35):21947-21958. PMID: 40836558 -
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Int J Food Microbiol
Antibacterial activity and multi-target mechanism of naringenin against Alicyclobacillus acidoterrestris and its application in apple juice. [Abstract]2026 Oct 2:459:111897. PMID: 42296754 -
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ACS Omega
In Vitro Inhibition of Growth, Biofilm Formation, and Persisters of Staphylococcus aureus by Pinaverium Bromide. [Abstract]2023 Mar 6;8(10):9652-9661. PMID: 36936302 -
ACS Infect Dis
Repurposing GSK2018682 Confers Dual Antibacterial and Antibiofilm Activity against Staphylococcus aureus. [Abstract]2026 Feb 13;12(2):623-636. PMID: 41525229 -
ACS Synth Biol
Signal Peptide-Guided Delivery of a Mucin-Like Collagen Analogue for Periplasmic Barrier Reinforcement: A Platform for Enhancing Microbial Survival. [Abstract]2025 Sep 19;14(9):3767-3783. PMID: 40924575 -
J Cancer Res Clin Oncol
Hyperbaric oxygen enhanced the chemotherapy of mitochondrial targeting molecule IR-780 in bladder cancer. [Abstract]2023 Feb;149(2):683-699. PMID: 36436092
DiBAC4(3) purchased from MedChemExpress. Usage Cited in: J Cancer Res Clin Oncol. 2023 Feb;149(2):683-699. [Abstract]
DiBAC4(3) at a final concentration of 100 nM for 20 min was used for membrane potential measurement in T24 cells.
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Biomed Pharmacother
Inhibitory effects and mode of antifungal action of isobavachalcone on Candida albicans growth and virulence factors. [Abstract]2024 Aug 28:179:117352. PMID: 39208670
DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 Aug 28:179:117352. [Abstract]
DiBAC4(3) at a final concentration of 1 μM for 10 min was used for membrane potential measurement in C. albicans SC5314 cells.
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DiBAC4(3) purchased from MedChemExpress. Usage Cited in: Research Square Preprint. 2020 Nov.
DiBAC4(3) at a final concentration of 100 nM for 20 min at room temperature was used for membrane potential measurement in T24 cells.
Solvente y solubilidad
DMSO : 50 mg/mL (96.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocolo
Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9356 mL | 9.6781 mL | 19.3562 mL | 48.3905 mL |
| 5 mM | 0.3871 mL | 1.9356 mL | 3.8712 mL | 9.6781 mL | |
| 10 mM | 0.1936 mL | 0.9678 mL | 1.9356 mL | 4.8391 mL | |
| 15 mM | 0.1290 mL | 0.6452 mL | 1.2904 mL | 3.2260 mL | |
| 20 mM | 0.0968 mL | 0.4839 mL | 0.9678 mL | 2.4195 mL | |
| 25 mM | 0.0774 mL | 0.3871 mL | 0.7742 mL | 1.9356 mL | |
| 30 mM | 0.0645 mL | 0.3226 mL | 0.6452 mL | 1.6130 mL | |
| 40 mM | 0.0484 mL | 0.2420 mL | 0.4839 mL | 1.2098 mL | |
| 50 mM | 0.0387 mL | 0.1936 mL | 0.3871 mL | 0.9678 mL | |
| 60 mM | 0.0323 mL | 0.1613 mL | 0.3226 mL | 0.8065 mL | |
| 80 mM | 0.0242 mL | 0.1210 mL | 0.2420 mL | 0.6049 mL |
DiBAC4(3) Related Classifications
HY-101892 Related Classifications
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